High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Thomas, Kirk R.; Capecchi, Mario R.
Other Author Deng, Chuxia
Title High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors.
Date 1992-07
Description Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides by several kilobases of DNA homologous to the target locus, replacement of chromosomal sequences with the exogenous DNA occurred with precision. If, on the other hand, the target-homologous DNA on one arm of the vector was reduced to below 1 kb in length, the fidelity of recombination was diminished.
Type Text
Publisher American Society for Microbiology
Volume 12
Issue 7
First Page 2919
Last Page 2923
Subject Drug Resistance; Gene Rearrangement; Gentamicins
Subject MESH Genetic Vectors; Mutagenesis, Site-Directed; Stem Cells
Language eng
Bibliographic Citation Mol Cell Biol. 1992 Jul;12(7):2919-23: Thomas KR, Deng C, Capecchi MR. High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. Retrieved on September 15.2006 from http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1620105.
Rights Management Copyright © 1992, American Society for Microbiology. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,425
ARK ark:/87278/s6vh66dk
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 706429
Reference URL https://collections.lib.utah.edu/ark:/87278/s6vh66dk
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