Malignant glioma-derived soluble factors regulate proliferation of normal adult human astrocytes

Update item information
Publication Type Journal Article
School or College School of Medicine
Department Neurosurgery
Creator Couldwell, William T.
Other Author Fraser, Garnet; De Vellis, Genvieve; Antel, Jack P.; Villemure, Jean-Guy; Yong, Voon Wee
Title Malignant glioma-derived soluble factors regulate proliferation of normal adult human astrocytes
Date 1992
Description Malignant gliomas are characteristically surrounded by marked gliosis. To assess whether glioma-derived products contribute to the proliferation of astrocytes, a feature of the gliosis response, we evaluated the influence of culture supernatants from malignant human glioma lines and tumor cyst fluids collected from two patients with glioblastoma multiforme on the proliferation of non-transformed adult human astrocytes. Both the culture supernatants and cyst fluids significantly increased DNA synthesis in astrocytes as assessed by a double immunofluorescence glial fibrillary acidic protein-bromodeoxyuridine technique. The net proliferative effect mediated by glioma cell line supernatants was tumor growth phase-dependent, being preferentially expressed during the logarithmic phase of glioma cell growth. Specific growth factor molecules and cytokines known to be secreted by gliomas (epidermal growth factor, fibroblast growth factor, platelet-derived growth factor, transforming growth factor-β, interleukin-6, and tumor necrosis factor-α) could not reproduce the autogenic effects of the glioma-derived soluble factors. Cytokines which can induce DNA synthesis by adult human astrocytes in vitro, gamma-interferon and interleukin-1, were not detected in the culture supernatant of giioma lines used in this study. In conjunction with the documented effects of glioma products on endothelial and lymphoid cells, the current study suggests that soluble glioma products can contribute to the production of surrounding gliosis observed in vivo.
Type Text
Publisher Wolters Kluwer (LWW)
Volume 51
Issue 5
First Page 506
Last Page 513
Subject Astrocyte; Glioma; Gliosis; Proliferation; Soluble factors
Subject LCSH Gliomas; Astrocytes
Language eng
Bibliographic Citation Couldwell, W. T., Fraser, G., De Vellis, G., Antel, J. P., Villemure, J.-G., & Yong, V. W. (1992). Malignant glioma-derived soluble factors regulate proliferation of normal adult human astrocytes. Journal of Neuropathology & Experimental Neurology, 51(5), 506-13.
Rights Management (c) Wolters Kluwer (LWW)
Format Medium application/pdf
Format Extent 1,443,264 bytes
Identifier ir-main,12498
ARK ark:/87278/s62522w3
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 707386
Reference URL https://collections.lib.utah.edu/ark:/87278/s62522w3
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