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CreatorTitleDescriptionSubjectDate
76 Owen, William E.; Roberts, William L.Performance characteristics of an HPLC method for Urinary AlbuminMicroalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detecti...MA; Microalbuminuria; HPLC2005-07-06
77 Owen, William E.; Roberts, William L.Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods.Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur...Glycohemoglobin; GHB; Glycated Hb; Glycemic control2008
78 Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T.Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis [abstract]This abstract describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the meth...Genotyping; Amplicon Melting2005-06-22
79 Owen, William E.; Rockwood, Alan L.; Roberts, William L.; Meikle, A. WayneDevelopment and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroidsCongenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17O...Congenital adrenal hyperplasia; Adrenal steroids; Tandem mass spectrometry assay2006
80 Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T.Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysisThis poster describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the method...Genotyping; Amplicon Melting2005-06
81 Hymas, Weston C.; Hillyard, David R.Novel one step real time RT-PCR assay for the detection of Enterovirus, AThis poster describes the development of a novel real time assay that utilizes primers and an Eclipse probe in an overlapping format upstream of the Rotbart amplicon.RT-PCR Assay2005-11-08
82 Margraf, Rebecca L.; Mao, Rong; Wittwer, Carl T.Masking techniques: masking selected sequence variation by incorporating mismatches into melting analysis probesHybridization probe melting analysis can be complicated by the presence of sequence variation (non-pathogenic polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Materials and Meth...probe hybridization; high-resolution melting analysis; masking; RET2005-10-21
83 Margraf, Rebecca L.; Mao, Rong; Highsmith, W. Edward; Holtegaard, Leonard; Wittwer, Carl T.Masking selected sequence variation by incorporating mismatches into melting analysis probesHybridization probe melting analysis can be complicated by the presence of sequence variations (benign polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Selected sequence variati...probe hybridization; high-resolution melting analysis; masking; RET2005-06-28
84 Stevenson, Jeffery B.; Hillyard, David R.Quantitative real time PCR assay for detecting EBV virus in multiple sample types.We are developing a real time quantitative PCR assay to detect EBV in serum, plasma, whole blood, tissue and spinal fluid. Real time PCR, with its intrinsic quantitative capacity, is an excellent method for measuring EBV viral load. Epstein Barr virus is a member of the Herpesvirus family, with a tr...Eclipse Probes; EBV Primer2005-08-11
85 Digre, Kathleen B.; Gouw, Launce G.; Harris, Catherine P.; Haines, John H.; Ptacek, L.J.Autosomal dominant cerebellar ataxia with retinal degeneration: clinical, neuropathologic, and genetic analysis of a large kindred.The autosomal dominant cerebellar ataxias (ADCA) comprise a heterogeneous group of neurologic disorders characterized by degeneration of the cerebellum, spinal cord, and brainstem. Genetic analysis has revealed two loci, SCA1 on chromosome 6p, and SCA2 on chromosome 12q, responsible for some ADCA. W...Retinal Degeneration; Cerebellar Ataxia; Genetic Analysis1994-08
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