Cross-species comparison of metabolite profiles in chemosensory epithelia: an indication of metabolite roles in chemosensory cells

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Publication Type Journal Article
School or College School of Medicine
Department Physiology
Creator Lucero, Mary T.; Mobley, Arie S.
Other Author Michel, William
Title Cross-species comparison of metabolite profiles in chemosensory epithelia: an indication of metabolite roles in chemosensory cells
Date 2008-04
Description Comparative studies of chemosensory systems in vertebrates and invertebrates have greatly enhanced our understanding of anatomical and physiological constraints of chemical detection. Immunohistochemical comparisons of chemosensory systems are difficult to make across species due to limited cross-reactivity of mammalian-based antibodies. Immunostaining chemosensory tissues with glutaraldehyde-based antibodies generated against small metabolites in combination with hierarchical cluster analyses provide a novel approach for identifying and classifying cell types regardless of species. We used this "metabolite profiling" technique to determine whether metabolite profiles can be used to identify cell classes within and across different species including mouse, zebrafish, lobster and squid. Within a species, metabolite profiles for distinct cell classes were generally consistent. We found several metabolite-based cell classifications that mirrored function or receptor protein-based classifications. Although profiles of all six metabolites differed across species, we found that specific metabolites were associated with certain cell types. For example, elevated levels of glutathione were characteristic of nonsensory cells from vertebrates, suggesting an antioxidative role in non-neuronal cells in sensory tissues. Collectively, we found significantly different metabolite profiles for distinct cell populations in chemosensory tissue within all of the species studied. Based on their roles in other systems or cells, we discuss the roles of L-arginine, L-aspartate, L-glutamate, glycine, glutathione, and taurine within chemosensory epithelia.
Type Text
Publisher John Wiley & Sons
Subject Olfactory; Vomeronasal; Mouse; Zebrafish; Squid
Subject MESH Biological Markers; Chemoreceptors; Immunohistochemistry; Species Specificity; Epithelial Cells
Language eng
Bibliographic Citation Mobley A. S., Lucero M.T. & Michel W. C. (2008) A Cross-Species Comparison of Metabolite Profiles in Chemosensory Epithelia: an indication of metabolite roles in chemosensory cells. The Anatomical Record. (In press)
Rights Management (c) John Wiley & Sons
Format Medium application/pdf
Identifier ir-main,14883
ARK ark:/87278/s6mw31rg
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 706836
Reference URL