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Creator | Title | Description | Subject | Date |
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Coffin, Cheryl M.; Capecchi, Mario R. | Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function. | Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has... | Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors | 2004-11-01 |
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Vaughn, Cecily P.; Crockett, David K.; Lim, Megan S.; Elenitoba-Johnson, Kojo S.J. | Analytical characteristics of ICAT-LC-MS/MS for quantitative proteomics studies | Recent studies have employed isotope-coded affinity tags (ICAT) in concert with tandem mass spectrometry (MS/MS) to assess differential expression of proteins on a proteomic scale and perform unbiased quantitative comparisons between samples from different physiological or disease states. We sought ... | isotope-coded affinity tags; ICAT; mass spectrometry; MS/MS; proteomic scale; FL | 2005-06-03 |
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Petti, Cathy A.; Polage, Christopher R. | Assessment of the utility of viral culture of cerebrospinal fluid | Nucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species... | Enterovirus Infections; Herpesviridae Infections; Virus Cultivation | 2006-12-15 |
| 4 |
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Digre, Kathleen B.; Gouw, Launce G.; Harris, Catherine P.; Haines, John H.; Ptacek, L.J. | Autosomal dominant cerebellar ataxia with retinal degeneration: clinical, neuropathologic, and genetic analysis of a large kindred. | The autosomal dominant cerebellar ataxias (ADCA) comprise a heterogeneous group of neurologic disorders characterized by degeneration of the cerebellum, spinal cord, and brainstem. Genetic analysis has revealed two loci, SCA1 on chromosome 6p, and SCA2 on chromosome 12q, responsible for some ADCA. W... | Retinal Degeneration; Cerebellar Ataxia; Genetic Analysis | 1994-08 |
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Digre, Kathleen B.; Blumenthal, Deborah T.; Cessna, Melissa H.; Gopez, Evelyn V.; Shen, Yue | Carcinomatous meningitis as the presenting manifestation of gallbladder carcinoma: case report and review of the literature | The primary tumors that typically cause carcinomatous meningitis include lung cancer, breast cancer, leukemia, lymphoma and melanoma. A variety of neurological signs and symptoms can be seen depending on the extent and location of the meningeal metastasis. Once the diagnosis of carcinomatous meningi... | Carcinomatous Meningitis; Gallbladder Carcinoma; Leptomeningeal Metastasis; Meningeal Carcinomatosis; Cranial Neuropathy | 2004-10 |
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Owen, William E.; Roberts, William L. | Comparable effects of DIGIBIND and DigiFab in thirteen digoxin immunoassays | Digoxin is widely prescribed for the treatment of cardiac conditions (1). Because of its narrow therapeutic range, digoxin-related toxicity resulting from acute or chronic overdose is common. Metabolites of digoxin as well as related compounds, including digitoxin, tanshinones, bufandienolide, a... | DIGIBIND; DigiFab; Digoxin immunoassays; Digoxin toxicity | 2002 |
| 7 |
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Jaskowski, Troy D. | Comparison of capillary zone and immunosubtraction with agarose gel and immunofixation electrophoresis for detecting and identifying monoclonal gammopathies | Capillary zone electrophoresis (CZE) and immunosubtraction electrophoresis (ISE) were evaluated for ability to detect and immunotype monoclonal proteins, compared with agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE), respectively. Six hundred seventeen serum samples wer... | Capillary zone electrophoresis; Immunosubtraction electrophoresis; CZE; ISE; Monoclonal protein electrophoresis; Serum protoin electrophoresis; Agarose gel; Dysproteinemias | 1999 |
| 8 |
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Owen, William E.; Roberts, William L. | Comparison of five automated serum and whole blood folate assays | Serum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonisotopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, wer... | Folate assays; Serum folate; Whole blood folate; Automated assays | 2003 |
| 9 |
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Jaskowski, Troy D. | Comparison of four enzyme immunoassays with a western blot assay for the determination of type-specific antibodies to herpes simplex virus | Most current enzyme immunoassays (EIAs) differentiate inadequately between types 1 and 2 herpes simplex virus (HSV) antibodies since significant crossreactivity exists. We compared 4 IgG type-specific EIAs using a Western blot assay for resolution of discrepant results. The Diamedix had sensitivit... | Enzyme immunoassays; Western blot assay; Type-specific antibodies | 2001 |
| 10 |
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Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R; Hillyard, David R | Comparison of the DIGENE HBV HYBRID CAPTURE II AND ROCHE COBAS Amplicor HBV Monitor Assays. | Evaluate and compare the performance characteristics of the COBAS Amplicor HBV Monitor test (Roche Diagnostics Corp.) and the HBV Hybrid capture II (Digene Corporation) assay for the quantification of HBV BNA Levels. | Quantitative Tests; Linearity Studies | 2001-08-19 |
| 11 |
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Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R.; Hillyard, David R. | Comparison of the National Genetics Institute (NGI) HCV Superquant and Roche COBAS Amplicor HCV Monitor, Version 2.0 Assays | Chronic Hepatitis C infection is currently recognized as an important health care problem. It is estimated that nearly 4 million Americans are infected, 15 to 20 percent of whom will eventually develop Cirrhosis. Current treatments for Hepatitis C virus (HCV) are limited to alfa-interferon alone or ... | Cirrhosis; Patients | 2000-04-22 |
| 12 |
