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451 Hansen, Mark S.; Coffin, Cheryl M.; Capecchi, Mario R.Pax3:Fkhr interferes with embryonic Pax3 and Pax7 function: implications for alveolar rhabdomyosarcoma cell of origin.To investigate the role of the translocation-associated gene Pax3:Fkhr in alveolar rhabdomyosarcomas, we generated a Cre-mediated conditional knock-in of Pax3:Fkhr into the mouse Pax3 locus. Exploring embryonic tumor cell origins, we replaced a Pax3 allele with Pax3:Fkhr throughout its expression do...Neuroprogenitor; Embryogenesis2004-11-01
452 Sekercioglu, CaganPeople skills for the conservation professionalConservation scientists are increasingly becoming aware of the importance of the human aspects of conservation, and in conservation circles, ‘‘social science'' is the word of the day. There is a rapidly growing social science working group in the Society for Conservation Biology and it is hard t...2008-01-01
453 Olivera, Baldomero M.; Gray, William RobertPeptide toxins from Conus geographus venomThe biochemical characterization of three highly toxic peptides from the venom of the marine snail Conus geographus is described in this report. These peptides cause their potent activity by inhibition of the postsynaptic terminus of the vertebrate neuromuscular junction. The relatively small size o...Conus geographus; Venom; Conotoxins; Neurotoxins1981
454 Capecchi, Mario R.Personal view of gene targetingGene targeting provides the means for creating strains of mice with mutations in virtually any gene.1 First, the desired mutation is introduced into a cloned copy of the chosen gene by standard recombinant DNA technology. The mutation is then transferred to the genome of a pluripotent mouse embryo-d...1995
455 Coley, Phyllis D.; Kursar, Thomas A.Pests vs. drought as determinants of plant distribution along a tropical rainfall gradientUnderstanding the mechanisms that shape the distribution of organisms can help explain patterns of local and regional biodiversity and predict the susceptibility of communities to environmental change. In the species-rich tropics, a gradient in rainfall between wet evergreen and dry seasonal forests...Drought tolerance; Herbivory; Panama; Pathogen attack; Rainfall gradient; Tree distribution; Tropical forests2009
456 Hughes, Kelly T.Phase variation in Salmonella: analysis of Hin recombinase and hix recombination site interaction in vivoThe bacteriophage P22-based challenge phase selection was used to characterize the binding of Salmonella Hin recombinase to the wild-type hixL and hixR recombination sites, as well as to mutant and synthetic hix sequences in vivo. Hin recombinase binds to the hixL or hixR recombination sites and rep...1988
457 Bohs, Lynn A.Phylogenetic relationships among the "spiny solanums" (Solanum subgenus Leptostemonum, Solanaceae)Species of Solanum subgenus Leptostemonum comprise almost one third of the genus and are distributed worldwide. Members of this group are defined by their sharp epidermal prickles; thus, they are commonly referred to as the ˜spiny solanums." This subgenus includes a number of economically important...Granule-bound starch synthase gene; GBSSI; Heterandry; ITS; Leptostemonum; Solanum; trnS-trnG; Waxy2006
458 Bohs, Lynn A.Phylogenetic relationships in Solanum (Solanaceae) based on ndhF sequencesA phylogenetic analysis was conducted using sequence data from the chloroplast gene ndhF. Sequences were obtained from 25 species of Solanaceae, including 18 species of Solanum representing five of the seven conventionally recognized subgenera.Solanum; ndhF1997
459 Bohs, Lynn A.Phylogenetic relationships in solanum section Androceras (Solanaceae)The Leptostemonum clade of Solanum contains approximately 350-450 species, including the cultivated eggplant, S. melongena. This clade is characterized by the presence of prickles and apically attenuate anthers. Solanum section Androceras, the focus of this study, is a group of ca. 12 species belon...2010-12-01
460 Bohs, Lynn A.Phylogeny and biogeography of the Canarian Solanum vespertilio and S. lidii (Solanaceae)The endemic and rare Solanum vespertilio and S. lidii from the Canary Islands are notable in being andromonoecious, self compatible, highly heterandrous (with short >reward= anthers and one very long >pollination= anther), and bearing strongly zygomorphic corollas. Solanum vespertilio flowers are a...Solanum vespertilio; Solanum lidii; Leptostemonum; tnT-F region; granule-bound starch synthase gene; GBSSI; Andromonoecy; Hererandry; Enantiostyly2006
461 Bohs, Lynn A.Phylogeny and provisional classification of the Solanaceae based on chloroplast DNAA phylogenetic analysis of the Solanaceae based on chloroplast DNA variation is presented. Restriction site maps were determined for 79 species (52 genera) for 11 restriction enzymes.rbcL; ndhF; Chloroplast DNA1999
462 Bohs, Lynn A.Phylogeny of Balsamorhiza and Wyethia (Asteraceae: Heliantheae) using ITS, ETS, and trnK sequence dataBalsamorhiza and Wyethia together comprise 24 species native to western North America. All species in the two genera are perennial herbs with large taproots and chromosome base numbers of x = 19. The species of Balsamorhiza have exclusively basal leaves while the species of Wyethia have cauline leav...Balsamorhiza; Wyethia; Asteraceae; Engelmanniinae; nuclear ribosomal DNA2007
463 Bohs, Lynn A.Phylogeny of the carolinense clade of solanum (Solanaceae) inferred from nuclear and plastid DNA sequencesThe large and economically important genus Solanum contains ca. 1,400 species distributed worldwide. One of the 12-14 major clades identified in the genus is the Leptostemonum clade, or the "spiny solanums." Previous molecular phylogenetic studies have identified 14 major clades in the spiny solanum...2014-01-01
