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Filters: Department: "Pathology" Collection: "ir_uspace"
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CreatorTitleDescriptionSubjectDate
1 Liew, Michael; Erali, Maria; Page, Sam; Hillyard, David R.; Wittwer, Carl T.Hepatitis C genotyping by denaturing high-performance liquid chromatographyDetermination of the hepatitis C virus (HCV) genotype for infected patients increasingly has become accepted as the standard of care. Genotype assignment helps in assessing disease prognosis and assists in establishing the appropriate duration of treatment. The great genetic diversity of HCV, with 1...Base Sequence; 5' Untranslated Regions; Genotype; Hepacivirus; Molecular Sequence Data2004-01
2 Stevenson, Jeff; Procter, Melinda; Hillyard, David R.Real time PCR assays for the detection of viral pathogens - are they the gold standard?Over the past decade, PCR testing for infectious agents in the clinical laboratory has begun to replace other methods, such as culture. PCR is often now referred to as the 'gold standard' in this field. Further evolution of this technology has seen the introduction of real time PCR, replacing tradit...Polymorphism; Target DNA2003-11-11
3 Owen, William E.; Roberts, William L.Performance characteristics of four immunonephelometric assays for the quantitative determination of IgA and IgM in cerebrospinal fluidMeasurement of IgA and IgM in cerebrospinal fluid (CSF) can be useful in the diagnosis of multiple sclerosis and other central nervous system disorders. The Dade Behring (Deerfield, IL) N Latex IgA and N Latex IgM tests on the BN II System and Beckman Coulter (Brea, CA) low-concentration IgA an...IgA; IgM; Immunonephelometric assays; Imprecision; Method comparison; Reference interval2003
4 Margraf, Rebecca L.; Mao, Rong; Wittwer, Carl T.Masking techniques: masking selected sequence variation by incorporating mismatches into melting analysis probesHybridization probe melting analysis can be complicated by the presence of sequence variation (non-pathogenic polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Materials and Meth...probe hybridization; high-resolution melting analysis; masking; RET2005-10-21
5 Owen, William E.; Roberts, William L.Comparable effects of DIGIBIND and DigiFab in thirteen digoxin immunoassaysDigoxin is widely prescribed for the treatment of cardiac conditions (1). Because of its narrow therapeutic range, digoxin-related toxicity resulting from acute or chronic overdose is common. Metabolites of digoxin as well as related compounds, including digitoxin, tanshinones, bufandienolide, a...DIGIBIND; DigiFab; Digoxin immunoassays; Digoxin toxicity2002
6 Owen, William E.; Roberts, William L.Performance characteristics of an HPLC method for Urinary AlbuminMicroalbuminuria is a marker of early diabetic nephropathy and cardiovascular risk. Immunoassays for urinary albumin can underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that employs size exclusion HPLC with UV detecti...MA; Microalbuminuria; HPLC2005-07-06
7 Jaskowski, Troy D.Comparison of capillary zone and immunosubtraction with agarose gel and immunofixation electrophoresis for detecting and identifying monoclonal gammopathiesCapillary zone electrophoresis (CZE) and immunosubtraction electrophoresis (ISE) were evaluated for ability to detect and immunotype monoclonal proteins, compared with agarose gel electrophoresis (AGE) and immunofixation electrophoresis (IFE), respectively. Six hundred seventeen serum samples wer...Capillary zone electrophoresis; Immunosubtraction electrophoresis; CZE; ISE; Monoclonal protein electrophoresis; Serum protoin electrophoresis; Agarose gel; Dysproteinemias1999
8 Dwight, Zachary LawrenceuAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictionsAbstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce...2012-01-01
9 Hansen, Mark S.; Coffin, Cheryl M.; Capecchi, Mario R.Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function: implications for alveolar rhabdomyosarcoma cell of origin.To investigate the role of the translocation-associated gene Pax3:Fkhr in alveolar rhabdomyosarcomas, we generated a Cre-mediated conditional knock-in of Pax3:Fkhr into the mouse Pax3 locus. Exploring embryonic tumor cell origins, we replaced a Pax3 allele with Pax3:Fkhr throughout its expression do...Neuroprogenitor; Embryogenesis2004-11-01
10 Rockwood, Alan L.Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometryBackground: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show...2014-01-01
11 Petti, Cathy A.Role of the laboratory in diagnosis of influenza during seasonal epidemics and potential pandemicsLaboratory diagnosis of influenza is critical to its treatment and surveillance. With the emergence of novel and highly pathogenic avian influenza viruses, the role of the laboratory has been further extended to include isolation and subtyping of the virus to monitor its appearance and facilitate ap...Antibodies, Viral; Antigens, Viral; RNA, Viral2006
12 Margraf, Rebecca L.; Mao, Rong; Highsmith, W. Edward; Holtegaard, Leonard; Wittwer, Carl T.Masking selected sequence variation by incorporating mismatches into melting analysis probesHybridization probe melting analysis can be complicated by the presence of sequence variations (benign polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Selected sequence variati...probe hybridization; high-resolution melting analysis; masking; RET2005-06-28
13 Coffin, Cheryl M.; Capecchi, Mario R.Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function.Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has...Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors2004-11-01
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