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CreatorTitleDescriptionSubjectDate
126 Capecchi, Mario R.; Thomas, Kirk R.High frequency targeting of genes to specific sites in the mammalian genomeWe corrected a defective gene residing in the chromosome of a mammalian cell by injecting into the nucleus copies of the same gene carrying a different mutation. We determined how the number, the arrangement, and the chromosomal position of the integrated gene, as well as the number of injected mo...Homologous recombination; Mutant genes; Mammalian genome1986
127 Capecchi, Mario R.; Thomas, Kirk R.Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cellsWe mutated, by gene targeting, the endogenous hypoxanthine phosphorlbosyl transferase (HPFlT) gene in mouse embryo-derived stem (ES) cells. A specialized construct of the neomycin resistance (NO') gene was introduced into an exon of a cloned fragment of the Hprf gene and used to transfect ES cell...Site-directed mutagenesis; Hypoxanthine phosphorlbosyl transferase gene; HPFlT gene; Neomycin resistance gene; Homologous recombination; Mouse stem cells; Mouse embryo-derived stem cells1987
128 Beckerle, Mary C.Biochemical and molecular characterization of the chicken cysteine-rich protein, a developmentally regulated LIM-domain protein that is associated with the actin cytoskeletonLIM domains are present in a number of proteins including transcription factors, a protooncogene product, and the adhesion plaque protein zyxin. The LIM domain exhibits a characteristic arrangement of cysteine and histidine residues and represents a novel zinc binding sequence (Michelsen et al., ...Zyxin; Cysteine-rich proteins; cCRP; Actin; LIM domains1994
129 Davidson, Diane W.Competition and the structure of granivore communitiesWe trace the development of our investigations of granivory in desert ecosystems, illustrating the synthesis of the comparative and experimental approaches and noting the essential contributions of both. In the process, we also call attention to several major difficulties inherent to experimentatio...Granivory; Competition; Desert Ecosystems; Competitive investigations; Experimental investigations1980
130 Capecchi, Mario R.Hox10 and Hox11 genes are required to globally pattern the mammalian skeleton.Mice in which all members of the Hox10 or Hox11 paralogous group are disrupted provide evidence that these Hox genes are involved in global patterning of the axial and appendicular skeleton. In the absence of Hox10 function, no lumbar vertebrae are formed. Instead, ribs project from all posterior ve...Alleles; Animals; Forelimb; Gene Expression Regulation, Developmental; Hindlimb2003-07-18
131 Golden, Kenneth M.Transition in the fractal geometry of Arctic melt pondsDuring the Arctic melt season, the sea ice surface undergoes a remarkable transformation from vast expanses of snow covered ice to complex mosaics of ice and melt ponds. Sea ice albedo, a key parameter in climate modeling, is determined by the complex evolution of melt pond configurations. In fact, ...2012-01-01
132 Clayton, Dale H.An experimental test of the effects of behavioral and immunological defenses against vectors: do they interact to protect birds from blood parasites?Background: Blood-feeding arthropods can harm their hosts in many ways, such as through direct tissue damage and anemia, but also by distracting hosts from foraging or watching for predators. Blood-borne pathogens transmitted by arthropods can further harm the host. Thus, effective behavioral and im...2014-01-01
133 Gesteland, Raymond F.; Ives, Jeffrey T.; Stockham, Thomas G.Automated film reader for DNA sequencing based on homomorphic deconvolutionAn automated reader for electrophoresis based DNA sequencing methods is described that provides fast and accurate sequence determination. Digitized sequencing lanes are processed with homomorphic blind deconvolution in preparation for peak detection, interlane alignment, peak refinement and base cal...Sequence Analysis; Electrophoresis; Automated Film Reader; Homomorphic Deconvolution1994
