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![]() | Capecchi, Mario R. | Gene targeting: an historical perspective | Our entry into what was going to become the field of gene targeting began in 1977. I was experimenting with the use of extremely small glass needles to inject DNA directly into the nuclei of living cells. The needles were controlled by hydraulically driven micro-manipulators and were directed into n... | Gene targeting -- History; Homologous recombination | 1998 |