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1 Coffin, Cheryl M.; Capecchi, Mario R.Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function.Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has...Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors2004-11-01
2 Vaughn, Cecily P.; Crockett, David K.; Lim, Megan S.; Elenitoba-Johnson, Kojo S.J.Analytical characteristics of ICAT-LC-MS/MS for quantitative proteomics studiesRecent studies have employed isotope-coded affinity tags (ICAT) in concert with tandem mass spectrometry (MS/MS) to assess differential expression of proteins on a proteomic scale and perform unbiased quantitative comparisons between samples from different physiological or disease states. We sought ...isotope-coded affinity tags; ICAT; mass spectrometry; MS/MS; proteomic scale; FL2005-06-03
3 Litwin, Christine M.Anti-tuberculosis IgG antibodies as a marker of active mycobacterium tuberculosis diseaseAnti-Mycobacterium tuberculosis IgG antibodies may aid in the diagnosis of active M. tuberculosis disease. We studied whether anti-M. tuberculosis IgG antibodies are elevated in active M. tuberculosis disease and assessed factors contributing to false positive and negative results. A retrospective s...2012-01-01
4 Petti, Cathy A.; Polage, Christopher R.Assessment of the utility of viral culture of cerebrospinal fluidNucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species...Enterovirus Infections; Herpesviridae Infections; Virus Cultivation2006-12-15
5 Digre, Kathleen B.; Gouw, Launce G.; Harris, Catherine P.; Haines, John H.; Ptacek, L.J.Autosomal dominant cerebellar ataxia with retinal degeneration: clinical, neuropathologic, and genetic analysis of a large kindred.The autosomal dominant cerebellar ataxias (ADCA) comprise a heterogeneous group of neurologic disorders characterized by degeneration of the cerebellum, spinal cord, and brainstem. Genetic analysis has revealed two loci, SCA1 on chromosome 6p, and SCA2 on chromosome 12q, responsible for some ADCA. W...Retinal Degeneration; Cerebellar Ataxia; Genetic Analysis1994-08
6 Digre, Kathleen B.; Blumenthal, Deborah T.; Cessna, Melissa H.; Gopez, Evelyn V.; Shen, YueCarcinomatous meningitis as the presenting manifestation of gallbladder carcinoma: case report and review of the literatureThe primary tumors that typically cause carcinomatous meningitis include lung cancer, breast cancer, leukemia, lymphoma and melanoma. A variety of neurological signs and symptoms can be seen depending on the extent and location of the meningeal metastasis. Once the diagnosis of carcinomatous meningi...Carcinomatous Meningitis; Gallbladder Carcinoma; Leptomeningeal Metastasis; Meningeal Carcinomatosis; Cranial Neuropathy2004-10
7 Brooking, Haisley; Bernard, Philip; Skliar, Mikhail; Zhang, HuananCharacterization of exosomesExosomes are nano-vesicles secreted by cells. They are found in extracellular space and all body fluids. This poster describes the methods for the isolation and characterization of the exosomes and my plans for future research.Cancer--Research2017
8 Owen, William E.; Roberts, William L.Comparable effects of DIGIBIND and DigiFab in thirteen digoxin immunoassaysDigoxin is widely prescribed for the treatment of cardiac conditions (1). Because of its narrow therapeutic range, digoxin-related toxicity resulting from acute or chronic overdose is common. Metabolites of digoxin as well as related compounds, including digitoxin, tanshinones, bufandienolide, a...DIGIBIND; DigiFab; Digoxin immunoassays; Digoxin toxicity2002
9 Jaskowski, Troy D.Comparison of capillary zone and immunosubtraction with agarose gel and immunofixation electrophoresis for detecting and identifying monoclonal gammopathiesCapillary zone electrophoresis (CZE) and immunosubtraction electrophoresis (ISE) were evaluated for ability to detect and immunotype monoclonal proteins, compared with agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE), respectively. Six hundred seventeen serum samples wer...Capillary zone electrophoresis; Immunosubtraction electrophoresis; CZE; ISE; Monoclonal protein electrophoresis; Serum protoin electrophoresis; Agarose gel; Dysproteinemias1999
10 Owen, William E.; Roberts, William L.Comparison of five automated serum and whole blood folate assaysSerum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonisotopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, wer...Folate assays; Serum folate; Whole blood folate; Automated assays2003
