Publication Type |
Journal Article |
School or College |
College of Science |
Department |
Chemistry |
Creator |
Poulter, Charles Dale |
Other Author |
Chen, Anjun |
Title |
Purification and characterization of farnesyl diphosphate/geranylgeranyl diphosphate synthase |
Date |
1993-05-25 |
Description |
Farnesyl diphosphate (FPP)/geranylgeranyl diphosphate (GGPP) synthase, a bifunctional enzyme that synthesizes C15 and C2o isoprenoid diphosphates from isopentenyl diphosphate and dimethylallyl diphosphate, was purified to homogeneity from the archae-bacterium Methanobacterium thermoautotrophicum. The only activities detected from synthesis of FPP and GGPP copurified through (NH4)2S04 precipitation and four chromatographic steps. The pure enzyme was a 79-kDa homodimer that catalyzed the sequential addition of isopentenyl diphosphate to dimethylallyl diphosphate, geranyl diphosphate, and FPP by a non-processive mechanism which allowed substantial amounts of FPP to accumulate during turnover, creating a pool for further elongation to GGPP or for synthesis of squalene. The bifunctional enzyme required Mg2+ or Mn2+ and was optimally active at 65 °C. Catalysis of chain elongation in M. thermoautotrophicum differs from related reactions in eubacteria and eukaryotes, where distinct FPP synthases and GGPP synthases are found. |
Type |
Text |
Publisher |
American Society for Biochemistry and Molecular Biology (ASBMB) |
First Page |
11002 |
Last Page |
11007 |
Subject |
Bifunctional enzyme; Methanobacterium thermautotrophicum; Synthases |
Subject LCSH |
Methanothermobacter thermautotrophicus; Archaebacteria; Enzymes |
Language |
eng |
Bibliographic Citation |
Poulter, C. D. (1993). Purification and characterization of farnesyl diphosphate/geranylgeranyl diphosphate synthase. Journal of Biological Chemistry 268, 11002-7. |
Rights Management |
(c)American Society for Biochemistry and Molecular Biology (ASBMB) |
Format Medium |
application/pdf |
Format Extent |
980,046 Bytes |
Identifier |
ir-main,2690 |
ARK |
ark:/87278/s654363d |
Setname |
ir_uspace |
ID |
706004 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s654363d |