464 Bohs, Lynn A.Phylogeny of the Cyphomandra clade of the genus Solanum (Solanaceae) based on ITS sequence data13 major clades can be recognized within the genus Solatium (Solanaceae) based on chloroplast DNA sequence data. One of these is the Cyphomandra clade, which includes about 50 neotropical species. These have traditionally been placed into two or three sections: S. section Pachyphylla (formerly recog...Cyphomandra; ITS; osmophores; self-incompatibility; Solanum2007
465 Goller, FranzPhysiologically driven avian vocal synthesizerIn this work, we build an electronic syrinx, i.e., a programmable electronic device capable of integrating biomechanical model equations for the avian vocal organ in order to synthesize song. This vocal prosthesis is controlled by the bird's neural instructions to respiratory and the syringeal mot...2010-03
466 Ehleringer, James R.Plant adaptation in the Great Basin and Colorado PlateauAdaptive features of plants of the Great Basin are reviewed. The combination of cold winters and an arid to semiarid precipitation regime results in the distinguishing features of the vegetation in the Great Basin and Colorado Plateau. The primary effects of these climatic features arise from how t...Plant adaptation; Great Basin; Colorado Plateau; Cold deserts1992
467 Adler, Frederick R.Plant signalling: The opportunities and dangers of chemical communicationThe notion of chemical communication between plants and other organisms has gone from being viewed as a fringe idea to an accepted ecological phenomenon only recently. An Organized Oral Session at the August 2010 Ecological Society of America meeting in Pittsburgh examined the role of plant signall...2011
468 Olivera, Baldomero M.Poly (ADP-ribosylation) in Vitro: reaction parameters and enzyme mechanismThe reaction catalyzed by poly(ADP-ribose) synthetase from bovine thymus was investigated using the purified enzyme, calf thymus DNA, and histone H1. The rate and extent of poly(ADP-ribosylation), as well as the spectrum of acceptor proteins, changed as reaction conditions were varied. The ionic co...ADP-ribosylation; Poly(ADP-ribose) synthetase; Histone H11982
469 Olivera, Baldomero M.Poly(ADP-ribosylation) of a DNA topoisomeraseA DNA topoisomerase activity, copurifying with poly(ADP-ribose) synthetase from calf thymus, >95% inhibited if extensive poly(ADP-ribosylation) is allowed to occur. The inhibited DNA topoisomerase, which has drastically different elution properties on hydroxylapatite, can be reactivated by mild alka...Nicotinic acid; ADP-ribosylation; Turnover cycle; Synthetases1983
470 Olivera, Baldomero M.Poly(ADP-ribosylation) of DNA topoisomerase I from calf thymusWe demonstrate that the activity of the major DNA topoisomerase I from calf thymus is severely inhibited after modification by purified poly(ADP-ribose) synthetase. Polymeric chains of poly(ADP-ribose) are covalently attached to DNA topoisomerase I. These observations with highly purified enzymes ...ADP-ribosylation; Poly(ADP-ribose) synthetase1984
471 Olivera, Baldomero M.Poly(ADP-ribosylation) of DNA topoisomerase I: a nuclear response to DNA-strand interruptionsDNA in eukaryotic chromosomes is organized differently from prokaryotic DNA. It seems highly probable that mechanistic adjustments have been made in macromolecular DNA metabolism as a consequence of the chromatin structure of the eukaryotic chromosome (Igo-Kemenes et al. 1982)). This paper deals ...Poly(ADP-ribosylation); Poly(ADP-ribose) synthetase; DNA strand interruption1984
472 Capecchi, Mario R.Polypeptide chain termination in vitro: isolation of a release factor.The growing polypeptide chain remains bound to the ribosome-messenger RNA complex through the sRNA carrying the last amino acid incorporated into the polypeptide chain.' On completion of the polypeptide chain a mechanism must exist for releasing it from the protein-synthesizing machinery. To date, m...Carbon Isotopes; Phenylalanine; Proteins1967-09-01
473 Capecchi, Mario R.Polypetide chain termination. Purification of the release factors, R1 and R2, from Escherichia coli.We have extensively purified the release factors RI and Rz from Escherichia coli. These proteins can each mediate polypeptide chain termination. The physiological substrate for this reaction is a completed polypeptide chain in a peptidyl- transfer RNA-messenger RNA-ribosome complex. The reaction con...Acrylates; Detergents1971-02-25
474 Ehleringer, James R.Population- and family-level variation of brittlebush (Encelia farinosa, Asteraceae) pubescence: its relation to drought and implications for selection in variable environmentsBecause leaf pubescence of the desert shrub Encelia farinosa increases in response to drought and influences photosynthesis and transpiration, we hypothesized that differences in water availability across the range of this species may result in genetic differentiation for pubescence and associated p...Encelia farinose; Absorptance; Adaptation; Asteraceae; Desert; Drought acclimation; Leaf pubescence; maternal family variance; spatiotemporal heterogeneity2003
475 Olivera, Baldomero M.; Yoshikami, Doju; Bulaj, GrzegorzPost-translational amino acid isomerization: a functionally important D-amino acid in an excitatory peptideThe post-translational modification of an L- to a D-amino acid has been documented in relatively few gene products, mostly in small peptides under 10 amino acids in length. In this report, we demonstrate that a 46-amino acid polypeptide toxin has one D-phenylalanine at position 44, and that the ...D-amino acid; Excitatory peptides; Conotoxins2005
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