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Creator | Title | Description | Subject | Date |
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Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V. | Cross platform comparison of melting curve analysis | Melting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt... | PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping | 2005-11-04 |
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Jaskowski, Troy D. | Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responses | The Author's have developed a simple, nested polymerase chain reaction (PCR) assay for amplification of an outer surface protein A (OspA) gene fragment of Borrelia burgdorferi using rapid temperature cycling and ethidium bromide detection on agarose gels, and applied it to the diagnosis of Lyme dis... | PCR; Rapid cycle amplification | 1996 |
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Jayamohan, Harikrishnan | Advances in microfluidics and lab on a chip technologies | Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains like biology research, infectious disease management, food safety and bio-defense. The emergence of microfluidics and nanotechnologies has enabled both new capabilities and instrument sizes ... | Microfluidics; Lab on a chip; Point of care; PCR; Isothermal amplification; High resolution melting analysis; Cytogenetics; Protein detection; Next generation sequencing; Sample preparation; Library preparation; Cell sorting | 2017 |