Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Internal Medicine; Neurobiology & Anatomy; Oncological Sciences; Human Genetics; Biology
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.; Mansour, Suzanne L.; Thomas, Kirk R.
Title Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes.
Date 1988-11-24
Description Gene targeting--homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences--in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive nd negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation of hprt- and int-2- mutants, but it should be applicable to any gene.
Type Text
Publisher Nature Publishing Group
Volume 336
Issue 6197
First Page 348
Last Page 352
Subject Animals; Clone Cells; Restriction Mapping; Stem Cells
Subject MESH Mice, Transgenic; Mutation; Proto-Oncogenes; Transfection
Language eng
Bibliographic Citation Nature. 1988 Nov 24;336(6197):348-52. Mansour SL, Thomas KR, Capecchi MR. Disruption of the proto-oncogene int-2 in mouse embryo-derived stem cells: a general strategy for targeting mutations to non-selectable genes. Retrieved on November 1 2006 from http://www.nature.com/nature/journal/v336/n6197/abs/336348a0.html.
Rights Management copyright © 1988 Nature Publishing Group. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,519
ARK ark:/87278/s60p1hnh
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706924
Reference URL https://collections.lib.utah.edu/ark:/87278/s60p1hnh
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