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Filters: Collection: "ir_uspace" Subject: "Molecular Sequence Data"
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CreatorTitleDescriptionSubjectDate
1 Baehr, WolfgangHuman interstitial retinoid-binding protein. Gene structure and primary structureInterstitial retinoid-binding protein (IRBP) is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix and is known to bind retinoids and fatty acids. We have used cDNA clones encoding human IRBP to isolate a 15-kilobase genomic fragment that encompasses the complete ...Molecular Sequence Data; Eye Proteins; Retinol-Binding Proteins; Messenger RNA1989-05-15
2 Zhang, KangMolecular and developmental characterization of the heat shock cognate 4 gene of Drosophila melanogasterThe Drosophila heat shock cognate gene 4 (hsc4), a member of the hsp70 gene family, encodes an abundant protein, hsc70, that is more similar to the constitutively expressed human protein than the Drosophila heat-inducible hsp70. Developmental expression revealed that hsc4 transcripts are enriched in...Embryo, Nonmammalian; Molecular Sequence Data1990
3 Baehr, WolfgangIn vitro isoprenylation and membrane association of mouse rod photoreceptor cGMP phosphodiesterase alpha and beta subunits expressed in bacteriaWe investigated the specificity of CAAX box-related isoprenylation of rod photoreceptor cGMP phosphodiesterase (PDE) subunits expressed in bacteria and the consequences of this modification on rod disk membrane association. Full-length cDNA sequences of the alpha and beta subunits of mouse PDE, inse...Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Molecular Sequence Data1992
4 Baehr, WolfgangCharacterization of the chicken GCAP gene array and analyses of GCAP1, GCAP2, and GC1 gene expression in normal and rd chicken pinealPURPOSE: This study had three objectives: (1) to characterize the structures of the chicken GCAP1 and GCAP2 genes; (2) to determine if GCAP1, GCAP2, and GC1 genes are expressed in chicken pineal gland; (3) if GC1 is expressed in chicken pineal, to determine if the GC1 null mutation carried by the re...Molecular Sequence Data; Promoter Regions (Genetics); Retinal Degeneration1999
5 Baehr, WolfgangGuanylyl cyclase activating protein. A calcium-sensitive regulator of phototransductionGuanylyl cyclase activating protein (GCAP1) has been proposed to act as a calcium-dependent regulator of retinal photoreceptor guanylyl cyclase (GC) activity. Using immunocytochemical and biochemical methods, we show here that GCAP1 is present in rod and cone photoreceptor outer segments where photo...Cloning, Molecular; DNA Primers; Molecular Sequence Data1995
6 Liew, Michael; Erali, Maria; Page, Sam; Hillyard, David R.; Wittwer, Carl T.Hepatitis C genotyping by denaturing high-performance liquid chromatographyDetermination of the hepatitis C virus (HCV) genotype for infected patients increasingly has become accepted as the standard of care. Genotype assignment helps in assessing disease prognosis and assists in establishing the appropriate duration of treatment. The great genetic diversity of HCV, with 1...Base Sequence; 5' Untranslated Regions; Genotype; Hepacivirus; Molecular Sequence Data2004-01
7 Poulter, Charles DaleEnzymes encoded by the farnesyl diphosphate synthase gene family in the big sagebrush Artemisia tridentata ssp. spiciformisFarnesyl diphosphate synthase catalyzes the sequential head-to-tail condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate. In plants the presence of farnesyl diphosphate synthase isozymes offers the possibility of differential regulation. Three full-length cDNAs enc...Amino Acid Sequence; Base Sequence; Molecular Sequence Data2003-08-22
8 Gesteland, Raymond F.; Atkins, John F.; Weiss, Robert B.Slippery runs, shifty stops, backward steps and forward hops: -2, -1, +1, +2, +5 and +6 Ribosomal frameshiftingFrameshift mutations frequently express residual levels of gene activity; that is, they are often leaky. This leakiness can be used as a tool to define the functional components that affect the reading frame during gene expression. Recent technological advances in the capability to efficiently bu...Molecular Sequence Data; Mutation; RNA, Transfer; Frameshifting1987
9 Thomas, Kirk R.; Capecchi, Mario R.Location of crossovers during gene targeting with insertion and replacement vectors.Gene targeting was used to introduce nonselectable genetic changes into chromosomal loci in mouse embryo-derived stem cells. The nonselectable markers were linked to a selectable marker in both insertion- and replacement-type vectors, and the transfer of the two elements to the Hprt locus was assaye...Genetic Vectors; Molecular Sequence Data; Restriction Mapping1993-04
10 Baehr, Wolfgang; Zhang, KangElovl4 mRNA distribution in the developing mouse retina and phylogenetic conservation of Elovl4 genesPURPOSE: Stargardt-like macular dystrophy (STGD3) is an autosomal dominant form of early onset macular degeneration. The disease causing gene ELOVL4 encodes a protein that belongs to a family of proteins functioning in elongation of long chain fatty acids. The purpose of this study is to characteriz...Amino Acid Sequence; Cloning, Molecular; Molecular Sequence Data2003
11 Baehr, WolfgangPharmacological and rAAV gene therapy rescue of visual functions in a blind mouse model of Leber congenital amaurosisBACKGROUND: Leber congenital amaurosis (LCA), a heterogeneous early-onset retinal dystrophy, accounts for approximately 15% of inherited congenital blindness. One cause of LCA is loss of the enzyme lecithin:retinol acyl transferase (LRAT), which is required for regeneration of the visual photopigmen...Disease Models, Animal; Genetic Vectors; Molecular Sequence Data2005
12 Baehr, Wolfgang; Zhang, Kang; Kolb, HelgaGene array and expression of mouse retina guanylate cyclase activating proteins 1 and 2PURPOSE: To identify gene arrangement, chromosomal localization, and expression pattern of mouse guanylate cyclase activating proteins GCAP1 and GCAP2, retina-specific Ca2+-binding proteins, and photoreceptor guanylate cyclase activators. METHODS: The GCAP1 and GCAP2 genes were cloned from genomic l...Molecular Sequence Data; Guanylate Cyclase-Activating Proteins; In Situ Hybridization, Fluorescence1998
13 Baehr, WolfgangIdentification and light-dependent translocation of a cone-specific antigen, (Cone Arrestin) recognized by monoclonal antibody 7G6PURPOSE: To elucidate the antigen recognized by monoclonal antibody (mAb) 7G6, a widely used cone-specific marker. METHODS: 7G6 immunocytochemistry was performed on sections of human, primate, and bovine retina. The antigen was immunoprecipitated from human retinal lysates and purified with protein ...Cones (Retina); Molecular Sequence Data; Phototransduction2003
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