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| Creator | Title | Description | Subject | Date | ||
|---|---|---|---|---|---|---|
| 1 |
 | Wittwer, Carl T. | Genotyping accuracy of high-resolution DNA melting instruments | High-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assesse... | 2014-01-01 | |
| 2 |
 | Dwight, Zachary Lawrence | Heterozygote PCR product melting curve prediction | Melting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four... | 2014-01-01 | |
| 3 |
 | Wittwer, Carl T. | Influence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments | BACKGROUND: Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS: A continuous assay that monitors DNA polymerase extension us... | 2014-01-01 | |
| 4 |
 | Planelles, Vicente | Kinase control of latent HIV-1 infection: PIM-1 kinase as a major contributor to HIV-1 reactivation | Despite the clinical relevance of latent HIV-1 infection as a block to HIV-1 eradication, the molecular biology of HIV-1 latency remains incompletely understood. We recently demonstrated the presence of a gatekeeper kinase function that controls latent HIV-1 infection. Using kinase array analysis w... | 2014-01-01 | |
| 5 |
 | Rockwood, Alan L. | Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometry | Background: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show... | 2014-01-01 |
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