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Creator | Title | Description | Subject | Date |
1 |
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Seipp, Michael; Williams, Jamie; Meadows, Cindy; Wittwer, Carl T. | Multiplex solution genotyping without probes of the factor V Leiden (G1691A), Prothrombin (G20210A), and MTHFR (C677T and A1298C) mutations in one tube by single color high-resolution melting analysis [abstract] | This abstract describes a study which concludes that genotyping by amplicon melting is a rapid, closed-tube method for genotyping without probes that can be multiplexed. The method was successfully applied to the four most common clotting factor mutations in a single tube. The simplicity of the meth... | Genotyping; Amplicon Melting | 2005-06-22 |
2 |
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Jaskowski, Troy D.; Litwin, Christine M.; Hill, Harry R. | Laboratory diagnosis of Inflammatory Bowel Disease (IBD) | Study to compare the results obtained by two separate reference libraries for serological assays utlilized in the diagnosis and differentiation of Crohn's disease and ulcerative colitis, and to assess the clinical utility of the outer-membrane porin C (OmpC) IgA assay in IBD. | IBD | 2005-06-17 |
3 |
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Pounder, June I.; Simmon, Keith E.; Barton, Claudia A.; Hohmann, Sheri L. | Identification of nonsporulating molds by sequencing internal transcribed spacer regions with Virodec software and database | Fungal infections are increasing, particularly among immunocompromised hosts, and a rapid, accurate diagnosis is essential for the initiation of targeted antifungal therapy. Identification of fungi from culture requires the presence of reproductive structures, and the absence of spores can increase ... | nonsporulating molds; genetic sequencing; Virodec; ITS I; ITS II | 2006-01-09 |
4 |
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Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M. | Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysis | Differentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar... | MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR | 2006-01-09 |
5 |
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Owen, William E.; Roberts, William L.; Ring, Terry Arthur | Development and validation of an automated thawing and mixing workcell | Working toward a goal of total laboratory automation, we are automating manual activities in our highest volume laboratory section. Because half of all specimens arriving in this laboratory section are frozen, we began by developing an automated workcell for thawing frozen specimens and mixing the t... | Automated workcell; Laboratory automation | 2007 |
6 |
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Cloud, Joann L.; Hoggan, Karen; Cohen, Samuel; Brown-Elliott, Barbara A.; Mann, Linda; Wilson, Rebecca; Aldous, Wade K.; Wallace, Jr, Richard J.; Woods, Gail L. | Differentiation of mycobacterium chelonae and M. abscessus using SmartCycler PCR and MGB eclipse probes | This poster discusses how the use of partial 16S rDNA sequencing (first one-third of the gene) for the identification of Mycobacterium species from cultured isolates has become recognized as a very accurate method for identification. The distinction between M. chelonae and M. abscessus, however, is ... | MGB Eclipse System; SmartCycler PCR; Mycobacterium abscessus | 2005-09-05 |
7 |
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Owen, William E.; Roberts, William L. | Effects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assays | Glycated hemoglobin is widely used in the management of diabetes mellitus. At least 300,000 Americans with diabetes mellitus have the hemoglobin (Hb) C or S trait. The accuracy of HbA1c methods can be adversely affected by the presence of these traits. We evaluated the effects of HbC and HbS traits ... | Glycated hemoglobin assays; Hemoglobin C trait; Hemoglobin S trait | 2008 |
8 |
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Dwight, Zachary Lawrence | uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application | uMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest-neighbor stacking energies, loop entropy effects, cation (mono... | | 2011 |
9 |
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Owen, William E.; Roberts, William L. | Cross-reactivity of three recombinant insulin analogs with five commercial insulin immunoassays | Several new insulin analogs that are prepared with recombinant DNA technology are available for clinical use [for a recent review, see Ref. (1)]. These agents have altered pharmacokinetics compared with regular human insulin. Insulin aspart (Novo-LogTM; Novo Nordisk Pharmaceuticals) is homologous ... | Insulin analogs; Insulin immunoassays; Cross-reactivity | 2004 |
10 |
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Vaughn, Cecily P.; Crockett, David K.; Lim, Megan S.; Elenitoba-Johnson, Kojo S.J. | Analytical characteristics of ICAT-LC-MS/MS for quantitative proteomics studies | Recent studies have employed isotope-coded affinity tags (ICAT) in concert with tandem mass spectrometry (MS/MS) to assess differential expression of proteins on a proteomic scale and perform unbiased quantitative comparisons between samples from different physiological or disease states. We sought ... | isotope-coded affinity tags; ICAT; mass spectrometry; MS/MS; proteomic scale; FL | 2005-06-03 |
11 |
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Owen, William E.; Roberts, William L. | Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluid | Measurement of IgA and IgM in cerebrospinal fluid (CSF) can be useful in the diagnosis of multiple sclerosis and other central nervous system disorders. The Dade Behring (Deerfield, IL) N Latex IgA and N Latex IgM tests on the BN II System and Beckman Coulter (Brea, CA) low-concentration IgA an... | IgA; IgM; Immunonephelometric assays; Imprecision; Method comparison; Reference interval | 2003 |
12 |
