Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Hughes, Stephen H.; Wahl, Geoffrey M.
Title Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro.
Date 1975-11
Description tRNA isolated from two different yeast super-suppressor strains translates a known nonsense mutation in vitro, whereas tRNA from a closely related nonsuppressing strain does not. Suppression was assayed by translation of RNA isolated from an amber coat mutant of bacteriophage Qbeta (GB11) in a protein-synthesizing system derived from mouse tissue culture cells (L cells). Suppressed forms of Qbeta coat protein synthesized in vitro were quantitatively detected by a specific immunoprecipitation assay. The L-cell protein-synthesizing system also responds to E. coli suppressor tRNA. This indicates that the biochemical mechanism for nonsense suppression is very similar in yeast and E. coli. These findings also provide additional evidence that the amber codon (UAG) functions as one of the mammalian chain-terminating codons. Since the suppression assay utilizes protein-synthesizing components isolated from mammalian cells, it should prove useful in the search for mammalian nonsense suppressors
Type Text
Publisher MIT
Volume 6
Issue 3
First Page 269
Last Page 277
Subject Codon; Escherichia coli; Protein Biosynthesis
Subject MESH RNA, Transfer; Saccharomyces cerevisiae; Suppression, Genetic
Language eng
Bibliographic Citation Cell. 1975 Nov;6(3):269-77: Capecchi MR, Hughes SH, Wahl GM. Yeast super-suppressors are altered tRNAs capable of translating a nonsense codon in vitro. Retrieved on September 25.2006 from http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-4C8G37J-2R&_coverDate=11%2F30%2F1975&_alid=455503479&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=7051&_sort=d&view=c&_acct=C000049956&_version=1&_urlVersion=0&_userid=990933&md5=8df7f86fe50bcb9d1e0a5d2913cde0ea
Rights Management Copyright © 1975, MIT. All Rights Reserved.
Format Medium application/pdf
ARK ark:/87278/s67h2353
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706662
Reference URL https://collections.lib.utah.edu/ark:/87278/s67h2353
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