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CreatorTitleDescriptionSubjectDate
1 Gesteland, Raymond F.; Ives, Jeffrey T.; Stockham, Thomas G.Automated film reader for DNA sequencing based on homomorphic deconvolutionAn automated reader for electrophoresis based DNA sequencing methods is described that provides fast and accurate sequence determination. Digitized sequencing lanes are processed with homomorphic blind deconvolution in preparation for peak detection, interlane alignment, peak refinement and base cal...Sequence Analysis; Electrophoresis; Automated Film Reader; Homomorphic Deconvolution1994
2 Gesteland, Raymond F.; Atkins, John F.; Tuohy, Therese M.Uninterrupted translation through putative 12-nucleotide coding gap in sequence of carA: business as usualPrevious work of others reported an untranslated stretch of 12 nucleotides in the 5' coding sequence of carA from Pseudomonas aeruginosa. However, N-terminal protein sequencing of carA-lacZ translational fusions shows that these 12 nucleotides are normally translated in a continuous triplet manner, ...Base Sequence; Protein Biosynthesis; Sequence Deletion1994
3 Gesteland, Raymond F.Processing of adenovirus 2-induced proteinsAnalysis of (35)S-methionine-labeled extracts of adenovirus 2-infected KB cells revealed 22 virus-induced polypeptide components. Most proteins of the virion were easily detected in extracts of whole cells labeled for short periods between 15 and 30 h after infection; however, several virion compone...Adenoviridae; Viral Proteins; Protein Precursors; Methionine; Mouth Neoplasms1973
4 Hendee, Shonn P.; Faour, Fouad A.; Christensen, Douglas A.; Patrick, Baharah; Durney, Carl H.; Blumenthal, Donald K.Effects of weak extremely low frequency magnetic fields on calcium/calmodulin interactions.Mechanisms by which weak electromagnetic fields may affect biological systems are of current interest because of their potential health effects. Lednev has proposed an ion parametric resonance hypothesis (Lednev, 1991, Bioelectromagnetics, 12:71-75), which predicts that when the ac, frequency of a c...Electromagnetic Fields; Calcium-binding Proteins; Lednev's Theory1996-06
5 Gesteland, Raymond F.; Atkins, John F.; Weiss, Robert B.Slippery runs, shifty stops, backward steps and forward hops: -2, -1, +1, +2, +5 and +6 Ribosomal frameshiftingFrameshift mutations frequently express residual levels of gene activity; that is, they are often leaky. This leakiness can be used as a tool to define the functional components that affect the reading frame during gene expression. Recent technological advances in the capability to efficiently bu...Molecular Sequence Data; Mutation; RNA, Transfer; Frameshifting1987
6 Gesteland, Raymond F.; Weiss, Robert B.Efficient shine- Dalgarno sequence but not translation is necessary for LacZ mRNA stability in Escherichia coliThe 5' ends of many bacterial transcripts are important in determining mRNA stability. A series of Shine-Dalgarno (SD) sequence changes showed that the complementarity of the SD sequence to the anti-SD sequence of 16S rRNA correlates with lacZ mRNA stability in Escherichia coli. Several initiation c...Protein Biosynthesis; Ribosomes; Escherichia coli; Shine-Dalgarno Sequence1994
7 Gesteland, Raymond F.; Atkins, John F.Frameshifting in gene 10 of bacteriophage T7Gene 10 of bacteriophage T7, which encodes the most abundant capsid protein, has two products: a major product, 10A (36 kDa), and a minor product, 10B (41 kDa). 10B is produced by frameshifting into the -1 frame near the end of the 10A coding frame and is incorporated into the capsid. The frameshift...Frameshifting; Bacteriophage T7; RNA, Viral; Gene Expression1991
8 Angelucci, Alessandra; Lund, Jennifer S.Circuits for local and global signal integration in primary visual cortexContrast-dependent changes in spatial summation and contextual modulation of primary visual cortex (V1) neuron responses to stimulation of their receptive field reveal long-distance integration of visual signals within V1, well beyond the classical receptive field (cRF) of single neurons. To identif...Primary Visual Cortex; Extrastriate Cortex; Feed-back Connections; Lateral Connections; SUrround Modulation; Macaque2002-10-01
