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CreatorTitleDescriptionSubjectDate
1 Gesteland, Raymond F.; Atkins, John F.; Ingram, Jennifer A.; Kelly, Paul J.; Grentzmann, GuidoDual-luciferase reporter system for studying recoding signalsA new reporter system has been developed for measuring translation coupling efficiency of recoding mechanisms such as frameshifting or readthrough. A recoding test sequence is cloned in between the renilla and firefly luciferase reporter genes and the two luciferase activities are subsequently measu...Amino Acid Sequence; Genes, Reporter; HIV; Antizyme; Translation1998
2 Gesteland, Raymond F.; Atkins, John F.Influence of the stacking potential of the base 3' of tandem shift codons on -1 ribosomal frameshifting used for gene expressionTranslating ribosomes can shift reading frame at specific sites with high efficiency for gene expression purposes. The most common type of shift to the 1 frame involves a tandem realignment of two anticodons from pairing with mRNA sequence of the form X XXY YYZ to XXX YYY Z where the spaces indicate...1 frameshifting; 39 context effect; Codon anticodon interaction; Escherichia coli ; Recoding; tRNALys; XXXY YYZ frameshift motifs2002
3 Olivera, Baldomero M.On the mechanism of the polynucleotide joining reactionPolynucleotide-joining enzymes (ligases) have been identified in uninfected and phage-infected Escherichia coli (Gellert, 1967; Olivera and Lehman, 1967a; Zimmerman et al., 1967; Gefter et al., 1967; Weiss and Richardson, 1967a; Cozzarelli et al., 1967). Both types of enzyme catalyze the synthesis o...Enzyme-AMP; Enzyme-Adenylate; DNA-Adenylate; Poly dt-Adenylate; Pyrophosphate bond1968
4 Hughes, Kelly T.Flagellar-specific transcription factor, σ28, is the Type III secretion chaperone for the flagellar-specific anti-σ28 factor FlgMThe σ28 protein is a member of the bacterial σ70-family of transcription factors that directs RNA polymerase to flagellar late (class 3) promoters. The σ28 protein is regulated in response to flagellar assembly by the anti-σ28 factor FlgM. FlgM inhibits σ28-dependent transcription of genes wh...Flagellum; gene regulation; Type III secretion; FlgM2006
5 Hughes, Kelly T.Flagellar anti-σ factor FlgM actively dissociates Salmonella typhimurium σ28 RNA polymerase holoenzymeThe anti-σ factor FlgM of Salmonella typhimurium inhibits transcription of class 3 flagellar genes through a direct interaction with the flagellar-specific σ factor, σ28. FlgM is believed to prevent RNA polymerase (RNAP) holoenzyme formation by sequestering free σ28. We have analyzed FlgM-media...sigma-factors; transcription; FlgM; Flagellum1998
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