TO
1 - 25 of 3
| Creator | Title | Description | Subject | Date | ||
|---|---|---|---|---|---|---|
| 1 |
 | Baehr, Wolfgang | Expression and mutagenesis of mouse rod photoreceptor cGMP phosphodiesterase | Using recombinant baculovirus vectors, the three subunits of mouse rod photoreceptor cGMP phosphodiesterase (PDE) (alpha beta gamma 2) have been expressed in insect cells. The recombinant alpha,beta subunits accumulate to 5 mg/liter culture, but most (98%) of the expressed polypeptides are insoluble... | Electrophoresis, Polyacrylamide Gel; Macromolecular Substances; Protein Processing, Post-Translational | 1994 |
| 2 |
 | Baehr, Wolfgang | Characterization of bovine rod outer segment G-protein | A simple modified procedure is described for isolating and purifying peripherally bound membrane proteins from bovine rod outer segment disks. The methods yield milligram quantities of G-protein and cGMP phosphodiesterase which are suitable for reconstitution with membranes containing visual pi... | Heterotrimeric GTP-Binding Proteins; Macromolecular Substances; Rhodopsin | 1982 |
| 3 |
 | Baehr, Wolfgang | Isolation and characterization of cGMP phosphodiesterase from bovine rod outer segments | A simple purification method has been developed for isolation of bovine cGMP phosphodiesterase from photoreceptor rod outer segments. The enzyme is peripherally membrane bound in its native state and is present in relatively high concentrations. In the bovine photoreceptors its molar ratio to rhodop... | Enzyme Activation; Macromolecular Substances; Trypsin/pharmacology | 1979 |
1 - 25 of 3