134 Bowling, David R.Ecological processes dominate the 13C land disequilibrium in a Rocky Mountain subalpine forestFossil fuel combustion has increased atmospheric CO2 by ≈ 115 μmol mol1 since 1750 and decreased its carbon isotope composition (δ13C) by 1.7-2‰(the 13C Suess effect). Because carbon is stored in the terrestrial biosphere for decades and longer, the δ13C of CO2 released by terrestrial ecosyst...2014-01-01
135 Jorgensen, ErikCaenorhabditis elegans gene unc-25 encodes glutamic acid decarboxylase and is required for synaptic transmission but not synaptic developmentThe neurotransmitter GABA has been proposed to play a role during nervous system development. We show that the Caenorhabditis elegans gene unc-25 encodes glutamic acid decarboxylase (GAD), the GABA biosynthetic enzyme. unc-25 is expressed specifically in GABAergic neurons. Null mutations in unc-25...1999
136 Beckerle, Mary C.Cysteine-rich protein family of highly related LIM domain proteinsHere we describe a family of closely related LIM domain proteins in avian cells. The LIM motif defines a zinc-binding domain that is found in a variety of transcriptional regulators, proto-oncogene products, and proteins associated with sites of cell-substratum contact. One type of LIM-domain protei...LIM domains; Chicken embryo fibroblasts; Quail embryo fibroblasts; Cell growth; Cell development; Cysteine-rich proteins1995
137 Olivera, Baldomero M.Identification of a novel pharmacophore for peptide toxins interacting with K+ channelsқM-conotoxin RIIIK blocks TSha1 K+ channels from trout with high affinity by interacting with the ion channel pore. As opposed to many other peptides targeting K+ channels, қM-RIIIK does not possess a functional dyad. In this study we combine thermodynamic mutant cycle analysis and docking calc...Conotoxins; Peptide toxins; қM-conotoxin RIIIK; Pharmacophore2005
138 Capecchi, Mario R.Removing the vertebrate-specific TBP N terminus disrupts placental beta2m-dependent interactions with the maternal immune systemMammalian TBP consists of a 180 amino acid core that is common to all eukaryotes, fused to a vertebrate-specific N-terminal domain. We generated mice having a modified tbp allele, tbp(DeltaN), that produces a version of TBP lacking 111 of the 135 vertebrate-specific amino acids. Most tbp(DeltaN/Delt...Alleles; Animals, Genetically Modified; Binding Sites; Embryonic and Fetal Development; Evolution, Molecular; Female; Fetus; Immune Tolerance; Male; Mice; Mutation; ATA-Box Binding Protein2002-07-12
139 Coley, Phyllis D.Benefits and costs of defense in a neotropical shrubBenefits and costs are central to optimality theories of plant defense. Benefit is the gain in fitness to reducing herbivory and cost is the loss in fitness to committing resources to defense. We evaluate the benefits and costs of defense in a neotropical shrub, Psychotria horizontalis. Plants were ...Cost of defense; Growth-defense trade-off; Exclosures; Field experiment; Herbivory; Panama; Psychotria horizontalis; Rubiaceae; Tannins; Toughness; Tropics1995
140 Olivera, Baldomero M.Alternative splicing in the pore-forming region of shaker potassium channelsWe have cloned cDNAs for the shaker potassium channel gene from the spiny lobster Panulirus interruptus. As previously found in Drosophila, there is alternative splicing at the 59 and 39 ends of the coding region. However, in Panulirus shaker, alternative splicing also occurs within the pore-forming...Panulirus interruptus; Conotoxins; Shaker; Stomatogastric ganglion; Pore-forming region; Alternative splicing1997
141 Mattis, Daniel C.A contribution to the many-fermion problemIn this work we reexamine the many-fermion problem in arbitrary dimensions. It is shown that in two dimensions or higher, the Hamiltonian of interacting fermions can be separated into individual nonintersecting sectors labeled by the wave-vector q. Within each sector the Hamiltonian maps onto a gene...2012-01-01
142 Thomas, Kirk R.; Capecchi, Mario R.Introduction of homologous DNA sequences into mammalian cells induces mutations in the cognate gene.Injection of homologous DNA sequences into nuclei of cultured mammalian cells induces mutations in the cognate chromosomal gene. It appears that these mutations result from incorrect repair of a heteroduplex formed between the introduced and the chromosomal sequence. This phenomenon is termed 'heter...Animals; Cell Line; Drug Resistance, Microbial; Fibroblasts; Mice; Models, Genetic; Neomycin; Plasmids1986-11-06