9 Normann, Richard A.; Campbell, Patrick K.; Jones, Kelly E.; Huber, Robert J.; Horch, Kenneth W.Silicon-based, three-dimensional neural interface: manufacturing processes for an intracortical electrode arrayA method has been developed for the manufacture of a "three-dimensional" electrode array geometry for chronic intracortical stimulation. This silicon based array consists of a 4.2 x 4.2 x 0.12 mm thick monocrystalline substrate, from which project 100 conductive, silicon needles sharpened to facilit...Electric Stimulation; Electrodes, Implanted; Sensory Cortex; Intracortical Electrode Array1991
10 Gesteland, Raymond F.; Atkins, John F.Evolutionary specialization of recoding: frameshifting in the expression of S. cerevisiae antizyme mRNA is via an atypical antizyme shift site but is still +1An autoregulatory translational shift to the +1 frame is required for the expression of ornithine decarboxylase antizyme from fungi to mammals. In most eukaryotes, including all vertebrates and a majority of the studied fungi/yeast, the site on antizyme mRNA where the shift occurs is UCC-UGA. The me...Antizyme; ODC; Polyamines; Frameshifting2006
11 Normann, Richard A.Effects of GABA and related drugs on horizontal cells in the isolated turtle retinaThe role of GABA in the outer plexiform layer of the turtle retina has been examined by intracellular recordings from L- and C-type horizontal cells in the isolated retina preparation. GABA (1-5 mM) slightly depolarized the L-type horizontal cells, reduced the amplitude of their photoresponses, and ...Retina; Horizontal Cells; Synapse; Negative Feedback; GABA; Turtle1990
12 Gesteland, Raymond F.; Atkins, John F.; Baranov, Pavel V.P-site tRNA is a crucial initiator of ribosomal frameshiftingThe expression of some genes requires a high proportion of ribosomes to shift at a specific site into one of the two alternative frames. This utilized frameshifting provides a unique tool for studying reading frame control. Peptidyl-tRNA slippage has been invoked to explain many cases of programmed ...Translation; Recoding; Kinetic Model; Frameshifting; Ribosome2004
13 Normann, Richard A.; Abbasi, Masoud; Johansson,TorbjornSilicon carbide enhanced thermomigrationThe widespread acceptance of thermomigration technology to produce through-chip interconnects has been impaired by (i) a random walk of the Si-Al liquid eutectic inclusion as it traverses the wafer, and (ii) a ?surface barrier? which allows thermomigration of only relatively large inclusions. In ...Silicon Dioxide; Thermometers; Transducers; Thermomigration Technology; Infrared Lamps1992
14 Normann, Richard A.; Muller, Jay F.; Kolb, HelgaSynaptic inputs to physiologically defined turtle retinal ganglion cellsTwo physiologically distinct, HRP-marked turtle retinal ganglion cells were examined for their morphology, GABAergic, glycinergic, and bipolar cell synaptic inputs, using electron-microscopic autoradiography and postembedding immunocytochemistry. One cell was a color-opponent, transient ON/OFF gangl...Turtles; Neural Pathways; Synapes; Retinal Ganglion Cells1991
15 Gesteland, Raymond F.Simian virus 40-specific polypeptides in Ad2+ND4- infected cellsA comparison of the proteins synthesized in human cells at late times after infection with adenovirus (Ad2) and with the adeno-simian virus 40 (SV40) hybrid viruses revealed polypeptides of 30,000 and 92,000 molecular weight specific for the hybrid viruses Ad2+ND1 and Ad2+ND4, respectively. Cell-fre...Molecular Weight; Peptide Biosynthesis; RNA, Messenger; Viral Proteins1976
16 Normann, Richard A.; Clark, Gregory A.; McDonnall, DanielSelective motor unit recruitment via intrafascicular multielectrode stimulationRecruitment of force via independent asynchronous firing of large numbers of motor units produces the grace and endurance of physiological motion. We have investigated the possibility of reproducing this physiological recruitment strategy by determining the selectivity of access to large numbers of ...Funtional Neuromuscular Stimulation; Microelectrode Array; Neuroprosthesis; Intrafascicular Multielectrode Stimulation; Cats2004
