Nucleic acid vaccine encoding gD2 protects mice from herpes simplex virus type 2 disease.

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Publication Type Journal Article
School or College School of Medicine
Department Dermatology; Internal Medicine; Ophthalmology; Pathology
Creator Kriesel, John D.; Spruance, Spotswood L.; Daynes, Raymond A.
Other Author Araneo, B. A.
Title Nucleic acid vaccine encoding gD2 protects mice from herpes simplex virus type 2 disease.
Date 1996
Description Nucleic acid vaccinations with plasmids pWW65, containing the sequence for herpes simplex type 2 (HSV-2) gD2, and pRSVnt, lacking the gD sequence, were studied. Groups of mice were immunized with pWW65 alone, pWW65 plus 1,25-dihydroxyvitamin-D3 (D3), or pRSVnt. Clinical disease (vaginitis), serum and vaginal washing antibody levels, and vaginal washing virus titers were measured intravaginal HSV-2 challenge. No animals (0/10) in the pWW65 + D3 group, 6/10 animals in the pWW65 group, and 10/10 animals in the pRSVnt group developed severe disease by postchallenge day 13 (P<.001, P=.04 vs. pRSVnt). Virus titers in vaginal washings were significantly reduced in the pWW65 and pWW65+D3 groups versus the pRSVnt group (P<.001). Increasing levels of serum anti-gD2 antibodies were measured 2 and 6 days after challenge among animals in the pWW65 and pWW65+D3 groups but not among animals in the pRSVnt group. Vaccinations with a plasmid containing the gD2 gene are immunogenic and provide some protection from HSV-2-induced disease.
Type Text
Publisher University of Chicago Press
Volume 173
Issue 3
First Page 536
Last Page 541
Subject Mice, Inbred BALB C; Plasmids; Viral Vaccines
Subject MESH DNA, Viral; Herpes Genitalis; Simplexvirus
Language eng
Bibliographic Citation Kriesel JD, Spruance SL, Daynes RA, Araneo BA. (1996). Nucleic acid vaccine encoding gD2 protects mice from herpes simplex virus type 2 disease. J Infect Dis, 173(3), 536-41.
Rights Management (c) University of Chicago Press http://www.journals.uchicago.edu/loi/jid
Format Medium application/pdf
Identifier ir-main,1768
ARK ark:/87278/s6f19h83
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706676
Reference URL https://collections.lib.utah.edu/ark:/87278/s6f19h83
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