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Kriesel, John D.; Baringer, J R; Spruance, Spotswood L. | Correlation between detection of herpes simplex virus in oral secretions by PCR and susceptibility to experimental UV radiation-induced herpes labialis. | We examined the oral secretions of 25 patients for herpes simplex virus (HSV) at the time of and following experimental UV radiation (UVR). HSV was detected in one or more oral secretion specimens in 5 of 12 (42%) cases by cell culture and in 8 of 12 (67%) cases by PCR. On the day of UVR, HSV was de... | Polymerase Chain Reaction; Ultraviolet Rays; Simplexvirus | 1994 |
| 13 |
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Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V. | Cross platform comparison of melting curve analysis | Melting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt... | PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping | 2005-11-04 |
| 14 |
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Owen, William E.; Roberts, William L. | Cross-reactivity of three recombinant insulin analogs with five commercial insulin immunoassays | Several new insulin analogs that are prepared with recombinant DNA technology are available for clinical use [for a recent review, see Ref. (1)]. These agents have altered pharmacokinetics compared with regular human insulin. Insulin aspart (Novo-LogTM; Novo Nordisk Pharmaceuticals) is homologous ... | Insulin analogs; Insulin immunoassays; Cross-reactivity | 2004 |
| 15 |
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Jaskowski, Troy D. | Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassay | We used a multiplexed fluorescent microsphere immunoassay to develop a sandwich capture assay to assess simultaneously the production of thymus helper (TH) 1- and TH2-type cytokines in tissue culture supernatant obtained from stimulated peripheral blood mononuclear cells. The assay then was use... | Multiplexed fluorescent microsphere immunoassay | 2002 |
| 16 |
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Owen, William E.; Rockwood, Alan L.; Roberts, William L.; Meikle, A. Wayne | Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids | Congenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17O... | Congenital adrenal hyperplasia; Adrenal steroids; Tandem mass spectrometry assay | 2006 |
| 17 |
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Cloud, Joann L.; Hoggan, Karen; Cohen, Samuel; Brown-Elliott, Barbara A.; Mann, Linda; Wilson, Rebecca; Aldous, Wade K.; Wallace, Jr, Richard J.; Woods, Gail L. | Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probes | This poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is ... | MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus | 2005-09-05 |
| 18 |
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Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M. | Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysis | Differentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar... | MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR | 2006-01-09 |
| 19 |
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Aldous, Jay A.; Engar, Richard C. | Do dentists prescribe narcotics excessively? | Dealing with pain is an inevitable sequela to dental treatment. Although several drug regimens primarily involving narcotics have been used in the past, availability of nonsteroidal anti-inflammatory drugs (NSAIDs) has increased recently. A study was conducted to analyze dental prescribing patterns ... | Analgesics, Opioid; Anti-Inflammatory Agents; Dentist's Practice Patterns; Pain, Postoperative | 1996-07-01 |
| 20 |
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Parker, Sam; Stevenson, Jeffery B.; Hillyard, David R. | Eclipse probe assay for the detection of mycoplasma pneumoniae. | Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. ... | Eclipse Hybridization Probe; Protocol; Techniques | 2005-09-11 |
| 21 |
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Normann, Richard A.; Chandler, John P. | Effects of calcium ions on L-type horizontal cells in the isolated turtle retina | A technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p... | Retina; Calcium; Horizontal Cell; Turtle | 1990 |
| 22 |
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Erickson, J. Alan; Aldeen, William E.; Ashwood, Edward R. | Evaluation of a fecal pancreatic elastase-1 enzyme-linked immunosorbent assay: assessment versus an established assay and implication in classifying pancreatic function | Disagreement continues regarding the two commercially available fecal pancreatic elastase-1 (PE-1) ELISAs and their respective capabilities to assess pancreatic function. Our objectives were to validate the newer PE-1 ELISA and evaluate the test against the previously established assay, to investiga... | PE-1; pancreatic elastase-1; FDA; AMR; analytical measurement range; BioServ Diagnostics PE-1ELISA; ScheBo Biotech PE-1ELISA | 2005-09-25 |
| 23 |
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Jaskowski, Troy D. | Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination control | Toxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction... | PCR probe capture assay; Uracil N-glycosylase; UNG | 2000 |
| 24 |
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Erickson, J. Alan; Ashwood, Edward R. | Evaluation of an enzyme-linked binding protein assay for hyaluronic acid and concentrations in hepatitis C infected patients | Serological hyaluronic acid (HA) has been proposed as a noninvasive alternative to liver biopsy for assessing the extent of liver fibrosis. Liver biopsy correctly identifies hepatic disease in about 65 to 75% of cases, being strongly dependent on the length of the biopsy obtained. Furthermore, hepat... | Hyaluronic Acid Concentration; Corgenix HA assay | 2005-07-21 |
| 25 |
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Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R. | Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay. | To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international... | Quantitative Tests; Linearity Studies | 2004-05-10 |
