Adaptation of the protein kinase filter paper assay to a 96-well microtiter format.

Publication Type Journal Article
School or College College of Pharmacy; College of Engineering; School of Medicine
Department Biochemistry; Biomedical Informatics; Bioengineering; Pharmacology & Toxicology
Creator Blumenthal, Donald K.; Underwood, Clayton J.
Title Adaptation of the protein kinase filter paper assay to a 96-well microtiter format.
Date 1999-02-01
Description The most widely used method for assaying protein kinase activities involves incorporation of radioactive phosphate into a protein or peptide substrate with subsequent binding or precipitaion of the radiolabeled substrate onto filter paper squares. We have adapted this assay for use with readily available 96-well microtiter plate technologies. The sensitivity and reproducibility of the modified 96-well protein kinase assay are comparable to standard filter paper assay methods, but assay throughput is much greater and reagent costs are significantly reduced. This technique has been used to assay several different protein kinase activities using both P81 phosphocellulose and 3MM filter papers. The general methods described here could be readily adapted for use with other kinds of filter materials or used with automated assay systems.
Type Text
Publisher Academic Press
Volume 267
Issue 1
First Page 235
Last Page 238
Subject Protein Kinase Activities; Filter Papers; Radioactive Phosphate
Subject MESH Amino Acid Sequence; Indicators and Reagents; Microchemistry; Phosphorus Radioisotopes; Protein Kinases; Scintillation Counting; Substrate Specificity
Language eng
Bibliographic Citation Underwood CJ, Blumenthal DK. Adaptation of the protein kinase filter paper assay to a 96-well microtiter format. Anal Biochem. 1999 Feb 1;267(1):235-8. Retrieved September 15, 2006 from http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W9V-45N4K22-12&_coverDate=02%2F01%2F1999&_alid=493029216&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6692&_sort=d&view=c&_acct=C000049956&_version=1&_urlVersion=0&_userid=990933&md5=6734268a68aa3b43d3b4deb8d1d9f93c
Format Medium application/pdf (portable document)
Identifier ir-main,401
ARK ark:/87278/s68349p7
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 707129
Reference URL https://collections.lib.utah.edu/details?id=707129
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