| | Title | Creator | Description | Subject | Date |
|---|
| 1 |  | Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function: implications for alveolar rhabdomyosarcoma cell of origin. | Hansen, Mark S.; Coffin, Cheryl M.; Capecchi, Mario R. | To investigate the role of the translocation-associated gene Pax3:Fkhr in alveolar rhabdomyosarcomas, we generated a Cre-mediated conditional knock-in of Pax3:Fkhr into the mouse Pax3 locus. Exploring embryonic tumor cell origins, we replaced a Pax3 allele with Pax3:Fkhr throughout its expression do... | Neuroprogenitor; Embryogenesis | 2004-11-01 |
| 2 |  | Mouse model for the delta F508 allele of cystic fibrosis | Eichwald, Ernst; Capecchi, Mario R.; Thomas, Kirk R. | The most common cause of cystic fibrosis is a mutation that deletes phenylalanine 508 in cystic fibrosis transmembrane conductance regulator (CFTR). The delta F508 protein is misprocessed and degraded rather than traveling to the apical membrane. We used a novel strategy to introduce the delta F508 ... | Digestive System; Disease Models, Animal; Electrolytes; Mice, Inbred C57BL | 1995-10 |
| 3 |  | Alveolar rhabdomyosarcomas in conditional Pax3:Fkhr mice: cooperativity of Ink4a/ARF and Trp53 loss of function. | Coffin, Cheryl M.; Capecchi, Mario R. | Alveolar rhabdomyosarcoma is an aggressive childhood muscle cancer for which outcomes are poor when the disease is advanced. Although well-developed mouse models exist for embryonal and pleomorphic rhabdomyosarcomas, neither a spontaneous nor a transgenic mouse model of alveolar rhabdomyosarcoma has... | Cell Differentiation; Forkhead Transcription Factors; Myogenic Regulatory Factors | 2004-11-01 |
| 4 |  | Hepatitis C genotyping by denaturing high-performance liquid chromatography | Liew, Michael; Erali, Maria; Page, Sam; Hillyard, David R.; Wittwer, Carl T. | Determination of the hepatitis C virus (HCV) genotype for infected patients increasingly has become accepted as the standard of care. Genotype assignment helps in assessing disease prognosis and assists in establishing the appropriate duration of treatment. The great genetic diversity of HCV, with 1... | Base Sequence; 5' Untranslated Regions; Genotype; Hepacivirus; Molecular Sequence Data | 2004-01 |
| 5 |  | Heterozygote PCR product melting curve prediction | Dwight, Zachary Lawrence | Melting curve prediction of PCR products is limited to perfectly complementary strands. Multiple domains are calculated by recursive nearest-neighbor thermodynamics. However, the melting curve of an amplicon containing a heterozygous single nucleotide variant (SNV) after PCR is the composite of four... | | 2014-01-01 |
| 6 |  | Performance enhancement in the measurement of 5 endogenous steroids by LC-MS/MS combined with differential ion mobility spectrometry | Rockwood, Alan L. | Background: Challenges for steroid analysis by LC-MS/MS include low ionization efficiency, endogenous isobars with similar fragmentation patterns and chromatographic retention. Differential ion mobility spectrometry (DMS) provides an additional degree of separation prior to MS/MS detection, and show... | | 2014-01-01 |
| 7 |  | uAnalyze: web-based high-resolution DNA melting analysis with comparison to thermodynamic predictions | Dwight, Zachary Lawrence | Abstract-uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unproce... | | 2012-01-01 |
| 8 |  | Effects of hemoglobin (Hb) E and HbD traits on measurements of glycated Hb (HbA1c) by 23 methods. | Owen, William E.; Roberts, William L. | Glycohemoglobin (GHB), reported as hemoglobin (Hb) A1c, is a marker of long-term glycemic control in patients with diabetes and is directly related to risk for diabetic complications. HbE and HbDare the second and fourth most common Hb variants worldwide. We investigated the accuracy of HbA1c measur... | Glycohemoglobin; GHB; Glycated Hb; Glycemic control | 2008 |
