Publication Type |
pre-print |
School or College |
College of Science |
Department |
Biology |
Creator |
Jorgensen, Erik |
Other Author |
Watanabe, Shigeki; Richards, Jackson; Hollopeter, Gunther; Hobson, Robert J.; Davis, Wayne M. |
Title |
Nano-fEM: Protein localization using photo-activated localization microscopy and electron microscopy |
Date |
2012-01-01 |
Description |
Mapping the distribution of proteins is essential for understanding the function of proteins in a cell. Fluorescence microscopy is extensively used for protein localization, but subcellular context is often absent in fluorescence images. Immuno-electron microscopy, on the other hand, can localize proteins, but the technique is limited by a lack of compatible antibodies, poor preservation of morphology and because most antigens are not exposed to the specimen surface. Correlative approaches can acquire the fluorescence image from a whole cell first, either from immunofluorescence or genetically tagged proteins. The sample is then fixed and embedded for electron microscopy, and the images are correlated 1-3. However, the low-resolution fluorescence image and the lack of fiducial markers preclude the precise localization of proteins. |
Type |
Text |
Publisher |
Journal of Visualized Experiments (JoVE) |
Volume |
70 |
First Page |
1 |
Last Page |
8 |
Language |
eng |
Bibliographic Citation |
Watanabe, S., Richards, J., Hollopeter, G., Hobson, R. J., Davis, W. M., & Jorgensen, E. M. (2012). Nano-fEM: Protein localization using photo-activated localization microscopy and electron microscopy. Journal of Visualized Experiments, 70, e3995, 1-8. |
Format Medium |
application/pdf |
Format Extent |
1,158,023 bytes |
Identifier |
uspace,18127 |
ARK |
ark:/87278/s60580qp |
Setname |
ir_uspace |
ID |
708336 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s60580qp |