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Margraf, Rebecca L.; Mao, Rong; Wittwer, Carl T. | Masking techniques: masking selected sequence variation by incorporating mismatches into melting analysis probes | Hybridization probe melting analysis can be complicated by the presence of sequence variation (non-pathogenic polymorphisms or other mutations) near the targeted mutation. We investigated the use of 'masking' probes to differentiate alleles with similar probe melting temperatures. Materials and Meth... | probe hybridization; high-resolution melting analysis; masking; RET | 2005-10-21 |
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