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| Creator | Title | Description | Subject | Date | ||
|---|---|---|---|---|---|---|
| 1 |
 | Capecchi, Mario R. | Establishment of mammalian cell lines containing multiple nonsense mutations and functional suppressor tRNA genes. | We describe the generation of mammalian cell lines carrying amber suppressor genes. Nonsense mutants in the herpes simplex virus thymidine kinase (HSV tk) gene, the Escherichia coli xanthine-guanine phosphoribosyl transferase (Eco-gpt) gene and the aminoglycoside 3' phosphotransferase gene of the Tn... | Cercopithecus aethiops; Escherichia coli; Xenopus | 1982-11-30 |
| 2 |
 | Capecchi, Mario R. | High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. | Direct microinjection of DNA by glass micropipettes was used to introduce the Herpes simplex virus thymidine kinase gene into cultured mammalian cells. When DNA was delivered directly into the nuclei of LMTK-, a mouse cell line deficient in thymidine kinase activity, 50--100% of the cells expressed ... | Cell Nucleus; Cytoplasm; DNA, Viral; Microinjections; Recombination, Genetic | 1980-11-22 |
| 3 |
 | Capecchi, Mario R. | Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA. | Biochemical transformation of thymidine-kinase-deficient (TK-) mouse L cells is enhanced 20 to 40 fold when microinjected plasmid DNA contains regions of the genomes of Rous sarcoma virus or simian virus 40 in addition to the complete herpes simplex virus tk gene, irrespective of the orientation and... | Animals; Base Sequence; Genes, Viral; Plasmids; Thymidine Kinase | 1983-07-01 |
| 4 |
 | Capecchi, Mario R. | Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro. | tRNA isolated from two different yeast super-suppressor strains translates a known nonsense mutation in vitro, whereas tRNA from a closely related nonsuppressing strain does not. Suppression was assayed by translation of RNA isolated from an amber coat mutant of bacteriophage Qbeta (GB11) in a prote... | Codon; Escherichia coli; Protein Biosynthesis | 1975-11 |
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