17 Normann, Richard A.; Campbell, Patrick K.; Jones, Kelly E.100 electrode intracortical array: structural variabilityA technique has been developed for fabricating three dimensional "hair brush" electrode arrays from monocrystalline silicon blocks. Arrays consist of a square pattern of 100 penetrating electrodes, with 400 microns interelectrode spacing. Each electrode is 1.5mm in length and tapers from about 100 m...Microelectrode arrays; Stimulation; Micromachining1990
18 Normann, Richard A.; Shoham, ShyCoding of position by simultaneously recorded sensory neurones in the cat dorsal root ganglionMuscle, cutaneous and joint afferents continuously signal information about the position and movement of individual joints. How does the nervous system extract more global information, for example about the position of the foot in space? To study this question we used microelectrode arrays to record...Cats; Sensory Neurones; Movement; Limb Position2004
19 Normann, Richard A.Control of retinal sensitivity. I. Light and dark adaptation of vertebrate rods and conesRods and cones in Necturus respond with graded hyperpolarization to test flashes spanning about 3.5 log units of intensity. Steady background levels hyperpolarize the rods, and the rod responses become progressively smaller as background level is increased. In cones, higher background levels reduce...Retina;Light and Dark Adaptation; Recepters; Bipolars; Ganglion Cells1974
20 Normann, Richard A.; Shoham, ShyEncoding mechanisms for sensory neurons studied with a multielectrode array in the cat dorsal root ganglionRecent advances in microelectrode array technology now permit a direct examination of the way populations of sensory neurons encode information about a limb's position in space. To address this issue, we recorded nerve impulses from about 100 single units simultaneously in the L6 and L7 dorsal root ...Sensory; Encoding; Multielectrode; Dorsal Root Ganglion; Cutaneour; Muscle2004
21 Normann, Richard A.; Kolb, Helga; Lasater, Eric M.Signal integration at the pedicle of turtle cone photoreceptors: an anatomical and electrophysiological studyThe morphology of the axon which connects the cell body and pedicle of turtle cone photoreceptors was studied by light and electron microscopy. The axon which contains numerous synaptic vesicles, some endoplasmic reticulum, and a few cisternae is basically filled with cytoplasm. The length of the ax...Cone Photoreceptor, Voltage Clamp; Isolated Cells; Input Resistance; Cell Body-Pedicle Coupling1989
22 Normann, Richard A.; Maynard, Edwin M.Neuronal interactions improve cortical population coding of movement directionInteractions among groups of neurons in primary motor cortex (MI) may convey information about motor behavior. We investigated the information carried by interactions in MI of macaque monkeys using a novel multielectrode array to record simultaneously from 12-16 neurons during an arm-reaching task. ...Motor Cortex; Monkey; Population Coding; Movement1999
23 Normann, Richard A.Effects of background illumination on the photoresponses of red and green cones1. The photoresponses of light- and dark-adapted red and green cone photoreceptors were recorded intracellularly in the retina of the turtle, Pseduemys scripta elegans. Background illumination produced similar effects on both types of cones. 2. In response to the onset of a prolonged, steady backgro...Turtles; Adaptation, Ocular; Retina; Signal Transmission1979
24 Normann, Richard A.; Horch, Kenneth W.; Cha, KichulMobility performance with a pixelized vision systemA visual prosthesis, based on electrical stimulation of the visual cortex, has been suggested as a means for partially restoring functional vision in the blind. The prosthesis would create a pixelized visual sense consisting of punctate spots of light (phosphenes). The present study investigated the...Visual Prosthesis; Mobility; Phosphene Simulator1992
25 Gesteland, Raymond F.Translation of Rl7 RNA fragmentsExamination of the events during infection of cells by RNA phages reveals phemonomena that are surprisingly complex for a virus that has only enough information to code for three to four proteins. The coat protein is synthesized at a rapid rate through most of the infectious cycle making it the pre...Electrophoresis; RNA, Messenger; Peptide Biosynthesis; Kinetics1969
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