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1 Capecchi, Mario R.Gene targeting: an historical perspectiveOur entry into what was going to become the field of gene targeting began in 1977. I was experimenting with the use of extremely small glass needles to inject DNA directly into the nuclei of living cells. The needles were controlled by hydraulically driven micro-manipulators and were directed into n...Gene targeting -- History; Homologous recombination1998
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