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CreatorTitleDescriptionSubjectDate
1 Poulter, Charles DaleIsolation of Schizosaccharomyces pombe isopentenyl diphosphate isomerase cDNA clones by complementation and synthesis of the enzyme in Escherichia coliIsopentenyl diphosphate (IPP) isomerase catalyzes an essential activation step in the isoprene biosynthetic pathway. The Saccharomyces cerevisiae gene for IPP isomerase, IDI1, was recently isolated and characterized (Anderson, M. S., Muehlbacher, M., Street, I. P., Proffitt, J., and Poulter, C. D. (...Carbon-Carbon; Biosynthesis; Genetics1995-05-12
2 Voth, Gregory Alan; Ayton, Gary S.Role of protein interactions in defining HIV-1 viral capsid shape and stability: a coarse-grained analysisCoarse-grained models of the HIV-1 CA dimer are constructed based on all-atom molecular dynamics simulations. Coarse-grained representations of the capsid shell, which is composed of approximately 1,500 copies of CA proteins, are constructed and their stability is examined. A key interaction between...Virus capsid; HIV-1; Viral capsid shape; Coarse-graining; CA dimer2010-01
3 Poulter, Charles DaleIsopentenyl diphosphate:dimethylallyl diphosphate isomerase. An improved purification of the enzyme and isolation of the gene from Saccharomyces cerevisiae.Isopentenyl diphosphate:dimethylallyl diphosphate isomerase (IPP isomerase) is an enzyme in the isoprenoid biosynthetic pathway which catalyzes the interconversion of the primary five-carbon homoallylic and allylic diphosphate building blocks. We report a substantially improved procedure for purific...Amino Acid Sequence; Base Sequence; Chromatography, Gel; Chromatography, Ion Exchange1989-11-15
4 Poulter, Charles DaleLethal mutations in the isoprenoid pathway of salmonella entericaEssential isoprenoid compounds are synthesized using the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway in many gram-negative bacteria, some gram-positive bacteria, some apicomplexan parasites, and plant chloroplasts. The alternative mevalonate pathway is found in archaea and eukaryotes, includin...Isoprenoids; Mutation; Bacteria; Antimicrobials2006
5 Poulter, Charles DaleFarnesyl diphosphate synthetase: molecular cloning, sequence, and expression of an essential gene from Saccharomyces cerevisiaeFarnesyl diphosphate (FPP) synthetase is a key enzyme in isoprenoid biosynthesis which supplies C15 precursors for several classes of essential metabolites including sterols, dolichols, and ubiquinones. The structural gene for FPP synthetase was isolated on a 4.5-kilobase EcoRI genomic restriction f...Genetics; Transferases; Dimethylallyltranstransferase/biosynthesis1989-11-15
6 Poulter, Charles DaleEnzymes encoded by the farnesyl diphosphate synthase gene family in the big sagebrush Artemisia tridentata ssp. spiciformisFarnesyl diphosphate synthase catalyzes the sequential head-to-tail condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate. In plants the presence of farnesyl diphosphate synthase isozymes offers the possibility of differential regulation. Three full-length cDNAs enc...Amino Acid Sequence; Base Sequence; Molecular Sequence Data2003-08-22
7 Poulter, Charles DaleIsoprenyl diphosphate synthases: protein sequence comparisons, a phylogenetic tree, and predictions of secondary structureIsoprenyl diphosphate synthases are ubiquitous enzymes that catalyze the basic chain-elongation reaction in the isoprene biosynthetic pathway. Pairwise sequence comparisons were made for 6 farnesyl diphosphate synthases, 6 geranylgeranyl diphosphate synthases, and a hexaprenyl diphosphate synthase...Catalytic site; Evolution; Farnesyl diphosphate; Geranylgeranyl diphosphate; Prenyltransferase; Substrate binding:1994
8 Miller, Joel Steven; Epstein, Arthur J.Local structural order in the disordered vanadium tetracyanoethylene room-temperature molecule-based magnetWe determined the vanadium oxidation state and local coordination environment in disordered samples of magnetic V[TCNE]x(x=2) prepared by chemical vapor deposition (CVD). Systematic studies of the x-ray absorption near-edge structure (XANES) in this material and reference compounds show that V ions ...Magnetic; Solvent; Structure2004
9 Poulter, Charles DaleCaaX proteases, Afc1p and Rce1p, have overlapping but distinct substrate specificitiesMany proteins that contain a carboxyl-terminal CaaX sequence motif, including Ras and yeast a-factor, undergo a series of sequential posttranslational processing steps. Following the initial prenylation of the cysteine, the three C-terminal amino acids are proteolytically removed, and the newly fo...Proteins; Enzymes; Hydrophobic2000
10 Morse, Michael DavidSpectroscopy and electronic structure of jet-cooled GaAsAn optical spectrum, obtained by resonant two-photon ionization spectroscopy, is reported for jet-cooled diatomic gallium arsenide. The ground state is identified as X??-, deriving from a ??rr? molecular configuration, and is characterized by ?" = 215 cm-1, ?"exe" = 3 cm-1, and r0' = 2.53 ? 0.02 ?. ...1989
11 Morse, Michael DavidSpectroscopy of AlAr and AlKr from 31 000 cm-1 to the ionization limitSpectra of jet-cooled AlAr and AlKr have been collected over the range from 31 000 cm -? to the ionization limit, using the technique of resonant two-photon ionization spectroscopy with mass spectrometric detection. Adiabatic ionization potentials have been accurately measured for both molecules, an...1992
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