11 Jaskowski, Troy D.Comparison of four enzyme immunoassays with a western blot assay for the determination of type-specific antibodies to herpes simplex virusMost current enzyme immunoassays (EIAs) differentiate inadequately between types 1 and 2 herpes simplex virus (HSV) antibodies since significant crossreactivity exists. We compared 4 IgG type-specific EIAs using a Western blot assay for resolution of discrepant results. The Diamedix had sensitivit...Enzyme immunoassays; Western blot assay; Type-specific antibodies2001
12 Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R; Hillyard, David RComparison of the DIGENE HBV HYBRID CAPTURE II AND ROCHE COBAS Amplicor HBV Monitor Assays.Evaluate and compare the performance characteristics of the COBAS Amplicor HBV Monitor test (Roche Diagnostics Corp.) and the HBV Hybrid capture II (Digene Corporation) assay for the quantification of HBV BNA Levels.Quantitative Tests; Linearity Studies2001-08-19
13 Konnick, Eric Q.; Erali, Maria; Ashwood, Edward R.; Hillyard, David R.Comparison of the National Genetics Institute (NGI) HCV Superquant and Roche COBAS Amplicor HCV Monitor, Version 2.0 AssaysChronic Hepatitis C infection is currently recognized as an important health care problem. It is estimated that nearly 4 million Americans are infected, 15 to 20 percent of whom will eventually develop Cirrhosis. Current treatments for Hepatitis C virus (HCV) are limited to alfa-interferon alone or ...Cirrhosis; Patients2000-04-22
14 Kriesel, John D.; Baringer, J R; Spruance, Spotswood L.Correlation between detection of herpes simplex virus in oral secretions by PCR and susceptibility to experimental UV radiation-induced herpes labialis.We examined the oral secretions of 25 patients for herpes simplex virus (HSV) at the time of and following experimental UV radiation (UVR). HSV was detected in one or more oral secretion specimens in 5 of 12 (42%) cases by cell culture and in 8 of 12 (67%) cases by PCR. On the day of UVR, HSV was de...Polymerase Chain Reaction; Ultraviolet Rays; Simplexvirus1994
15 Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V.Cross platform comparison of melting curve analysisMelting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt...PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping2005-11-04
16 Owen, William E.; Roberts, William L.Cross-reactivity of three recombinant insulin analogs with five commercial insulin immunoassaysSeveral new insulin analogs that are prepared with recombinant DNA technology are available for clinical use [for a recent review, see Ref. (1)]. These agents have altered pharmacokinetics compared with regular human insulin. Insulin aspart (Novo-LogTM; Novo Nordisk Pharmaceuticals) is homologous ...Insulin analogs; Insulin immunoassays; Cross-reactivity2004
17 Hymas, Weston C.; Hillyard, David R.Description and validation of a novel real-time RT-PCR enterovirus assayEnteroviruses are a leading cause of aseptic meningitis in adult and pediatric populations. We describe the development of a real-time RT-PCR assay that amplifies a small target in the 5' nontranslated region upstream of the classical Rotbart enterovirus amplicon. The assay includes an RNA internal ...RT-PCR enterovirus assay; Rotbart enterovirus amplicon; Aseptic meningitis2008
18 Jaskowski, Troy D.Determination of cytokine responses using a multiplexed fluorescent microsphere immunoassayWe used a multiplexed fluorescent microsphere immunoassay to develop a sandwich capture assay to assess simultaneously the production of thymus helper (TH) 1- and TH2-type cytokines in tissue culture supernatant obtained from stimulated peripheral blood mononuclear cells. The assay then was use...Multiplexed fluorescent microsphere immunoassay2002
19 Owen, William E.; Rockwood, Alan L.; Roberts, William L.; Meikle, A. WayneDevelopment and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroidsCongenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17O...Congenital adrenal hyperplasia; Adrenal steroids; Tandem mass spectrometry assay2006
20 Owen, William E.; Roberts, William L.; Ring, Terry ArthurDevelopment and validation of an automated thawing and mixing workcellWorking toward a goal of total laboratory automation, we are automating manual activities in our highest volume laboratory section. Because half of all specimens arriving in this laboratory section are frozen, we began by developing an automated workcell for thawing frozen specimens and mixing the t...Automated workcell; Laboratory automation2007