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Owen, William E.; Roberts, William L. | Comparable effects of DIGIBIND and DigiFab in thirteen digoxin immunoassays | Digoxin is widely prescribed for the treatment of cardiac conditions (1). Because of its narrow therapeutic range, digoxin-related toxicity resulting from acute or chronic overdose is common. Metabolites of digoxin as well as related compounds, including digitoxin, tanshinones, bufandienolide, a... | DIGIBIND; DigiFab; Digoxin immunoassays; Digoxin toxicity | 2002 |
13 |
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Dwight, Zachary Lawrence | Heterozygote PCR product melting curve prediction | Melting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four... | | 2014-01-01 |
14 |
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Owen, William E.; Roberts, William L. | Comparison of five automated serum and whole blood folate assays | Serum and whole blood folate measurements are used to establish folate deficiency. Most methods used in clinical laboratories are automated, nonisotopic methods that use folate-binding protein. Linearity, imprecision, and method comparison studies, including serum and whole blood hemolysates, wer... | Folate assays; Serum folate; Whole blood folate; Automated assays | 2003 |
15 |
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Jaskowski, Troy D. | Diagnostic performance of phospholipid-specific assays for the evaluation of antiphospholipid syndrome | The diagnostic performance of commercially available nonstandard antiphospholipid (aPL) assays for the evaluation of antiphospholipid syndrome (APS) is unknown. In 62 patients with APS, 88 with recurrent pregnancy loss, 50 healthy blood donors, and 24 women with one or more successful pregnancies, w... | APS; Phospholipid-specific assays; Diagnostic performance | 2008-06-01 |
16 |
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Petti, Cathy A.; Polage, Christopher R. | Assessment of the utility of viral culture of cerebrospinal fluid | Nucleic acid amplification testing is the preferred method to detect enteroviruses and Herpesviridae in cerebrospinal fluid, but clinicians still request viral culture. Review of 22,394 viral cultures of cerebrospinal fluid samples found that <0.1% recovered nonenterovirus, non-Herpesviridae species... | Enterovirus Infections; Herpesviridae Infections; Virus Cultivation | 2006-12-15 |
17 |
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Slev, Patricia R.; Rawlins, Mindy, L.; Roberts, William L. | Performance characteristics of seven automated CA 15-3 methods | Poster describing studies to evaluate seven automated methods for the following parameters: limit of detection, linearity, imprecision, reference intervals and method comparison with the ADVIA Centaur as the comparison method to detect CA 15-3. | CA 15-3 Method | 2005-07-13 |
18 |
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Jaskowski, Troy D. | Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay results | We hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measur... | Heterophil antibodies; Heterophil interference; Multiplex analysis; False-positive HIV enzyme-linked immunosorbent assay; ELISA | 2001 |
19 |
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Owen, William E.; Roberts, William L. | Performance characteristics of an HPLC method for Urinary Albumin | Microalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detecti... | MA; Microalbuminuria; HPLC | 2005-07-06 |
20 |
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Owen, William E.; Roberts, William L. | Performance characteristics of eight estradiol immunoassays | Measurement of estradiol is useful in assisted reproduction, evaluation of infertility, menopause, and male feminization. The analytic performance of 8 estradiol immunoassays was evaluated. The imprecision and accuracy of the Access, ADVIA Centaur, ARCHITECT i2000, AutoDELFIA, Elecsys 2010, IMMULI... | Estradiol immunoassays | 2004 |
21 |
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Kriesel, John D.; Baringer, J R; Spruance, Spotswood L. | Correlation between detection of herpes simplex virus in oral secretions by PCR and susceptibility to experimental UV radiation-induced herpes labialis. | We examined the oral secretions of 25 patients for herpes simplex virus (HSV) at the time of and following experimental UV radiation (UVR). HSV was detected in one or more oral secretion specimens in 5 of 12 (42%) cases by cell culture and in 8 of 12 (67%) cases by PCR. On the day of UVR, HSV was de... | Polymerase Chain Reaction; Ultraviolet Rays; Simplexvirus | 1994 |
22 |
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Mongia, Shella K.; Rawlins, Mindy, L.; Owen, William E.; Roberts, William L. | Performance characteristics of seven automated CA125 assays | Author manuscript. Cancer antigen 125 (CA125) is a high molecular mass glycoprotien, which is used as tumor marker to monitor disease progression, response to therapy and in early detection of recurrence after treatment for ovarian cancer. The Access 2, ADVIA Centaur, ARCHITECT i2000, AxSYM, Elecsys... | CA125 assays | 2005-08-29 |
23 |
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Margraf, Rebecca L.; Mao, Rong; Highsmith, W. Edward; Holtegaard, Leonard; Wittwer, Carl T. | Masking selected sequence variation by incorporating mismatches into melting analysis probes | Hybridization probe melting analysis can be complicated by the presence of sequence variations (benign polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Selected sequence variati... | probe hybridization; high-resolution melting analysis; masking; RET | 2005-06-28 |
24 |
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Owen, William E.; Roberts, William L. | Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods. | Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur... | Glycohemoglobin; GHB; Glycated Hb; Glycemic control | 2008 |
25 |
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Owen, William E.; Roberts, William L. | Performance characteristics of four automated natriuretic peptide assays | Measurement of circulating B-type natriuretic peptide (BNP) and N-terminal proBNP (NT-proBNP) can identify patients with heart failure and guide therapy. The limit of detection, linearity, imprecision, method comparison, analytic concordance, and reference intervals of the Access 2 BNP (Biosite, San... | Natriuretic peptide assays; BNP | 2005 |