Arf 1 dissociates from the clathrin adaptor GGA prior to being inactivated by Arf GTPase-activating proteins

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Publication Type Journal Article
School or College College of Health
Department Exercise & Sport Science
Creator Jacques, Kerry M.
Other Author Nie, Zhongzhen; Stauffer, Stacey; Hirsch, Dianne S.; Chen, Ling-Xin; Stanley, Katherine T.; Randazzo, Paul A.
Title Arf 1 dissociates from the clathrin adaptor GGA prior to being inactivated by Arf GTPase-activating proteins
Date 2002
Description The effectors of monomeric GTP-binding proteins can influence interactions with GTPase-activating proteins (GAPs) in two ways. In one case, effector and GAP binding to the GTP-binding protein is mutually exclusive. In another case, the GTP-binding protein bound to an effector is the substrate for the GTPase-activating protein. Here predictions for these two mechanisms were tested for the Arf1 effector GGA and ASAP family Arf GAPs. GGA inhibited Arf GAP activity of ASAP1, AGAP1, ARAP1, and Arf GAP1 and inhibited binding of Arf1?GTPyS to AGAP1 with Ki values correlating with the Kd for the GGA?Arf1 complex. ASAP1 blocked Arf1?GTPyS binding to GGA with a Ki similar to the Kd for the ASAP?Arf1?GTPyS complex. No interaction of GGA with ASAP1 was detected. Consistent with GGA sequestering Arf from GAPs, overexpression of GGA slowed the rate of Arf dissociation from the Golgi apparatus following treatment with brefeldin A. Mutational analysis revealed the amino-terminal ?-helix and switch I of Arf1 contributed to interaction with both GGA and GAPs. These data exclude the mechanism previously documented for Arf GAP1/coatomer in which Arf1 is inactivated in a tripartite complex. Instead, termination of Arf1 signals mediated through GGA require that Arf1?GTP dissociates from GGA prior to interaction with GAP and consequent hydrolysis of GTP.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
Volume 277
Issue 49
First Page 47235
Last Page 47241
Language eng
Bibliographic Citation Jacques, K. M., Nie, Z., Stauffer, S., Hirsch, D. S., Chen, L. X., Stanley, K. T., & Randazzo, P. A. (2002). Arf 1 dissociates from the clathrin adaptor GGA prior to being inactivated by Arf GTPase-activating proteins. Journal of Biological Chemistry, 277(49), 47235-41.
Rights Management ¬©American Society for Biochemistry and Molecular Biology (ASBMB) http://www.asbmb.org/
Format Medium application/pdf
Format Extent 191,320 bytes
Identifier ir-main,16692
ARK ark:/87278/s6z03ss5
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 707263
Reference URL https://collections.lib.utah.edu/ark:/87278/s6z03ss5
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