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Creator | Title | Description | Subject | Date |
101 |
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Capecchi, Mario R.; Bernstein, Kenneth E.; Thomas, Kirk R. | Targeting genes for self-excision in the germ line | A procedure is described that directs the self-induced deletion of DNA sequences as they pass through the male germ line of mice. The testes-specific promoter from the angiotensin-converting enzyme gene was used to drive expression of the Cre-recombinase gene. Cre was linked to the selectable marker... | Cre-recombinase; Hoxa3; Chimeric mice | 1999-06-15 |
102 |
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Baehr, Wolfgang | Tissue-specific expression in transgenic mice directed by the 5'-flanking sequences of the human gene encoding interphotoreceptor retinoid-binding protein | Interphotoreceptor retinoid-binding protein (IRBP) is an extracellular protein that has been suggested to participate in the visual process as a carrier for visual retinoids. A chimeric gene composed of the human IRBP promoter fused to the bacterial reporter gene chloramphenicol acetyltransferase (C... | Restriction Mapping; Nucleic Acid Hybridization; Mice; Gene Expression | 1990-05-25 |
103 |
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Gesteland, Raymond F. | Translation of Rl7 RNA fragments | Examination of the events during infection of cells by RNA phages reveals phemonomena that are surprisingly complex for a virus that has only enough information to code for three to four proteins. The coat protein is synthesized at a rapid rate through most of the infectious cycle making it the pre... | Electrophoresis; RNA, Messenger; Peptide Biosynthesis; Kinetics | 1969 |
104 |
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Gesteland, Raymond F.; Atkins, John F.; Tuohy, Therese M. | Uninterrupted translation through putative 12-nucleotide coding gap in sequence of carA: business as usual | Previous work of others reported an untranslated stretch of 12 nucleotides in the 5' coding sequence of carA from Pseudomonas aeruginosa. However, N-terminal protein sequencing of carA-lacZ translational fusions shows that these 12 nucleotides are normally translated in a continuous triplet manner, ... | Base Sequence; Protein Biosynthesis; Sequence Deletion | 1994 |
105 |
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Hansen, Mark S.; Healy, Lindsey J.; Johnson, Christopher R.; Capecchi, Mario R.; Keller, Charles; Jones, Greg M. | Virtual histology of transgenic mouse embryos for high-throughput phenotyping. | A bold new effort to disrupt every gene in the mouse genome necessitates systematic, interdisciplinary approaches to analyzing patterning defects in the mouse embryo. We present a novel, rapid, and inexpensive method for obtaining high-resolution virtual histology for phenotypic assessment of mouse ... | Forkhead Transcription Factors; Paired Box Transcription Factors | 2006 |
106 |
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Capecchi, Mario R. | Xanthine oxidoreductase is central to the evolution and function of the innate immune system. | The housekeeping enzyme xanthine oxidoreductase (XOR) has been studied intensively over the past 100 years, yet the complexity of its in vivo function is still poorly understood. A large body of literature focuses on the different catalyltic forms of XOR and their importance in the synthesis of reac... | Animals; Gene Expression Regulation, Enzymologic; Humans; Immune System; Models, Biological | 2003-09-01 |
107 |
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Capecchi, Mario R. | Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro. | tRNA isolated from two different yeast super-suppressor strains translates a known nonsense mutation in vitro, whereas tRNA from a closely related nonsuppressing strain does not. Suppression was assayed by translation of RNA isolated from an amber coat mutant of bacteriophage Qbeta (GB11) in a prote... | Codon; Escherichia coli; Protein Biosynthesis | 1975-11 |