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51 Smith, A. Gordon; Singleton, J. RobinsonLifestyle intervention for pre-diabetic neuropathy.OBJECTIVE: The purpose of this study was to evaluate intraepidermal nerve fiber density (IENFD) as a sensitive measure of neuropathy change in patients with neuropathy associated with impaired glucose tolerance (IGT) receiving lifestyle intervention based on that used in the Diabetes Prevention Prog...Aged; Biopsy; Blood Pressure; Body Mass Index; Cholesterol; Diabetic Diet2006-06-29
52 Owen, William E.; Martins, Thomas B.; Litwin, Christine M.; Roberts, William L.Performance characteristics of six IMMULITE 2000 TORCH assaysTORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (like syphilis), rubella (RV), cytomegalovirus (CMV), and herpesviruses (HSV) that are associated with congenital abnormalities during maternal infection. Prenatal testing for antibodies against these agents is one tool for preve...TORCH; Toxoplasma gondii; syphilis virus; rubella; cytomegalovirus; herpesvirus; IMMULITE 2000; congenital abnormalities; laboratory testing2006-01-04
53 Owen, William E.; Roberts, William L.Performance characteristics of eight estradiol immunoassaysMeasurement of estradiol is useful in assisted reproduction, evaluation of infertility, menopause, and male feminization. The analytic performance of 8 estradiol immunoassays was evaluated. The imprecision and accuracy of the Access, ADVIA Centaur, ARCHITECT i2000, AutoDELFIA, Elecsys 2010, IMMULI...Estradiol immunoassays2004
54 Wittwer, Carl T.Genotyping accuracy of high-resolution DNA melting instrumentsHigh-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assesse...2014-01-01
55 Dwight, Zachary LawrenceuAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictionsAbstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce...2012-01-01
56 Owen, William E.; Roberts, William L.Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluidMeasurement of IgA and IgM in cerebrospinal fluid (CSF) can be useful in the diagnosis of multiple sclerosis and other central nervous system disorders. The Dade Behring (Deerfield, IL) N Latex IgA and N Latex IgM tests on the BN II System and Beckman Coulter (Brea, CA) low-concentration IgA an...IgA; IgM; Immunonephelometric assays; Imprecision; Method comparison; Reference interval2003
57 Jaskowski, Troy D.Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassayWe used a multiplexed fluorescent microsphere immunoassay to develop a sandwich capture assay to assess simultaneously the production of thymus helper (TH) 1- and TH2-type cytokines in tissue culture supernatant obtained from stimulated peripheral blood mononuclear cells. The assay then was use...Multiplexed fluorescent microsphere immunoassay2002
58 Normann, Richard A.; Chandler, John P.Effects of calcium ions on L-type horizontal cells in the isolated turtle retinaA technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p...Retina; Calcium; Horizontal Cell; Turtle1990
59 Jaskowski, Troy D.Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination controlToxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction...PCR probe capture assay; Uracil N-glycosylase; UNG2000
60 Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M.Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysisDifferentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar...MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR2006-01-09
61 Jaskowski, Troy D.Comparison of four enzyme immunoassays with a western blot assay for the determination of type-specific antibodies to herpes simplex virusMost current enzyme immunoassays (EIAs) differentiate inadequately between types 1 and 2 herpes simplex virus (HSV) antibodies since significant crossreactivity exists. We compared 4 IgG type-specific EIAs using a Western blot assay for resolution of discrepant results. The Diamedix had sensitivit...Enzyme immunoassays; Western blot assay; Type-specific antibodies2001
62 Coffin, Cheryl M.; Capecchi, Mario R.Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function.Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has...Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors2004-11-01
63 Aldous, Jay A.; Engar, Richard C.Do dentists prescribe narcotics excessively?Dealing with pain is an inevitable sequela to dental treatment. Although several drug regimens primarily involving narcotics have been used in the past, availability of nonsteroidal anti-inflammatory drugs (NSAIDs) has increased recently. A study was conducted to analyze dental prescribing patterns ...Analgesics, Opioid; Anti-Inflammatory Agents; Dentist's Practice Patterns; Pain, Postoperative1996-07-01