143 Capecchi, Mario R.; Thomas, Kirk R.Swaying is a mutant allele of the proto-oncogene Wnt-1Mice homozygous for the recessive mutation swaying (SW) are characterized by ataxia and hypertonia, attributed to the malformation of anterior regions of the cerebellum. We show that SW is a deletion of a single base pair from the proto-oncogene Wnf-1. The deletion is predicted to cause premature t...Swaying allele; protooncogene Wnt-1; Mutant genes; Mutant alleles1991
144 Carrier, David R.Functional tradeoffs in specialization for fighting versus runningBoth locomotion and fighting are critical to survival and reproductive fitness in many vertebrate species. Yet, characters that make an individual good at fighting may, in many cases, limit locomotor performance and vice versa. Here I describe tests of three functional tradeoffs in the limb muscles ...Greyhounds; Pit Bulls; Australopithecus; Homo; Fighting; Running; Male-male aggression; Limb muscles; Functional tradeoff2002
145 Coley, Phyllis D.Beneficios y costos de defensa en un arbusto del neotrópicoLOS beneficios y costos son de importancia capital para las teorfas de optimization de las defensas de las plantas. El beneficio es la ganancia de aptitud para reducir el herbivorismo; el costo es la perdida de aptitud al comprometer recursos en defensa. Evaluamos los beneficios y los costos de def...Psychotria horizontalis; Isla de Barro Colorado; Costos de defense; Celdas de exclusion; Herbivorismo; Defensa de las plantas; Taninos; Dureza; Tropicos; Benefit; Beneficios2007
146 Beckerle, Mary C.Members of the zyxin family of LIM proteins interact with members of the p130cas family of signal transducersIntegrin binding to extracellular matrix proteins induces formation of signaling complexes at focal adhesions. Zyxin co-localizes with integrins at sites of cellsubstratum adhesion and is postulated to serve as a docking site for the assembly of multimeric protein complexes involved in regulating ce...Zyxin; Actin; LIM domains; Trip6; Focal adhesions2002
147 Beckerle, Mary C.Mutations in Drosophila enabled and rescue by human vasodilator-stimulated phosphoprotein (VASP) indicate important functional roles for Ena/VASP homology domain 1 (EVH1) and EVH2 domainsDrosophila Enabled (Ena) was initially identified as a dominant genetic suppressor of mutations in the Abelson tyrosine kinase and, more recently, as a member of the Ena/human vasodilator-stimulated phosphoprotein (VASP) family of proteins. We have used genetic, biochemical, and cell biological appr...Drosophila Ena; VASP1998
148 Beckerle, Mary C.Zyxin and cCRP: Two interactive LIM domain proteins associated with the cytoskeletonInteraction with extracellular matrix can trigger a variety of responses by cells including changes in specific gene expression and cell differentiation. The mechanism by which cell surface events are coupled to the transcriptional machinery is not understood, however, proteins localized at sites o...Zyxin; Cysteine-rich proteins; cCRP; LIM domain1992
149 Thomas, Kirk R.; Capecchi, Mario R.Maintenance of functional equivalence during paralogous Hox gene evolution.Biological diversity is driven mainly by gene duplication followed by mutation and selection. This divergence in either regulatory or protein-coding sequences can result in quite different biological functions for even closely related genes. This concept is exemplified by the mammalian Hox gene comp...Alleles; Animals; Cervical Vertebrae; Embryo; Genetic Complementation Test; Homeodomain Proteins; Homozygote; Mice2000-02-10
150 Sperry, John S.Inter-tracheid pitting and the hydraulic efficiency of conifer wood: the role of tracheid allometry and cavitation protectionPlant xylem must balance efficient delivery of water to the canopy against protection from air entry into the conduits via air-seeding. We investigated the relationship between tracheid allometry, end wall pitting, safety from air-seeding, and the hydraulic efficiency of conifer wood in order to bet...Allometry; Araucariaceae; cavitation2006
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