Performance characteristics of eight estradiol immunoassays

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Publication Type Journal Article
Department Pathology
Program ARUP Institute for Clinical and Experimental Pathology
Creator Owen, William E.; Roberts, William L.
Other Author Yang, David T.; Ramsay, Carol S.; Xie, Hui
Title Performance characteristics of eight estradiol immunoassays
Date 2004
Description Measurement of estradiol is useful in assisted reproduction, evaluation of infertility, menopause, and male feminization. The analytic performance of 8 estradiol immunoassays was evaluated. The imprecision and accuracy of the Access, ADVIA Centaur, ARCHITECT i2000, AutoDELFIA, Elecsys 2010, IMMULITE 2000, and Vitros ECi estradiol assays (see text for proprietary information) were evaluated by using an isotope dilution-gas chromatography-mass spectrometry (ID-GC-MS) reference method. The coefficient of variation (CV) ranged from 6.9% on the Elecsys 2010 to 42.6% on the ADVIA Centaur at an estradiol concentration of 18 pg/mL (66 pmol/L), with the ARCHITECT i2000 assay in development and the Vitros ECi having a CV below 10% at this estradiol concentration. Agreement between the automated assays and ID-GC-MS was variable, with slopes ranging from 0.87 to 1.20. The Access, ARCHITECT i2000 in development, and the IMMULITE 2000 were the most accurate, with slopes of 0.99, 0.98, and 1.03, respectively. These findings indicate that the ARCHITECT i2000 estradiol assay in development had the best precision and accuracy of the assays evaluated for measurement of serum estradiol concentrations.
Type Text
Publisher American Society for Clinical Pathology
Volume 122
Issue 3
First Page 332
Last Page 337
Subject Estradiol immunoassays
Subject LCSH Estradiol; Immunoassay; Immunoassay -- Evaluation
Language eng
Bibliographic Citation Yang, D. T., Owen, W. E., Ramsay, C. S., Xie, H., & Roberts, W. L. (2004). Performance characteristics of eight estradiol immunoassays. American Journal of Clinical Pathology, 122(3), 332-7.
Rights Management ©2003-2008 American Society for Clinical Pathology and ©2003-2008 ASCP Press
Format Medium application/pdf
Format Extent 358,375 bytes
Identifier ir-main,11551
ARK ark:/87278/s6c544g3
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 707074
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