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CreatorTitleDescriptionSubjectDate
51 Baehr, Wolfgang; Zhang, KangElovl4 mRNA distribution in the developing mouse retina and phylogenetic conservation of Elovl4 genesPURPOSE: Stargardt-like macular dystrophy (STGD3) is an autosomal dominant form of early onset macular degeneration. The disease causing gene ELOVL4 encodes a protein that belongs to a family of proteins functioning in elongation of long chain fatty acids. The purpose of this study is to characteriz...Amino Acid Sequence; Cloning, Molecular; Molecular Sequence Data2003
52 Normann, Richard A.; Shoham, ShyEncoding mechanisms for sensory neurons studied with a multielectrode array in the cat dorsal root ganglionRecent advances in microelectrode array technology now permit a direct examination of the way populations of sensory neurons encode information about a limb's position in space. To address this issue, we recorded nerve impulses from about 100 single units simultaneously in the L6 and L7 dorsal root ...Sensory; Encoding; Multielectrode; Dorsal Root Ganglion; Cutaneour; Muscle2004
53 Baehr, WolfgangEvaluation of the 17 kda prenyl binding protein as a regulatory protein for phototransduction in retinal photoreceptorsThe mammalian rod photoreceptor phosphodiesterase (PDE6) holoenzyme is isolated in both a membrane-associated and a soluble form. Membrane binding is a consequence of prenylation of PDE6 catalytic subunits, whereas soluble PDE6 is purified with a 17-kDa prenyl-binding protein (PDEdelta) tightly boun...Amino Acid Sequence; Immunohistochemistry; Phosphoric Diester Hydrolases2005
54 Bernstein, Paul S.Evaluation of the G protein coupled receptor-75 (GPR75) in age related macular degeneration2001
55 Angelucci, AlessandraExperimentally induced retinal projections to the ferret auditory thalamus: development of clustered eye-specific patterns in a novel targetWe have examined the relative role of afferents and targets in pattern formation using a novel preparation, in which retinal projections in ferrets are induced to innervate the medial geniculate nucleus (MGN). We find that retinal projections to the MGN are arranged in scattered clusters. Clusters a...Retina; Ferrets1997
56 Baehr, WolfgangExpression and mutagenesis of mouse rod photoreceptor cGMP phosphodiesteraseUsing recombinant baculovirus vectors, the three subunits of mouse rod photoreceptor cGMP phosphodiesterase (PDE) (alpha beta gamma 2) have been expressed in insect cells. The recombinant alpha,beta subunits accumulate to 5 mg/liter culture, but most (98%) of the expressed polypeptides are insoluble...Electrophoresis, Polyacrylamide Gel; Macromolecular Substances; Protein Processing, Post-Translational1994
57 Fuhrmann, SabineExpression of CNTF receptor-alpha in chick violet-sensitive cones with unique morphologic properties.PURPOSE: Application of ciliary neurotrophic factor (CNTF) can rescue mature photoreceptors from lesion-induced and hereditary degeneration. In the chick retina, expression of the CNTF receptor is present in a subpopulation of photoreceptor cells. The present study was undertaken to identify the CNT...embryology; cytology2004
58 Zhang, KangExpression of wild type and mutant ELOVL4 in cell culture: subcellular localization and cell viabilityPURPOSE: ELOVL4 is a member of the fatty acid elongase (ELO) family of genes. Mutations of this gene are responsible for autosomal dominant Stargardt-like macular degeneration. However, the specific role of ELOVL4 in photoreceptor cells and the mechanism by which mutations in ELOVL4 causes macular d...Endoplasmic Reticulum; Green Fluorescent Proteins; Microscopy, Fluorescence2004
59 Fuhrmann, SabineExtraocular ectoderm triggers dorsal retinal fate during optic vesicle evagination in zebrafishDorsal retinal fate is established early in eye development, via expression of spatially restricted dorsal‐specific transcription factors in the optic vesicle; yet the events leading to initiation of dorsal fate are not clear. We hypothesized that induction of dorsal fate would require an extraocu...2012-01-01
60 Jones, Bryan W.; Jones, Christopher R.; Czajkowski, LauraFamilial advanced sleep-phase syndrome: a short-period circadian rhythmvariant in humansBiological circadian clocks oscillate with an approximately 24-hour period, are ubiquitous, and presumably confer a selective advantage by anticipating the transitions between day and night. The circadian rhythms of sleep, melatonin secretion and body core temperature are thought to be generated by ...Activity cycles; matched-pair analysis; polysomnography1999
61 Normann, Richard A.Functional reorganization of primary visual cortex induced by electrical stimulation in the cat.Compared to the high degree of plasticity observed in a juvenile, mature sensory cortices have long been held to be immutable but, recently, researchers have suggested some plasticity persists in the mature cortex. Cortical reorganization has particular saliency to the development of a cortically ba...Cat; Electrophysiology; Phosphene; Plasticity; Receptive Field; Striate Cortex2005
62 Baehr, Wolfgang; Zhang, Kang; Kolb, HelgaGene array and expression of mouse retina guanylate cyclase activating proteins 1 and 2PURPOSE: To identify gene arrangement, chromosomal localization, and expression pattern of mouse guanylate cyclase activating proteins GCAP1 and GCAP2, retina-specific Ca2+-binding proteins, and photoreceptor guanylate cyclase activators. METHODS: The GCAP1 and GCAP2 genes were cloned from genomic l...Molecular Sequence Data; Guanylate Cyclase-Activating Proteins; In Situ Hybridization, Fluorescence1998