| 9 |  | Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids | Owen, William E.; Rockwood, Alan L.; Roberts, William L.; Meikle, A. Wayne | Congenital adrenal hyperplasia is a group of autosomal recessive disorders caused by a deficiency of 1 of 4 enzymes required for the synthesis of glucocorticoids, mineralocorticoids, and sex hormones. Analysis of 11-deoxycortisol (11DC), 17-hydroxyprogesterone (17OHP), 17-hydroxypregnenolone (17O... | Congenital adrenal hyperplasia; Adrenal steroids; Tandem mass spectrometry assay | 2006 |
| 10 |  | Development and validation of an automated thawing and mixing workcell | Owen, William E.; Roberts, William L.; Ring, Terry Arthur | Working toward a goal of total laboratory automation, we are automating manual activities in our highest volume laboratory section. Because half of all specimens arriving in this laboratory section are frozen, we began by developing an automated workcell for thawing frozen specimens and mixing the t... | Automated workcell; Laboratory automation | 2007 |
| 11 |  | Nucleic acid vaccine encoding gD2 protects mice from herpes simplex virus type 2 disease. | Kriesel, John D.; Spruance, Spotswood L.; Daynes, Raymond A. | Nucleic acid vaccinations with plasmids pWW65, containing the sequence for herpes simplex type 2 (HSV-2) gD2, and pRSVnt, lacking the gD sequence, were studied. Groups of mice were immunized with pWW65 alone, pWW65 plus 1,25-dihydroxyvitamin-D3 (D3), or pRSVnt. Clinical disease (vaginitis), serum an... | Mice, Inbred BALB C; Plasmids; Viral Vaccines | 1996 |
| 12 |  | Diagnostic performance of phospholipid-specific assays for the evaluation of antiphospholipid syndrome | Jaskowski, Troy D. | The diagnostic performance of commercially available nonstandard antiphospholipid (aPL) assays for the evaluation of antiphospholipid syndrome (APS) is unknown. In 62 patients with APS, 88 with recurrent pregnancy loss, 50 healthy blood donors, and 24 women with one or more successful pregnancies, w... | APS; Phospholipid-specific assays; Diagnostic performance | 2008-06-01 |
| 13 |  | Screening for antinuclear antibodies by enzyme immunoassay | Jaskowski, Troy D. | Indirect fluorescent antibody (IFA) is the most widely used method in clinical laboratories to screen for autoantibodies against a wide variety of nuclear antigens. Recently, a number of antinuclear antibody (ANA) enzyme immunoassay (EIA) screens have become commercially available and claim to be an... | Antinuclear antibodies; Enzyme immunoassay | 1996 |
| 14 |  | Comparison of four enzyme immunoassays with a western blot assay for the determination of type-specific antibodies to herpes simplex virus | Jaskowski, Troy D. | Most current enzyme immunoassays (EIAs) differentiate inadequately between types 1 and 2 herpes simplex virus (HSV) antibodies since significant crossreactivity exists. We compared 4 IgG type-specific EIAs using a Western blot assay for resolution of discrepant results. The Diamedix had sensitivit... | Enzyme immunoassays; Western blot assay; Type-specific antibodies | 2001 |
| 15 |  | Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay results | Jaskowski, Troy D. | We hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measur... | Heterophil antibodies; Heterophil interference; Multiplex analysis; False-positive HIV enzyme-linked immunosorbent assay; ELISA | 2001 |
| 16 |  | Do dentists prescribe narcotics excessively? | Aldous, Jay A.; Engar, Richard C. | Dealing with pain is an inevitable sequela to dental treatment. Although several drug regimens primarily involving narcotics have been used in the past, availability of nonsteroidal anti-inflammatory drugs (NSAIDs) has increased recently. A study was conducted to analyze dental prescribing patterns ... | Analgesics, Opioid; Anti-Inflammatory Agents; Dentist's Practice Patterns; Pain, Postoperative | 1996-07-01 |
| 17 |  | Ultrasonic enhancement of antibiotic action on several species of bacteria | Hymas, Weston C. | The effect of the antibiotics gentamicin, streptomycin, kanamycin, tetracycline, and ampicillin on planktonic cultures of Enterobacter aerogenes, Serratia marcescens, Salmonella derby, Streptococcus mitis, and Staphylococcus epidermidis with and without an application of 70 kHz ultrasound was studi... | Enterobacter aerogenes; Serratia marcescens; Salmonella derby; Streptococcus mitis; Staphylococcus epidermidis; Planktonic cultures; Gentamicin; Streptomycin; Kanamycin; Tetracycline; Ampicillin; Ultrasonic enhancement; Bioacoustic effect | 1998 |
