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51 Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M.Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysisDifferentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar...MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR2006-01-09
52 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) and COBAS HCV TaqMan ASR Assays using the Vidiera NSP sample preparation instrument.The Vidiera Nsp nucleic sample preparation instrument (Vidiera,Beckman-Coulter Inc., Fullerton CA) was evaluated using the COBAS HBV TaqMan Analyte Specific Reagent (ASR. Roche Molecular Diagnostics [RMD], Indianapolis, IN) and COBAS HCV TaqMan ASR (RMD) assays using clinical samples and HCV Armored...Quantitative Tests; Linearity Studies2006-05-05
53 Smith, A. Gordon; Singleton, J. RobinsonLifestyle intervention for pre-diabetic neuropathy.OBJECTIVE: The purpose of this study was to evaluate intraepidermal nerve fiber density (IENFD) as a sensitive measure of neuropathy change in patients with neuropathy associated with impaired glucose tolerance (IGT) receiving lifestyle intervention based on that used in the Diabetes Prevention Prog...Aged; Biopsy; Blood Pressure; Body Mass Index; Cholesterol; Diabetic Diet2006-06-29
54 Petti, Cathy A.; Polage, Christopher R.Assessment of the utility of viral culture of cerebrospinal fluidNucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species...Enterovirus Infections; Herpesviridae Infections; Virus Cultivation2006-12-15
55 Dwight, Zachary LawrenceuMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web applicationuMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono...2011
56 Roberts, William L.Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principlesStandardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances.2012
57 Dwight, Zachary LawrenceLaboratory driven, lean-to-adaptive prototyping in Parallel for Web software project identification and application development in health science researchClinical research laboratories, bioinformatics core facilities, and health science organizations often rely on heavy planning based software development models to propose, build, and distribute software as a consumable product. Projects in non-agile software life cycles tend to have rigid ?plan-desi...2012
58 Litwin, Christine M.Anti-tuberculosis IgG antibodies as a marker of active mycobacterium tuberculosis diseaseAnti-Mycobacterium tuberculosis IgG antibodies may aid in the diagnosis of active M. tuberculosis disease. We studied whether anti-M. tuberculosis IgG antibodies are elevated in active M. tuberculosis disease and assessed factors contributing to false positive and negative results. A retrospective s...2012-01-01
59 Dwight, Zachary LawrenceuAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictionsAbstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce...2012-01-01
60 Dwight, Zachary LawrenceHeterozygote PCR product melting curve predictionMelting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four...2014-01-01
61 Rockwood, Alan L.Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometryBackground: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show...2014-01-01
62 Wittwer, Carl T.Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instrumentsBACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS: A continuous assay that monitors DNA polymerase extension us...2014-01-01
63 Planelles, VicenteKinase control of latent HIV-1 infection: PIM-1 kinase as a major contributor to HIV-1 reactivationDespite the clinical relevance of latent HIV-1 infection as a block to HIV-1 eradication, the molecular biology of HIV-1 latency remains incompletely understood. We recently demonstrated the presence of a gatekeeper kinase function that controls latent HIV-1 infection. Using kinase array analysis w...2014-01-01
64 Wittwer, Carl T.Genotyping accuracy of high-resolution DNA melting instrumentsHigh-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assesse...2014-01-01
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