63 Bernstein, Paul S.; Leppert, Mark F.Genotype-phenotype analysis of ABCR variants in macular degeneration probands and siblingsPURPOSE: Single-copy variants of the autosomal recessive Stargardt disease (STGD1) gene ABCR (ABCA4) have been shown to confer enhanced susceptibility to age-related macular degeneration (AMD). To investigate the role of ABCR alleles in AMD further, genotype-phenotype analysis was performed on sibli...DNA Mutational Analysis; Nuclear Family; Phenotype2002
64 Olson, Randall J.Glistenings with long-term follow-up of the Surgidev B20/20 polymethylmethacrylate intraocular lensPURPOSE: To compare patient satisfaction with vision 12 months after implantation of a silicone or acrylic foldable intraocular lens (IOL). SETTING: John A. Moran Eye Center, University of Utah Medical Center, Salt Lake City, Utah, USA. METHODS: Selection criteria included patients who had uneventfu...Phacoemulsification; Lens Implantation, Intraocular; Glare2001-10
65 Baehr, WolfgangGuanylyl cyclase activating protein. A calcium-sensitive regulator of phototransductionGuanylyl cyclase activating protein (GCAP1) has been proposed to act as a calcium-dependent regulator of retinal photoreceptor guanylyl cyclase (GC) activity. Using immunocytochemical and biochemical methods, we show here that GCAP1 is present in rod and cone photoreceptor outer segments where photo...Cloning, Molecular; DNA Primers; Molecular Sequence Data1995
66 Kriesel, John D.Helicase-primase inhibitor pritelivir for HSV-2 infectionBackground Pritelivir, an inhibitor of the viral helicase-primase complex, exhibits antiviral activity in vitro and in animal models of herpes simplex virus (HSV) infection. We tested the efficacy and safety of pritelivir in otherwise healthy persons with genital HSV-2 infection. Methods We randomly...2014-01-01
67 Zhang, KangHeterozygous and homozygous mutations in PITX3 in a large Lebanese family with posterior polar cataracts and neurodevelopmental abnormalitiesPURPOSE: The PITX3 gene, which codes for a homeobox bicoidlike transcription factor is responsible for dominant cataract and anterior segment mesenchymal dysgenesis in humans. In the current study, a family with autosomal dominant posterior polar cataract (PPC) and a PITX3 mutation that cosegregates...Chromosomes, Human, Pair 10; Homozygote; Lod Score2006
68 Bernstein, Paul S.HPLC measurement of ocular carotenoid levels in human donor eyes in the lutein supplementation era.PURPOSE: A substantial proportion of the population at risk for visual loss from age-related macular degeneration consumes supplements containing high doses of lutein, but clinical studies to date have shown only modest and variable increases in macular carotenoid pigments in response to supplementa...Chromatography, High Pressure Liquid; Dietary Supplements; Tissue Donors2007-02
69 Baehr, WolfgangHuman interstitial retinoid-binding protein. Gene structure and primary structureInterstitial retinoid-binding protein (IRBP) is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix and is known to bind retinoids and fatty acids. We have used cDNA clones encoding human IRBP to isolate a 15-kilobase genomic fragment that encompasses the complete ...Molecular Sequence Data; Eye Proteins; Retinol-Binding Proteins; Messenger RNA1989-05-15
70 Bernstein, Paul S.Human retinal pigment epithelial cell line that retains epithelial characteristics after prolonged culturePURPOSE. A spontaneously arising, apparently transformed, cell line has been cloned from a primary culture of human retinal pigment epithelial (RPE) cells and has been subcultured more than 200 times. The similarities of these cells to human RPE cells in vivo have been determined. METHODS. The struc...Cell Line, Transformed; Chromatography, High Pressure Liquid; Phagocytosis1995
71 Frederick, Jeanne M.; Thulin, Craig; Bernstein, Paul S.Identification and characterization of a Pi isoform of glutathione S-transferase (GSTP1) as a zeaxanthin-binding protein in the macula of the human eyeUptake, metabolism, and stabilization of xanthophyll carotenoids in the retina are thought to be mediated by specific xanthophyll-binding proteins (XBPs). A membrane-associated XBP was purified from human macula using ion-exchange chromatography followed by gel-exclusion chromatography. Two-dimensio...Circular Dichroism; Isoelectric Focusing; Macula Lutea; Xanthophylls2004
72 Zhang, KangIdentification and functional consequences of a new mutation (E155G) in the gene for GCAP1 that causes autosomal dominant cone dystrophy2001
73 Baehr, WolfgangIdentification and light-dependent translocation of a cone-specific antigen, (Cone Arrestin) recognized by monoclonal antibody 7G6PURPOSE: To elucidate the antigen recognized by monoclonal antibody (mAb) 7G6, a widely used cone-specific marker. METHODS: 7G6 immunocytochemistry was performed on sections of human, primate, and bovine retina. The antigen was immunoprecipitated from human retinal lysates and purified with protein ...Cones (Retina); Molecular Sequence Data; Phototransduction2003
74 Crockett, David K.Identification of histoplasma -specific peptides in human urineHistoplasmosis is a severe dimorphic fungus infection, which is often difficult to diagnose due to similarity in symptoms to other diseases and lack of specific diagnostic tests. Urine samples from histoplasma-antigen-positive patients and appropriate controls were prepared using various sample prep...2012-01-01
75 Bernstein, Paul S.Identification of lutein and zeaxanthin oxidation products in human and monkey retinasPURPOSE: To characterize fully all the major and minor carotenoids and their metabolites in human retina and probe for the presence of the oxidative metabolites of lutein and zeaxanthin. METHODS: Carotenoids of a composite of 58 pairs of human retinas and a monkey retina were elucidated by comparing...Mass Spectrometry; Oxidation-Reduction; Spectrophotometry, Ultraviolet1997
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