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Creator | Title | Description | Subject | Date |
| 51 |
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Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R. | Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) and COBAS HCV TaqMan ASR Assays using the Vidiera NSP sample preparation instrument. | The Vidiera Nsp nucleic sample preparation instrument (Vidiera,Beckman-Coulter Inc., Fullerton CA) was evaluated using the COBAS HBV TaqMan Analyte Specific Reagent (ASR. Roche Molecular Diagnostics [RMD], Indianapolis, IN) and COBAS HCV TaqMan ASR (RMD) assays using clinical samples and HCV Armored... | Quantitative Tests; Linearity Studies | 2006-05-05 |
| 52 |
 |
Smith, A. Gordon; Singleton, J. Robinson | Lifestyle intervention for pre-diabetic neuropathy. | OBJECTIVE: The purpose of this study was to evaluate intraepidermal nerve fiber density (IENFD) as a sensitive measure of neuropathy change in patients with neuropathy associated with impaired glucose tolerance (IGT) receiving lifestyle intervention based on that used in the Diabetes Prevention Prog... | Older people; Biopsy; Blood Pressure; Body Mass Index; Cholesterol; Diabetic Diet | 2006-06-29 |
| 53 |
 |
Petti, Cathy A.; Polage, Christopher R. | Assessment of the utility of viral culture of cerebrospinal fluid | Nucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species... | Enterovirus Infections; Herpesviridae Infections; Virus Cultivation | 2006-12-15 |
| 54 |
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Dwight, Zachary Lawrence | uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application | uMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono... | | 2011 |
| 55 |
 |
Roberts, William L. | Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principles | Standardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances. | | 2012 |
| 56 |
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Dwight, Zachary Lawrence | Laboratory driven, lean-to-adaptive prototyping in Parallel for Web software project identification and application development in health science research | Clinical research laboratories, bioinformatics core facilities, and health science organizations often rely on heavy planning based software development models to propose, build, and distribute software as a consumable product. Projects in non-agile software life cycles tend to have rigid ?plan-desi... | | 2012 |
| 57 |
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Dwight, Zachary Lawrence | uAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictions | Abstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce... | | 2012-01-01 |
| 58 |
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Litwin, Christine M. | Anti-tuberculosis IgG antibodies as a marker of active mycobacterium tuberculosis disease | Anti-Mycobacterium tuberculosis IgG antibodies may aid in the diagnosis of active M. tuberculosis disease. We studied whether anti-M. tuberculosis IgG antibodies are elevated in active M. tuberculosis disease and assessed factors contributing to false positive and negative results. A retrospective s... | | 2012-01-01 |
| 59 |
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Wittwer, Carl T. | Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments | BACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS: A continuous assay that monitors DNA polymerase extension us... | | 2014-01-01 |
| 60 |
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Dwight, Zachary Lawrence | Heterozygote PCR product melting curve prediction | Melting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four... | | 2014-01-01 |
| 61 |
 |
Planelles, Vicente | Kinase control of latent HIV-1 infection: PIM-1 kinase as a major contributor to HIV-1 reactivation | Despite the clinical relevance of latent HIV-1 infection as a block to HIV-1 eradication, the molecular biology of HIV-1 latency remains incompletely understood. We recently demonstrated the presence of a gatekeeper kinase function that controls latent HIV-1 infection. Using kinase array analysis w... | | 2014-01-01 |
| 62 |
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Wittwer, Carl T. | Genotyping accuracy of high-resolution DNA melting instruments | High-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assesse... | | 2014-01-01 |
| 63 |
 |
Rockwood, Alan L. | Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometry | Background: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show... | | 2014-01-01 |
