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| Creator | Title | Description | Subject | Date | ||
|---|---|---|---|---|---|---|
| 1 |
 | Thomas, Kirk R.; Capecchi, Mario R. | Efficient correction of mismatched bases in plasmid heteroduplexes injected into cultured mammalian cell nuclei. | Heteroduplexes were prepared from two plasmids, pRH4-14/TK and pRH5-8/TK, containing different amber mutations in the neomycin resistance gene (Neor). The Neor gene was engineered to be expressed in both bacterial and mammalian cells. A functional Neor gene conferred kanamycin resistance to bacteria... | Cell Nucleus; Cells, Cultured; Microinjections | 1985-01 |
| 2 |
 | Capecchi, Mario R. | High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. | Direct microinjection of DNA by glass micropipettes was used to introduce the Herpes simplex virus thymidine kinase gene into cultured mammalian cells. When DNA was delivered directly into the nuclei of LMTK-, a mouse cell line deficient in thymidine kinase activity, 50--100% of the cells expressed ... | Cell Nucleus; Cytoplasm; DNA, Viral; Microinjections; Recombination, Genetic | 1980-11-22 |
| 3 |
 | Capecchi, Mario R. | Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules. | We examined the fate of DNA microinjected into nuclei of cultured mammalian cells. The sequence composition and the physical form of the vector carrying the selectable gene affected the efficiency of DNA-mediated transformation. Introduction of sequences near the simian virus 40 origin of DNA replic... | Base Sequence; Cell Line; Genes, Viral; Genetic Vectors; Mice; Microinjections | 1982-11 |
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