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Herrmann, Mark G.; Durtschi, Jacob; Bromley, L. Kathryn; Voelkerding, Karl V. | Cross platform comparison of melting curve analysis | Melting analysis of PCR product was first performed on the LightCycler 10 years ago. Now, melting analysis is a standard function on all real-time PCR instruments. Recent advances in DNA melting analysis, including high resolution melting and specialized dyes, have increased the capabilities of melt... | PCR; SNP; SYBR Green I; LC Green Plus; high-resolution melting; mutation scanning; amplicon genotyping | 2005-11-04 |
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Pounder, June I.; Voelkerding, Karl V.; Storts, D.; Anderson, Clint M. | Differentiation of species within mycobacterium tuberculosis complex by real-time PCR-based genomic deletion analysis | Differentiation of members within Mycobacterium tuberculosis complex (MTC) is essential for patient management and infection control. MTC is comprised of M. tuberculosis (Mtb), M. bovis (Mb), M. bovis BCG (vaccine strain; Mbcg), M. africanum (Ma), and M. microti (Mm). Because MTC have highly similar... | MTC; species differentiation; Mycobacterium bovis BCG; Mycobacterium africanum; Mycobacterium microtic; PCR | 2006-01-09 |