| 18 |  | Masking selected sequence variation by incorporating mismatches into melting analysis probes | Margraf, Rebecca L.; Mao, Rong; Highsmith, W. Edward; Holtegaard, Leonard; Wittwer, Carl T. | Hybridization probe melting analysis can be complicated by the presence of sequence variations (benign polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Selected sequence variati... | probe hybridization; high-resolution melting analysis; masking; RET | 2005-06-28 |
| 19 |  | Effects of hemoglobin C and S traits on the results of 14 commercial glycated hemoglobin assays | Owen, William E.; Roberts, William L. | Glycated hemoglobin is widely used in the management of diabetes mellitus. At least 300,000 Americans with diabetes mellitus have the hemoglobin (Hb) C or S trait. The accuracy of HbA1c methods can be adversely affected by the presence of these traits. We evaluated the effects of HbC and HbS traits ... | Glycated hemoglobin assays; Hemoglobin C trait; Hemoglobin S trait | 2008 |
| 20 |  | Effects of calcium ions on L-type horizontal cells in the isolated turtle retina | Normann, Richard A.; Chandler, John P. | A technique by which the retina can be isolated from the turtle eye is described. Scanning electron microscopy revealed morphological variability between preparations and also between regions of the same one. Large areas were often totally free of any pigment epithelial cells, yet contained a high p... | Retina; Calcium; Horizontal Cell; Turtle | 1990 |
| 21 |  | Evaluation of a PCR probe capture assay for the detection of Toxoplasma gondii: incorporation of uracil N-glycosylase for contamination control | Jaskowski, Troy D. | Toxoplasma gondii is a cyst-forming parasite of clinical relevance in humans primarily because of the neurologic abnormalities it can cause. In some clinical circumstances, it is desirable to detect the pathogen directly. We modified a commercially available Toxoplasma polymerase chain reaction... | PCR probe capture assay; Uracil N-glycosylase; UNG | 2000 |
| 22 |  | Performance characteristics of six IMMULITE 2000 TORCH assays | Owen, William E.; Martins, Thomas B.; Litwin, Christine M.; Roberts, William L. | TORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (like syphilis), rubella (RV), cytomegalovirus (CMV), and herpesviruses (HSV) that are associated with congenital abnormalities during maternal infection. Prenatal testing for antibodies against these agents is one tool for preve... | TORCH; Toxoplasma gondii; syphilis virus; rubella; cytomegalovirus; herpesvirus; IMMULITE 2000; congenital abnormalities; laboratory testing | 2006-01-04 |
| 23 |  | Humananti-mouse antibody protected ELISA for the quantification of squamous cell carcinoma antigen | Erickson, J. Alan; Smith, Jeffrey, J.; Bornhorst, Joshua A.; Ashwood, Edward R. | Squamous cell carcinoma antigen (SCC) was first isolated from squamous cell carcinoma tissue of the uterine cervix and initially reported as TA-4. The antigen has been characterized as a glycoprotein with a molecular weight between 45,000 and 55,000 daltons. Studies suggest that SCC may function as ... | SCC; Squamous Cell Carcinoma Antigen; BSA; PBS; Phosphate Buffered Saline; HAMA; Human Anti-mouse Antibodies; IIR; ImmunoglobulinInhibiting Reagent | 2005-12-09 |
| 24 |  | Masking techniques: masking selected sequence variation by incorporating mismatches into melting analysis probes | Margraf, Rebecca L.; Mao, Rong; Wittwer, Carl T. | Hybridization probe melting analysis can be complicated by the presence of sequence variation (non-pathogenic polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Materials and Meth... | probe hybridization; high-resolution melting analysis; masking; RET | 2005-10-21 |
| 25 |  | Mass spectrometry for steroids, immunosuppressants, and other clinical applications | Rockwood, Alan L. | Presentation slides on matching mass spectrometry technology to clinical applications. | Immunosuppressants | 2005-07-26 |