21 Jaskowski, Troy D.Diagnostic performance of phospholipid-specific assays for the evaluation of antiphospholipid syndromeThe diagnostic performance of commercially available nonstandard antiphospholipid (aPL) assays for the evaluation of antiphospholipid syndrome (APS) is unknown. In 62 patients with APS, 88 with recurrent pregnancy loss, 50 healthy blood donors, and 24 women with one or more successful pregnancies, w...APS; Phospholipid-specific assays; Diagnostic performance2008-06-01
22 Cloud, Joann L.; Hoggan, Karen; Cohen, Samuel; Brown-Elliott, Barbara A.; Mann, Linda; Wilson, Rebecca; Aldous, Wade K.; Wallace, Jr, Richard J.; Woods, Gail L.Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probesThis poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is ...MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus2005-09-05
23 Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M.Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysisDifferentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar...MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR2006-01-09
24 Aldous, Jay A.; Engar, Richard C.Do dentists prescribe narcotics excessively?Dealing with pain is an inevitable sequela to dental treatment. Although several drug regimens primarily involving narcotics have been used in the past, availability of nonsteroidal anti-inflammatory drugs (NSAIDs) has increased recently. A study was conducted to analyze dental prescribing patterns ...Analgesics, Opioid; Anti-Inflammatory Agents; Dentist's Practice Patterns; Pain, Postoperative1996-07-01
25 Parker, Sam; Stevenson, Jeffery B.; Hillyard, David R.Eclipse probe assay for the detection of mycoplasma pneumoniae.Mycoplasma pneumoniae is an important human pathogen that causes community acquired pneumonias and upper respiratory tract infections. Traditional detection techniques, such as culture and serology, cannot rapidly aid in the diagnosis of an acute infection with adequate sensitivity and specificity. ...Eclipse Hybridization Probe; Protocol; Techniques2005-09-11
26 Normann, Richard A.; Chandler, John P.Effects of calcium ions on L-type horizontal cells in the isolated turtle retinaA technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p...Retina; Calcium; Horizontal Cell; Turtle1990
27 Owen, William E.; Roberts, William L.Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods.Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur...Glycohemoglobin; GHB; Glycated Hb; Glycemic control2008
28 Owen, William E.; Roberts, William L.Effects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assaysGlycated hemoglobin is widely used in the management of diabetes mellitus. At least 300,000 Americans with diabetes mellitus have the hemoglobin (Hb) C or S trait. The accuracy of HbA1c methods can be adversely affected by the presence of these traits. We evaluated the effects of HbC and HbS traits ...Glycated hemoglobin assays; Hemoglobin C trait; Hemoglobin S trait2008
29 Erickson, J. Alan; Aldeen, William E.; Ashwood, Edward R.Evaluation of a fecal pancreatic elastase-1 enzyme-linked immunosorbent assay: assessment versus an established assay and implication in classifying pancreatic functionDisagreement continues regarding the two commercially available fecal pancreatic elastase-1 (PE-1) ELISAs and their respective capabilities to assess pancreatic function. Our objectives were to validate the newer PE-1 ELISA and evaluate the test against the previously established assay, to investiga...PE-1; pancreatic elastase-1; FDA; AMR; analytical measurement range; BioServ Diagnostics PE-1ELISA; ScheBo Biotech PE-1ELISA2005-09-25
30 Jaskowski, Troy D.Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination controlToxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction...PCR probe capture assay; Uracil N-glycosylase; UNG2000
31 Erickson, J. Alan; Ashwood, Edward R.Evaluation of an enzyme-linked binding protein assay for hyaluronic acid and concentrations in hepatitis C infected patientsSerological hyaluronic acid (HA) has been proposed as a noninvasive alternative to liver biopsy for assessing the extent of liver fibrosis. Liver biopsy correctly identifies hepatic disease in about 65 to 75% of cases, being strongly dependent on the length of the biopsy obtained. Furthermore, hepat...Hyaluronic Acid Concentration; Corgenix HA assay2005-07-21
