Human interstitial retinoid-binding protein. Gene structure and primary structure

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Ophthalmology; Neurobiology & Anatomy; Biology
Creator Baehr, Wolfgang
Other Author Liou, Gregory I.; Ma, Din-Pow P.; Yang, Yau-Wen.; Geng, Li.; Zhu, Cihui
Title Human interstitial retinoid-binding protein. Gene structure and primary structure
Date 1989-05-15
Description Interstitial retinoid-binding protein (IRBP) is synthesized and secreted by rod photoreceptor cells into the interphotoreceptor matrix and is known to bind retinoids and fatty acids. We have used cDNA clones encoding human IRBP to isolate a 15-kilobase genomic fragment that encompasses the complete human IRBP gene. The IRBP gene spans more than 11 kilobases and is interrupted by three introns, all of which are positioned near the 3'-end of the coding sequence. The 3741-base pair coding region of IRBP appears to have been generated by quadruplication of an approximately 900 base pair long ancestral gene. The deduced amino acid sequence predicts a mature protein of 1,230 residues (calculated molecular weight 133,000). The protein sequence can be aligned into four homologous segments, each consisting of about 300 residues. Sequence similarity between segments is as high as 60% when conservative substitutions are taken into account. Two putative N-linked glycosylation sites are located in highly conserved domains in the center of the first and second segment of IRBP. A domain consisting of 41 residues at the COOH-terminal end of the third segment has 15 matching residues (38%) with an intradiscal loop of rhodopsin, a retinal-binding protein in rod photoreceptors.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 264
Issue 14
First Page 8200
Last Page 8206
Subject Molecular Sequence Data; Eye Proteins; Retinol-Binding Proteins; Messenger RNA
Subject MESH Protein Biosynthesis; Molecular Sequence Data; Eye Proteins; Transcription, Genetic
Language eng
Bibliographic Citation Liou GI, Ma DP, Yang YW, Geng L, Zhu C, Baehr W. (1989). Human interstitial retinoid-binding protein. Gene structure and primary structure. J Biol Chem, 264(14), 8200-6
Rights Management (c)American Society for Biochemistry and Molecular Biology (ASBMB)
Format Medium application/pdf
Identifier ir-main,1706
ARK ark:/87278/s6pr8dnt
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 707412
Reference URL https://collections.lib.utah.edu/ark:/87278/s6pr8dnt
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