64 Hymas, Weston C.; Hillyard, David R.Real time RT-PCR assay for norovirus detection with eclipse probes and a non-competitive internal control.Noroviruses are the major causative agents of nonbacterial gastroenteritis worldwide and are estimated to cause approximately 23 million cases in the United States annually. Real time RT-PCR is rapidly becoming the principle diagnostic means for norovirus detection due to its enhanced sensitivity an...Gastroenteritis; Eclipse Hybridization Probes; Norovirus2005-05-03
65 Hymas, Weston C.; Hillyard, David R.Novel one step real time RT-PCR Assay for the detection of Enterovirusnteroviruses (EV) are the leading cause of aseptic meningitis in pediatric and adult populations and can be associated with severe disease such as myocarditis, encephalitis, and paralytic poliomyelitis. RT-PCR has rapidly become the diagnostic methodology of choice due to its sensitivity and rapid t...Viruses; Isolation; Purification2005-07-11
66 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) Assay and Comparison to the COBAS Amplicor HBV Monitor Assay.erformance characteristics of the COBAS HBV TaqMan ASR (TaqMan) assay were evaluated and compared to the COBAS Amplicor HBV Monitor test (Amplicor) assay for the quantification of HBV DNA in clinical samples.Quantitative Tests; Linearity Studies2004-05-03
67 Aldous, Wade K.; Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Edward W.;Hillyard, David R.Single tube real-time RT-PCR Assay for the detection of enterovirusWe developed a rapid and sensitive method for the routine detection of enterovirus in CSF and plasma using a one tube, one step, real-time PCR assay. A primer set and an Eclipse probe were designed to target the highly conserved 5' untranslated region of the enterovirus genome. This assay was compar...MGB Eclipse; Viral RNA; Picornaviridae2004-09-11
68 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay.To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international...Quantitative Tests; Linearity Studies2004-05-10
69 Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R.; Hillyard, David R.Performance Characteristics of the COBAS HCV TaqMan ASR Using Armored RNA CalibratorsAs improved therapies have become available for Hepatititis C Virus (HCV) infection, the use of assays to quantitate HCV RNA has increased dramatically (3, 14, 16). The initial use of these assays was to predict the likelihood of therapy based on baseline HCV RNA levels. These reports indicated that...Roche Amplicor Monitor; NGI Superquant; patients2003-04-22
70 Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R; Hillyard, David RPerformance characteristics of the COBAS HCV TaqMan ASR and Comparison to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0.Evaluate the COBAS HCV TaqMan ASR assay using both the Qiagen BioRobot 9604 and a 1mL extraction protocol for HCV RNA isolation. And Compare the COBAS TaqMan HCV ASR assay to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0 assays.Quantitative Tests; Linearity Studies2002-11-21
71 Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Bill; Hillyard, David R.Quantitative real time PCR assay for the detection of human herpes 6.Human herpes virus type 6 is a beta virus that occurs as two variants (A & B) and infects nearly 100% of the population before two years of age. Primary infections in children can produce fever and a unique lacy rash that gives the disease its name: roseola. The rare primary infection in adults can ...Probe Technology; Roseola Infantum2005-02-05
72 Parker, Sam; Stevenson, Jeffery B.; Hillyard, David R.Eclipse probe assay for the detection of mycoplasma pneumoniae.Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. ...Eclipse Hybridization Probe; Protocol; Techniques2005-09-11
73 Wittwer, Carl T.Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instrumentsBACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS: A continuous assay that monitors DNA polymerase extension us...2014-01-01
74 Rockwood, Alan L.Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometryBackground: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show...2014-01-01
75 Roberts, William L.Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principlesStandardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances.2012
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