32 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the Alliance HCV Quantitative Analyte Specific Reagent (ASR) Assay and Comparison to the COBAS HCV TaqMan ASR Assay.To evaluate the Abbott/Celera Alliance HCV Quantitative ASR (Alliance) assay using the Qiagen BioRobot 9604 and QiaAmp Virus Kit for viral RNA isolation. And compare Alliance HCV TaqMan ASR to the Roche COBAS HCV TaqMan ASR (CTM) assay calibrated using Armored RNA solutions and WHO 2nd international...Quantitative Tests; Linearity Studies2004-05-10
33 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) and COBAS HCV TaqMan ASR Assays using the Vidiera NSP sample preparation instrument.The Vidiera Nsp nucleic sample preparation instrument (Vidiera,Beckman-Coulter Inc., Fullerton CA) was evaluated using the COBAS HBV TaqMan Analyte Specific Reagent (ASR. Roche Molecular Diagnostics [RMD], Indianapolis, IN) and COBAS HCV TaqMan ASR (RMD) assays using clinical samples and HCV Armored...Quantitative Tests; Linearity Studies2006-05-05
34 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.Evaluation of the COBAS HBV TaqMan Analyte Specific Regent (ASR) Assay and Comparison to the COBAS Amplicor HBV Monitor Assay.erformance characteristics of the COBAS HBV TaqMan ASR (TaqMan) assay were evaluated and compared to the COBAS Amplicor HBV Monitor test (Amplicor) assay for the quantification of HBV DNA in clinical samples.Quantitative Tests; Linearity Studies2004-05-03
35 Konnick, Eric Q.; Ashwood, Edward R.; Hillyard, David R.; Comanor, Lorraine; Di Canzio, JamesEvaluation of the Versant ? HBV DNA 3.0 Assay (bDNA)+ and comparison with the Cobastm HBV Taqman Analyte Specific Reagent Assay.To evaluate the performance characteristics of the Versant ? HBV DNA 3.0 assay (bDNA)+ (Versant, Bayer Healthcare LLC, Tarrytown, NY). 2.) To compare the Versant assay to the COBAS HBV TaqMan Analyte Specific Reagent assay (TaqMan) (Roche Molecular Systems, Pleasanton, CA) using random and defined p...International Standard; HBV DNA Tests2005-05-07
36 Wittwer, Carl T.Genotyping accuracy of high-resolution DNA melting instrumentsHigh-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assesse...2014-01-01
37 Liew, Michael; Erali, Maria; Page, Sam; Hillyard, David R.; Wittwer, Carl T.Hepatitis C genotyping by denaturing high-performance liquid chromatographyDetermination of the hepatitis C virus (HCV) genotype for infected patients increasingly has become accepted as the standard of care. Genotype assignment helps in assessing disease prognosis and assists in establishing the appropriate duration of treatment. The great genetic diversity of HCV, with 1...Base Sequence; 5' Untranslated Regions; Genotype; Hepacivirus; Molecular Sequence Data2004-01
38 Dwight, Zachary LawrenceHeterozygote PCR product melting curve predictionMelting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four...2014-01-01
39 Erickson, J. Alan; Smith, Jeffrey, J.; Bornhorst, Joshua A.; Ashwood, Edward R.Humananti-mouse antibody protected ELISA for the quantification of squamous cell carcinoma antigenSquamous cell carcinoma antigen (SCC) was first isolated from squamous cell carcinoma tissue of the uterine cervix and initially reported as TA-4. The antigen has been characterized as a glycoprotein with a molecular weight between 45,000 and 55,000 daltons. Studies suggest that SCC may function as ...SCC; Squamous Cell Carcinoma Antigen; BSA; PBS; Phosphate Buffered Saline; HAMA; Human Anti-mouse Antibodies; IIR; ImmunoglobulinInhibiting Reagent2005-12-09
40 Pounder, June I.; Simmon, Keith E.; Barton, Claudia A.; Hohmann, Sheri L.Identification of nonsporulating molds by sequencing internal transcribed spacer regions with Virodec software and databaseFungal infections are increasing, particularly among immunocompromised hosts, and a rapid, accurate diagnosis is essential for the initiation of targeted antifungal therapy. Identification of fungi from culture requires the presence of reproductive structures, and the absence of spores can increase ...nonsporulating molds; genetic sequencing; Virodec; ITS I; ITS II2006-01-09
41 Wittwer, Carl T.Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instrumentsBACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS: A continuous assay that monitors DNA polymerase extension us...2014-01-01
42 Cloud, Joann L.; Carroll, Karen C.; Cohen, Samuel; Anderson, Clint M.; Woods, Gail L.Interpretive criteria for use of AccuProbe for identification ofRapid identification of Mycobacterium avium complex (MAC) is possible by use of AccuProbe (Gen-Probe, San Diego, Calif.). To evaluate the reliability of the MAC AccuProbe for testing 7H9 cultures inoculated with broth from MGIT cultures positive for acid-fast bacilli or growth on a s...Bacteria; Typing; Mycobacterium avium complex; MAC2005-03-03
