Inducible nitric oxide synthase (NOS2) promoter CCTTT repeat polymorphism: relationship to in vivo nitric oxide production/NOS activity in an asymptomatic malaria-endemic population

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Publication Type Journal Article
School or College School of Medicine
Department Internal Medicine
Creator Hobbs, Maurine R.; Granger, Donald Lee
Other Author Boutlis, Craig S; Marsh, Robyn L; Misukonis, Mary A; Tkachuk, Ariana N; Lagog, Moses; Booth, Jennifer; Mgone, Charles S; Levesque, Marc C; Weinberg, J Brice; Anstey, Nicholas M
Title Inducible nitric oxide synthase (NOS2) promoter CCTTT repeat polymorphism: relationship to in vivo nitric oxide production/NOS activity in an asymptomatic malaria-endemic population
Date 2003
Description Polymorphisms in the inducible nitric oxide synthase gene (NOS2) promoter have been associated with clinical outcome from malaria. These include a CCTTT repeat (CCTTTn) 2.5 kilobases upstream from the NOS2 transcription start site, and two single nucleotide substitutions: G-->C at position -954 (G-954C), and C-->T at position -1173 (C-1173T). Although hypothesized to influence NO production in vivo, the functional relevance of (CCTTT)n and G-954C is uncertain because disease association studies have yielded inconsistent results. This study found no association between CCTTT repeat number and levels of plasma NO metabolites or peripheral blood mononuclear cell NOS activity in a cohort of asymptomatic malaria-exposed coastal Papua New Guineans 1-60 years old. This suggests that (CCTTT)n does not independently influence NOS2 transcription in vivo. Neither the G-954C nor the C-1173T polymorphisms were identified in this population, indicating the variability and complexity of selection for NOS2 promoter polymorphisms in different malaria-endemic populations.
Type Text
Publisher American Society for Tropical Medicine and Hygiene
Volume 69
Issue 6
First Page 569
Last Page 573
Subject Papua New Guinea; Oceanic Ancestry Group; Repetitive Sequences, Nucleic Acid
Subject MESH Genetic Predisposition to Disease; Nitric Oxide; Polymorphism, Genetic
Language eng
Bibliographic Citation Boutlis CS, Hobbs MR, Marsh RL, Misukonis MA, Tkachuk AN, Lagog M, Booth J, Granger DL, Bockarie MJ, Mgone CS, Levesque MC, Weinberg JB, Anstey NM. (2003) Inducible nitric oxide synthase (NOS2) promoter CCTTT repeat polymorphism: relationship to in vivo nitric oxide production/NOS activity in an asymptomatic malaria-endemic population. Am J Trop Med Hyg, 69(6):569-73
Rights Management (c) American Society for Tropical Medicine and Hygiene
Format Medium application/pdf
Identifier ir-main,1708
ARK ark:/87278/s654366r
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706771
Reference URL https://collections.lib.utah.edu/ark:/87278/s654366r
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