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| Creator | Title | Description | Subject | Date | ||
|---|---|---|---|---|---|---|
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Gesteland, Raymond F.; Atkins, John F.; Wills, Norma M. | rRNA-mRNA base pairing stimulates a programmed -1 ribosomal frameshift | Base pairing between the 3' end of 16S rRNA and mRNA is shown to be important for the programmed -1 frameshifting utilized in decoding the Escherichia coli dnaX gene. This pairing is the same as the Shine-Dalgarno pairing used by prokaryotic ribosomes in selection of translation initiators, but for ... | Frameshifting; Escherichia coli; Binding Sites; Base Composition | 1994 |
| 2 |
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Gesteland, Raymond F.; Atkins, John F.; Ingram, Jennifer A.; Kelly, Paul J.; Grentzmann, Guido | Dual-luciferase reporter system for studying recoding signals | A new reporter system has been developed for measuring translation coupling efficiency of recoding mechanisms such as frameshifting or readthrough. A recoding test sequence is cloned in between the renilla and firefly luciferase reporter genes and the two luciferase activities are subsequently measu... | Amino Acid Sequence; Genes, Reporter; HIV; Antizyme; Translation | 1998 |
| 3 |
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Capecchi, Mario R. | Establishment of mammalian cell lines containing multiple nonsense mutations and functional suppressor tRNA genes. | We describe the generation of mammalian cell lines carrying amber suppressor genes. Nonsense mutants in the herpes simplex virus thymidine kinase (HSV tk) gene, the Escherichia coli xanthine-guanine phosphoribosyl transferase (Eco-gpt) gene and the aminoglycoside 3' phosphotransferase gene of the Tn... | Cercopithecus aethiops; Escherichia coli; Xenopus | 1982-11-30 |
| 4 |
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Gesteland, Raymond F.; Atkins, John F. | Influence of the stacking potential of the base 3' of tandem shift codons on -1 ribosomal frameshifting used for gene expression | Translating ribosomes can shift reading frame at specific sites with high efficiency for gene expression purposes. The most common type of shift to the 1 frame involves a tandem realignment of two anticodons from pairing with mRNA sequence of the form X XXY YYZ to XXX YYY Z where the spaces indicate... | 1 frameshifting; 39 context effect; Codon anticodon interaction; Escherichia coli ; Recoding; tRNALys; XXXY YYZ frameshift motifs | 2002 |
| 5 |
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Capecchi, Mario R. | Purification and characterization of mouse hypoxanthine-guanine phosphoribosyltransferase. | Hypoxanthine-guanine phosphoribosyltransferase (HGPR transferase) (EC 2.4.2.8) has been purified approximately 4500-fold to apparent homogeneity from mouse liver. The procedure involves the use of affinity chromatography and was designed to be readily adaptable to small scale isolations. The enzyme ... | Buffers; Centrifugation, Density Gradient; Chromatography, Affinity; Chromatography, Gel; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel | 1975-01-31 |
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