43 Planelles, VicenteKinase control of latent HIV-1 infection: PIM-1 kinase as a major contributor to HIV-1 reactivationDespite the clinical relevance of latent HIV-1 infection as a block to HIV-1 eradication, the molecular biology of HIV-1 latency remains incompletely understood. We recently demonstrated the presence of a gatekeeper kinase function that controls latent HIV-1 infection. Using kinase array analysis w...2014-01-01
44 Jaskowski, Troy D.; Litwin, Christine M.; Hill, Harry R.Laboratory diagnosis of Inflammatory Bowel Disease (IBD)Study to compare the results obtained by two separate reference libraries for serological assays utlilized in the diagnosis and differentiation of Crohn's disease and ulcerative colitis, and to assess the clinical utility of the outer-membrane porin C (OmpC) IgA assay in IBD.IBD2005-06-17
45 Dwight, Zachary LawrenceLaboratory driven, lean-to-adaptive prototyping in Parallel for Web software project identification and application development in health science researchClinical research laboratories, bioinformatics core facilities, and health science organizations often rely on heavy planning based software development models to propose, build, and distribute software as a consumable product. Projects in non-agile software life cycles tend to have rigid ?plan-desi...2012
46 Smith, A. Gordon; Singleton, J. RobinsonLifestyle intervention for pre-diabetic neuropathy.OBJECTIVE: The purpose of this study was to evaluate intraepidermal nerve fiber density (IENFD) as a sensitive measure of neuropathy change in patients with neuropathy associated with impaired glucose tolerance (IGT) receiving lifestyle intervention based on that used in the Diabetes Prevention Prog...Older people; Biopsy; Blood Pressure; Body Mass Index; Cholesterol; Diabetic Diet2006-06-29
47 Margraf, Rebecca L.; Mao, Rong; Highsmith, W. Edward; Holtegaard, Leonard; Wittwer, Carl T.Masking selected sequence variation by incorporating mismatches into melting analysis probesHybridization probe melting analysis can be complicated by the presence of sequence variations (benign polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Selected sequence variati...probe hybridization; high-resolution melting analysis; masking; RET2005-06-28
48 Margraf, Rebecca L.; Mao, Rong; Wittwer, Carl T.Masking techniques: masking selected sequence variation by incorporating mismatches into melting analysis probesHybridization probe melting analysis can be complicated by the presence of sequence variation (non-pathogenic polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Materials and Meth...probe hybridization; high-resolution melting analysis; masking; RET2005-10-21
49 Rockwood, Alan L.Mass spectrometry for steroids, immunosuppressants, and other clinical applicationsPresentation slides on matching mass spectrometry technology to clinical applications.Immunosuppressants2005-07-26
50 Singleton, J. Robinson; Smith, A. GordonMicrovascular complications of impaired glucose tolerance.Impaired glucose tolerance (IGT) serves as a marker for the state of insulin resistance and predicts both large- and small-vessel vascular complications, independent of a patient's progression to diabetes. Patients with IGT are at significantly increased risk for death and morbidity due to myocardia...Microcirculation; Humans2003-12-01
51 Eichwald, Ernst; Capecchi, Mario R.; Thomas, Kirk R.Mouse model for the delta F508 allele of cystic fibrosisThe most common cause of cystic fibrosis is a mutation that deletes phenylalanine 508 in cystic fibrosis transmembrane conductance regulator (CFTR). The delta F508 protein is misprocessed and degraded rather than traveling to the apical membrane. We used a novel strategy to introduce the delta F508 ...Digestive System; Disease Models, Animal; Electrolytes; Mice, Inbred C57BL1995-10
52 Jaskowski, Troy D.Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay resultsWe hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measur...Heterophil antibodies; Heterophil interference; Multiplex analysis; False-positive HIV enzyme-linked immunosorbent assay; ELISA2001
53 Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T.Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysisThis poster describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the method...Genotyping; Amplicon Melting2005-06
