Programmed frameshifting in the synthesis of mammalian antizyme is +1 in mammals, predominantly +1 in fission yeast but "2 in budding yeast

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Publication Type Journal Article
School or College School of Medicine
Department Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Gesteland, Raymond F.; Ivanov, Ivaylo P.; Atkins, John F.
Other Author Matsufuji, Senya
Title Programmed frameshifting in the synthesis of mammalian antizyme is +1 in mammals, predominantly +1 in fission yeast but "2 in budding yeast
Date 1998
Description The coding sequence for mammalian ornithine decarboxylase antizyme is in two different partially overlapping reading frames with no independent ribosome entry to the second ORF. Immediately before the stop codon of the first ORF, a proportion of ribosomes undergo a quadruplet translocation event to shift to the +1 reading frame of the second and main ORF. The proportion that frameshifts is dependent on the polyamine level and, because the product antizyme is a negative regulator of intracellular polyamine levels, the frameshifting acts to complete an autoregulatory circuit by sensing polyamine levels. An mRNA element just 59 of the shift site and a 39 pseudoknot are important for efficient frameshifting. Previous work has shown that a cassette with the mammalian shift site and associated signals directs efficient shifting in the budding yeast Saccharomyces cerevisiae at the same codon to the correct frame, but that the shift is "2 instead of +1. The product contains an extra amino acid corresponding to the shift site. The present work shows efficient frameshifting also occurs in the fission yeast, Schizosaccharomyces pombe. This frameshifting is 80% +1 and 20% "2. The response of S. pombe translation apparatus to the mammalian antizyme recoding signals is more similar to that of the mammalian system than to that of S. cerevisiae. S. pombe provides a good model system for genetic studies on the mechanism of at least this type of programmed mammalian frameshifting.
Type Text
Publisher Cold Spring Harbor Lab Press
First Page 1230
Last Page 1238
Subject Antizyme; Frameshifting; Ornithine decarboxylase antizyme; Recoding; S. pombe
Subject MESH Frameshifting, Ribosomal; Ornithine Decarboxylase
Language eng
Bibliographic Citation Ivanov, I.P., Gesteland, R.F., Matsufuji, S., and Atkins, J.F. 1998. Programmed frameshifting in the synthesis of mammalian antizyme is +1 in mammals, predominantly +1 in fission yeast but "2 in budding yeast. RNA 4:1230-1238
Rights Management (c) Cold Spring Harbor Laboratory Press-CSHL Press
Format Medium application/pdf
Format Extent 702,843 Bytes
Identifier ir-main,3742
ARK ark:/87278/s6q5376g
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 706551
Reference URL https://collections.lib.utah.edu/ark:/87278/s6q5376g
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