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Creator | Title | Description | Subject | Date |
51 |
 | Cloud, Joann L.; Meyer, Jay J.; Pounder, June I.; Woods, Gail L. | Mycobacterium arupense sp. nov., a novel moderately growing nonchromogenic bacterium isolated from clinical specimens | This author manuscript discusses how several isolates of Mycobacterium species related to the M. terrae complex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximate first 500 base pairs) is often used to identify Mycobacteri... | Mycobacterium arupense; Mycobacterium nonchromogenicum; Mycobacterium terrae; Mycobacterium triviale; MCRO 6 | 2005-11-22 |
52 |
 | Litwin, Christine M. | Anti-tuberculosis IgG antibodies as a marker of active mycobacterium tuberculosis disease | Anti-Mycobacterium tuberculosis IgG antibodies may aid in the diagnosis of active M. tuberculosis disease. We studied whether anti-M. tuberculosis IgG antibodies are elevated in active M. tuberculosis disease and assessed factors contributing to false positive and negative results. A retrospective s... | | 2012-01-01 |
53 |
 | Dwight, Zachary Lawrence | uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application | uMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono... | | 2011 |
54 |
 | Jaskowski, Troy D. | Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responses | The Author's have developed a simple, nested polymerase chain reaction (PCR) assay for amplification of an outer surface protein A (OspA) gene fragment of Borrelia burgdorferi using rapid temperature cycling and ethidium bromide detection on agarose gels, and applied it to the diagnosis of Lyme dis... | PCR; Rapid cycle amplification | 1996 |
55 |
 | Dwight, Zachary Lawrence | Laboratory driven, lean-to-adaptive prototyping in Parallel for Web software project identification and application development in health science research | Clinical research laboratories, bioinformatics core facilities, and health science organizations often rely on heavy planning based software development models to propose, build, and distribute software as a consumable product. Projects in non-agile software life cycles tend to have rigid ?plan-desi... | | 2012 |
56 |
 | Owen, William E.; Roberts, William L.; Ring, Terry Arthur | Development and validation of an automated thawing and mixing workcell | Working toward a goal of total laboratory automation, we are automating manual activities in our highest volume laboratory section. Because half of all specimens arriving in this laboratory section are frozen, we began by developing an automated workcell for thawing frozen specimens and mixing the t... | Automated workcell; Laboratory automation | 2007 |
57 |
 | Roberts, William L. | Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principles | Standardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances. | | 2012 |
58 |
 | Owen, William E.; Roberts, William L. | Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods. | Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur... | Glycohemoglobin; GHB; Glycated Hb; Glycemic control | 2008 |
59 |
 | Hymas, Weston C.; Hillyard, David R. | Real time RT-PCR assay for norovirus detection with eclipse probes and a non-competitive internal control. | Noroviruses are the major causative agents of nonbacterial gastroenteritis worldwide and are estimated to cause approximately 23 million cases in the United States annually. Real time RT-PCR is rapidly becoming the principle diagnostic means for norovirus detection due to its enhanced sensitivity an... | Gastroenteritis; Eclipse Hybridization Probes; Norovirus | 2005-05-03 |
60 |
 | Hymas, Weston C.; Hillyard, David R. | Novel one step real time RT-PCR Assay for the detection of Enterovirus | nteroviruses (EV) are the leading cause of aseptic meningitis in pediatric and adult populations and can be associated with severe disease such as myocarditis, encephalitis, and paralytic poliomyelitis. RT-PCR has rapidly become the diagnostic methodology of choice due to its sensitivity and rapid t... | Viruses; Isolation; Purification | 2005-07-11 |
61 |
 | Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R. | Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) Assay and Comparison to the COBAS Amplicor HBV Monitor Assay. | erformance characteristics of the COBAS HBV TaqMan ASR (TaqMan) assay were evaluated and compared to the COBAS Amplicor HBV Monitor test (Amplicor) assay for the quantification of HBV DNA in clinical samples. | Quantitative Tests; Linearity Studies | 2004-05-03 |
62 |
 | Aldous, Wade K.; Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Edward W.;Hillyard, David R. | Single tube real-time RT-PCR Assay for the detection of enterovirus | We developed a rapid and sensitive method for the routine detection of enterovirus in CSF and plasma using a one tube, one step, real-time PCR assay. A primer set and an Eclipse probe were designed to target the highly conserved 5' untranslated region of the enterovirus genome. This assay was compar... | MGB Eclipse; Viral RNA; Picornaviridae | 2004-09-11 |
63 |
 | Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R. | Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay. | To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international... | Quantitative Tests; Linearity Studies | 2004-05-10 |
64 |
 | Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R.; Hillyard, David R. | Performance Characteristics of the COBAS HCV TaqMan ASR Using Armored RNA Calibrators | As improved therapies have become available for Hepatititis C Virus (HCV) infection, the use of assays to quantitate HCV RNA has increased dramatically (3, 14, 16). The initial use of these assays was to predict the likelihood of therapy based on baseline HCV RNA levels. These reports indicated that... | Roche Amplicor Monitor; NGI Superquant; patients | 2003-04-22 |
65 |
 | Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R; Hillyard, David R | Performance characteristics of the COBAS HCV TaqMan ASR and Comparison to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0. | Evaluate the COBAS HCV TaqMan ASR assay using both the Qiagen BioRobot 9604 and a 1mL extraction protocol for HCV RNA isolation. And Compare the COBAS TaqMan HCV ASR assay to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0 assays. | Quantitative Tests; Linearity Studies | 2002-11-21 |
66 |
 | Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Bill; Hillyard, David R. | Quantitative real time PCR assay for the detection of human herpes 6. | Human herpes virus type 6 is a beta virus that occurs as two variants (A & B) and infects nearly 100% of the population before two years of age. Primary infections in children can produce fever and a unique lacy rash that gives the disease its name: roseola. The rare primary infection in adults can ... | Probe Technology; Roseola Infantum | 2005-02-05 |
67 |
 | Parker, Sam; Stevenson, Jeffery B.; Hillyard, David R. | Eclipse probe assay for the detection of mycoplasma pneumoniae. | Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. ... | Eclipse Hybridization Probe; Protocol; Techniques | 2005-09-11 |
68 |
 | Cloud, Joann L.; Hoggan, Karen; Cohen, Samuel; Brown-Elliott, Barbara A.; Mann, Linda; Wilson, Rebecca; Aldous, Wade K.; Wallace, Jr, Richard J.; Woods, Gail L. | Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probes | This poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is ... | MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus | 2005-09-05 |
69 |
 | Slev, Patricia R.; Rawlins, Mindy, L.; Roberts, William L. | Performance characteristics of seven automated CA 15-3 methods | Poster describing studies to evaluate seven automated methods for the following parameters: limit of detection, linearity, imprecision, reference intervals and method comparison with the ADVIA Centaur as the comparison method to detect CA 15-3. | CA 15-3 Method | 2005-07-13 |
70 |
 | Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T. | Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis | This poster describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the method... | Genotyping; Amplicon Melting | 2005-06 |
71 |
 | Erickson, J. Alan; Ashwood, Edward R. | Evaluation of an enzyme-linked binding protein assay for hyaluronic acid and concentrations in hepatitis C infected patients | Serological hyaluronic acid (HA) has been proposed as a noninvasive alternative to liver biopsy for assessing the extent of liver fibrosis. Liver biopsy correctly identifies hepatic disease in about 65 to 75% of cases, being strongly dependent on the length of the biopsy obtained. Furthermore, hepat... | Hyaluronic Acid Concentration; Corgenix HA assay | 2005-07-21 |
72 |
 | Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R.; Hillyard, David R. | Comparison of the National Genetics Institute (NGI) HCV Superquant and Roche COBAS Amplicor HCV Monitor, Version 2.0 Assays | Chronic Hepatitis C infection is currently recognized as an important health care problem. It is estimated that nearly 4 million Americans are infected, 15 to 20 percent of whom will eventually develop Cirrhosis. Current treatments for Hepatitis C virus (HCV) are limited to alfa-interferon alone or ... | Cirrhosis; Patients | 2000-04-22 |
73 |
 | Stevenson, Jeffery B.; Hillyard, David R. | Quantitative real time PCR assay for detecting EBV virus in multiple sample types. | We are developing a real time quantitative PCR assay to detect EBV in serum, plasma, whole blood, tissue and spinal fluid. Real time PCR, with its intrinsic quantitative capacity, is an excellent method for measuring EBV viral load. Epstein Barr virus is a member of the Herpesvirus family, with a tr... | Eclipse Probes; EBV Primer | 2005-08-11 |
74 |
 | Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V. | Cross platform comparison of melting curve analysis | Melting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt... | PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping | 2005-11-04 |
75 |
 | Hymas, Weston C.; Hillyard, David R. | Novel one step real time RT-PCR assay for the detection of Enterovirus, A | This poster describes the development of a novel real time assay that utilizes primers and an Eclipse probe in an overlapping format upstream of the Rotbart amplicon. | RT-PCR Assay | 2005-11-08 |