54 Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T.Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis [abstract]This abstract describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the meth...Genotyping; Amplicon Melting2005-06-22
55 Cloud, Joann L.; Meyer, Jay J.; Pounder, June I.; Woods, Gail L.Mycobacterium arupense sp. nov., a novel moderately growing nonchromogenic bacterium isolated from clinical specimensThis author manuscript discusses how several isolates of Mycobacterium species related to the M. terrae complex have been isolated from clinical samples. In the clinical microbiology laboratory, partial 16S rRNA gene sequencing (approximate first 500 base pairs) is often used to identify Mycobacteri...Mycobacterium arupense; Mycobacterium nonchromogenicum; Mycobacterium terrae; Mycobacterium triviale; MCRO 62005-11-22
56 Hymas, Weston C.; Hillyard, David R.Novel one step real time RT-PCR Assay for the detection of Enterovirusnteroviruses (EV) are the leading cause of aseptic meningitis in pediatric and adult populations and can be associated with severe disease such as myocarditis, encephalitis, and paralytic poliomyelitis. RT-PCR has rapidly become the diagnostic methodology of choice due to its sensitivity and rapid t...Viruses; Isolation; Purification2005-07-11
57 Hymas, Weston C.; Hillyard, David R.Novel one step real time RT-PCR assay for the detection of Enterovirus, AThis poster describes the development of a novel real time assay that utilizes primers and an Eclipse probe in an overlapping format upstream of the Rotbart amplicon.RT-PCR Assay2005-11-08
58 Kriesel, John D.; Spruance, Spotswood L.; Daynes, Raymond A.Nucleic acid vaccine encoding gD2 protects mice from herpes simplex virus type 2 disease.Nucleic acid vaccinations with plasmids pWW65, containing the sequence for herpes simplex type 2 (HSV-2) gD2, and pRSVnt, lacking the gD sequence, were studied. Groups of mice were immunized with pWW65 alone, pWW65 plus 1,25-dihydroxyvitamin-D3 (D3), or pRSVnt. Clinical disease (vaginitis), serum an...Mice, Inbred BALB C; Plasmids; Viral Vaccines1996
59 Hansen, Mark S.; Coffin, Cheryl M.; Capecchi, Mario R.Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function: implications for alveolar rhabdomyosarcoma cell of origin.To investigate the role of the translocation-associated gene Pax3:Fkhr in alveolar rhabdomyosarcomas, we generated a Cre-mediated conditional knock-in of Pax3:Fkhr into the mouse Pax3 locus. Exploring embryonic tumor cell origins, we replaced a Pax3 allele with Pax3:Fkhr throughout its expression do...Neuroprogenitor; Embryogenesis2004-11-01
60 Owen, William E.; Roberts, William L.Performance characteristics of an HPLC method for Urinary AlbuminMicroalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detecti...MA; Microalbuminuria; HPLC2005-07-06
61 Owen, William E.; Roberts, William L.Performance characteristics of eight estradiol immunoassaysMeasurement of estradiol is useful in assisted reproduction, evaluation of infertility, menopause, and male feminization. The analytic performance of 8 estradiol immunoassays was evaluated. The imprecision and accuracy of the Access, ADVIA Centaur, ARCHITECT i2000, AutoDELFIA, Elecsys 2010, IMMULI...Estradiol immunoassays2004
62 Owen, William E.; Roberts, William L.Performance characteristics of four automated natriuretic peptide assaysMeasurement of circulating B-type natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP) can identify patients with heart failure and guide therapy. The limit of detection, linearity, imprecision, method comparison, analytic concordance, and reference intervals of the Access 2 BNP (Biosite, San...Natriuretic peptide assays; BNP2005
63 Owen, William E.; Roberts, William L.Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluidMeasurement of IgA and IgM in cerebrospinal fluid (CSF) can be useful in the diagnosis of multiple sclerosis and other central nervous system disorders. The Dade Behring (Deerfield, IL) N Latex IgA and N Latex IgM tests on the BN II System and Beckman Coulter (Brea, CA) low-concentration IgA an...IgA; IgM; Immunonephelometric assays; Imprecision; Method comparison; Reference interval2003
64 Slev, Patricia R.; Rawlins, Mindy, L.; Roberts, William L.Performance characteristics of seven automated CA 15-3 methodsPoster describing studies to evaluate seven automated methods for the following parameters: limit of detection, linearity, imprecision, reference intervals and method comparison with the ADVIA Centaur as the comparison method to detect CA 15-3.CA 15-3 Method2005-07-13
