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Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T. | Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis | This poster describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the method... | Genotyping; Amplicon Melting | 2005-06 |
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