Creator | Title | Description | Subject | Date | ||
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1 |
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Capecchi, Mario R. | Establishment of mammalian cell lines containing multiple nonsense mutations and functional suppressor tRNA genes. | We describe the generation of mammalian cell lines carrying amber suppressor genes. Nonsense mutants in the herpes simplex virus thymidine kinase (HSV tk) gene, the Escherichia coli xanthine-guanine phosphoribosyl transferase (Eco-gpt) gene and the aminoglycoside 3' phosphotransferase gene of the Tn... | Cercopithecus aethiops; Escherichia coli; Xenopus | 1982-11-30 |
2 |
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Capecchi, Mario R. | Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells. | We have used site-specific mutagenesis to change the anticodon of a Xenopus laevis tyrosine tRNA gene so that it would recognize ochre codons. This tRNA gene is expressed when amplified in monkey cells as part of a SV40 recombinant and efficiently suppresses termination at both the ochre codon separ... | Xenopus; Nucleic Acid Conformation; Kidney; DNA Restriction Enzymes | 1984-11 |