65 Mongia, Shella K.; Rawlins, Mindy, L.; Owen, William E.; Roberts, William L.Performance characteristics of seven automated CA125 assaysAuthor manuscript. Cancer antigen 125 (CA125) is a high molecular mass glycoprotien, which is used as tumor marker to monitor disease progression, response to therapy and in early detection of recurrence after treatment for ovarian cancer. The Access 2, ADVIA Centaur, ARCHITECT i2000, AxSYM, Elecsys...CA125 assays2005-08-29
66 Owen, William E.; Martins, Thomas B.; Litwin, Christine M.; Roberts, William L.Performance characteristics of six IMMULITE 2000 TORCH assaysTORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (like syphilis), rubella (RV), cytomegalovirus (CMV), and herpesviruses (HSV) that are associated with congenital abnormalities during maternal infection. Prenatal testing for antibodies against these agents is one tool for preve...TORCH; Toxoplasma gondii; syphilis virus; rubella; cytomegalovirus; herpesvirus; IMMULITE 2000; congenital abnormalities; laboratory testing2006-01-04
67 Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R; Hillyard, David RPerformance characteristics of the COBAS HCV TaqMan ASR and Comparison to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0.Evaluate the COBAS HCV TaqMan ASR assay using both the Qiagen BioRobot 9604 and a 1mL extraction protocol for HCV RNA isolation. And Compare the COBAS TaqMan HCV ASR assay to the COBAS Amplicor HCV Monitor, Version 2.0 and Versant HCV bDNA 3.0 assays.Quantitative Tests; Linearity Studies2002-11-21
68 Konnick, Eric Q.; Williams, Sheri M.; Ashwood, Edward R.; Hillyard, David R.Performance Characteristics of the COBAS HCV TaqMan ASR Using Armored RNA CalibratorsAs improved therapies have become available for Hepatititis C Virus (HCV) infection, the use of assays to quantitate HCV RNA has increased dramatically (3, 14, 16). The initial use of these assays was to predict the likelihood of therapy based on baseline HCV RNA levels. These reports indicated that...Roche Amplicor Monitor; NGI Superquant; patients2003-04-22
69 Rockwood, Alan L.Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometryBackground: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show...2014-01-01
70 Jaskowski, Troy D.Polymerase chain reaction detection of Lyme disease: correlation with clinical manifestations and serologic responsesThe Author's have developed a simple, nested polymerase chain reaction (PCR) assay for amplification of an outer surface protein A (OspA) gene fragment of Borrelia burgdorferi using rapid temperature cycling and ethidium bromide detection on agarose gels, and applied it to the diagnosis of Lyme dis...PCR; Rapid cycle amplification1996
71 Hillyard, David R.Quantitative real time PCR assay for detecting BK virus in serum, plasma and urine.BK is a non-enveloped virus in the Polyomavirus family, closely related to SV40 and JC virus. Primary infection with BK generally occurs during childhood without specific symptoms, and is widespread in the population, with approximately 80% of adults infected globally. The virus remains latent in th...Quantitative Tests; Linearity Studies; Oligonucleotides2005-05-02
72 Stevenson, Jeffery B.; Hillyard, David R.Quantitative real time PCR assay for detecting EBV virus in multiple sample types.We are developing a real time quantitative PCR assay to detect EBV in serum, plasma, whole blood, tissue and spinal fluid. Real time PCR, with its intrinsic quantitative capacity, is an excellent method for measuring EBV viral load. Epstein Barr virus is a member of the Herpesvirus family, with a tr...Eclipse Probes; EBV Primer2005-08-11
73 Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Bill; Hillyard, David R.Quantitative real time PCR assay for the detection of human herpes 6.Human herpes virus type 6 is a beta virus that occurs as two variants (A & B) and infects nearly 100% of the population before two years of age. Primary infections in children can produce fever and a unique lacy rash that gives the disease its name: roseola. The rare primary infection in adults can ...Probe Technology; Roseola Infantum2005-02-05
