Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay results

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Publication Type Journal Article
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Jaskowski, Troy D.
Other Author Willman, Joseph H.; Hill, Harry R.; Martins, Thomas B.; Ashwood, Edward R.; Litwin, Christine M.
Title Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay results
Date 2001
Description We hypothesized that heterophil antibodies reactive with animal proteins used in blot preparation caused nonspecific staining (NSS) on HIV Western blot (WB) studies, causing indeterminate results. We analyzed samples showing NSS on HIV WB using a multiplexed immunoassay to simultaneously measure IgG antibodies to animal IgG (bovine, goat, sheep, mouse) and bovine serum albumin. Heterophil antibodies reactive with IgG from several animal species were detected in 23 (49%) of 47 samples showing NSS on HIV WB; 15 positive samples demonstrated antibodies to all 5 antigens. Similar IgG heterophil antibodies were detected in only 2 (8%) of 24 control samples. Of the HIV WB samples with a positive HIV-1 enzymelinked immunosorbent assay (ELISA) result at the time of WB testing (11/47), heterophil antibodies were found in 8 (73%) of 11. Preabsorption with bovine, goat, and sheep IgG removed heterophil antibodies detected by the multiplexed assay and, in some cases, eliminated reactivity in ELISA and WB testing. Heterophil antibodies are associated with indeterminate HIV immunoassay results and are an important cause of false-positive HIV ELISA results. Multiplexed immunoassays provide a powerful tool for screening patients for heterophil antibodies and resolving possible false-positive results.
Type Text
Publisher American Society for Clinical Pathology
Volume 115
Issue 5
First Page 764
Last Page 769
Subject Heterophil antibodies; Heterophil interference; Multiplex analysis; False-positive HIV enzyme-linked immunosorbent assay; ELISA
Subject LCSH Immunoassay; Immunodeficiency; HIV infections; HIV infections -- Diagnosis
Language eng
Bibliographic Citation Willman, J. H., Hill, H. R., Martins, T. B., Jaskowski, T. D., Ashwood, E. R., & Litwin, C. M. (2001). Multiplex analysis of heterophil antibodies in patients with indeterminate HIV immunoassay results. American Journal of Clinical Pathology, 115(5), 764-9.
Rights Management ©1996-2010 American Society for Clinical Pathology and ©1996-2010 ASCP Press
Format Medium application/pdf
Format Extent 104,513 bytes
Identifier ir-main,11450
ARK ark:/87278/s6th959n
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 707202
Reference URL https://collections.lib.utah.edu/ark:/87278/s6th959n
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