Creator | Title | Description | Subject | Date | ||
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1 |
![]() | Thomas, Kirk R.; Capecchi, Mario R. | Nonreciprocal exchanges of information between DNA duplexes coinjected into mammalian. cell nuclei | We have examined the mechanism of homologous recombination between plasmid molecules coinjected into cultured mammalian cells. Cell lines containing recombinant DNA molecules were obtained by selecting for the reconstruction of a functional Neor gene from two plasmids that bear different amber mutat... | Animals; Cells, Cultured; DNA Restriction Enzymes; Kinetics | 1985-01 |
2 |
![]() | Capecchi, Mario R. | Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells. | We have used site-specific mutagenesis to change the anticodon of a Xenopus laevis tyrosine tRNA gene so that it would recognize ochre codons. This tRNA gene is expressed when amplified in monkey cells as part of a SV40 recombinant and efficiently suppresses termination at both the ochre codon separ... | Xenopus; Nucleic Acid Conformation; Kidney; DNA Restriction Enzymes | 1984-11 |