74 Hymas, Weston C.; Hillyard, David R.Real time PCR assay for the detection of CMV using eclipse hybridization probesWe developed a qualitative real time PCR assay to detect CMV in patient samples, using a novel hybridization probe termed the Eclipse probe (Epoch Biosciences). These are single probes with a fluorescent molecule attached to the 3' end and a dark quencher on the 5' end. Also conjugated to the 5' end...Tissues Analysis; Elegans2004-08-11
75 Stevenson, Jeff; Procter, Melinda; Hillyard, David R.Real time PCR assays for the detection of viral pathogens - are they the gold standard?Over the past decade, PCR testing for infectious agents in the clinical laboratory has begun to replace other methods, such as culture. PCR is often now referred to as the 'gold standard' in this field. Further evolution of this technology has seen the introduction of real time PCR, replacing tradit...Polymorphism; Target DNA2003-11-11
76 Hymas, Weston C.; Hillyard, David R.Real time RT-PCR assay for norovirus detection with eclipse probes and a non-competitive internal control.Noroviruses are the major causative agents of nonbacterial gastroenteritis worldwide and are estimated to cause approximately 23 million cases in the United States annually. Real time RT-PCR is rapidly becoming the principle diagnostic means for norovirus detection due to its enhanced sensitivity an...Gastroenteritis; Eclipse Hybridization Probes; Norovirus2005-05-03
77 Petti, Cathy A.Role of the laboratory in diagnosis of influenza during seasonal epidemics and potential pandemicsLaboratory diagnosis of influenza is critical to its treatment and surveillance. With the emergence of novel and highly pathogenic avian influenza viruses, the role of the laboratory has been further extended to include isolation and subtyping of the virus to monitor its appearance and facilitate ap...Antibodies, Viral; Antigens, Viral; RNA, Viral2006
78 Jaskowski, Troy D.Screening for antinuclear antibodies by enzyme immunoassayIndirect fluorescent antibody (IFA) is the most widely used method in clinical laboratories to screen for autoantibodies against a wide variety of nuclear antigens. Recently, a number of antinuclear antibody (ANA) enzyme immunoassay (EIA) screens have become commercially available and claim to be an...Antinuclear antibodies; Enzyme immunoassay1996
79 Cloud, Joann L.; Dunn, Jim; Hoggan, Karen; Wilson, Deborah; Iwen, Peter; Lasala, Paul; Hall, Geri; Woods, Gail L.Sequence-based identification of gram negative nonfermenters using commercial 16S rDNA databasesThis poster presents a study assessing the validity of the databases from two commercial systems were compared for the identification of NF. Background: Identification of gram negative nonfermenters (NF) from cultured human specimens is often difficult using traditional phenotypic methods. Partial 1...MicroSeq500; Virodec2005-08-29
80 Aldous, Wade K.; Hymas, Weston C.; Stevenson, Jeffery B.; Taggart, Edward W.;Hillyard, David R.Single tube real-time RT-PCR Assay for the detection of enterovirusWe developed a rapid and sensitive method for the routine detection of enterovirus in CSF and plasma using a one tube, one step, real-time PCR assay. A primer set and an Eclipse probe were designed to target the highly conserved 5' untranslated region of the enterovirus genome. This assay was compar...MGB Eclipse; Viral RNA; Picornaviridae2004-09-11
81 Roberts, William L.Specificity characteristics of 7 commercial creatinine measurement procedures by enzymatic and jaffe method principlesStandardized calibration does not change a creatinine measurement procedure?s susceptibility to potentially interfering substances.2012
82 Dwight, Zachary LawrenceuAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictionsAbstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce...2012-01-01
83 Hymas, Weston C.Ultrasonic enhancement of antibiotic action on several species of bacteriaThe effect of the antibiotics gentamicin, streptomycin, kanamycin, tetracycline, and ampicillin on planktonic cultures of Enterobacter aerogenes, Serratia marcescens, Salmonella derby, Streptococcus mitis, and Staphylococcus epidermidis with and without an application of 70 kHz ultrasound was studi...Enterobacter aerogenes; Serratia marcescens; Salmonella derby; Streptococcus mitis; Staphylococcus epidermidis; Planktonic cultures; Gentamicin; Streptomycin; Kanamycin; Tetracycline; Ampicillin; Ultrasonic enhancement; Bioacoustic effect1998
84 Dwight, Zachary LawrenceuMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web applicationuMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono...2011
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