Purification and characterization of farnesyl diphosphate/geranylgeranyl diphosphate synthase

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Publication Type Journal Article
School or College College of Science
Department Chemistry
Creator Poulter, Charles Dale
Other Author Chen, Anjun
Title Purification and characterization of farnesyl diphosphate/geranylgeranyl diphosphate synthase
Date 1993-05-25
Description Farnesyl diphosphate (FPP)/geranylgeranyl diphosphate (GGPP) synthase, a bifunctional enzyme that synthesizes C15 and C2o isoprenoid diphosphates from isopentenyl diphosphate and dimethylallyl diphosphate, was purified to homogeneity from the archae-bacterium Methanobacterium thermoautotrophicum. The only activities detected from synthesis of FPP and GGPP copurified through (NH4)2S04 precipitation and four chromatographic steps. The pure enzyme was a 79-kDa homodimer that catalyzed the sequential addition of isopentenyl diphosphate to dimethylallyl diphosphate, geranyl diphosphate, and FPP by a non-processive mechanism which allowed substantial amounts of FPP to accumulate during turnover, creating a pool for further elongation to GGPP or for synthesis of squalene. The bifunctional enzyme required Mg2+ or Mn2+ and was optimally active at 65 °C. Catalysis of chain elongation in M. thermoautotrophicum differs from related reactions in eubacteria and eukaryotes, where distinct FPP synthases and GGPP synthases are found.
Type Text
Publisher American Society for Biochemistry and Molecular Biology (ASBMB)
First Page 11002
Last Page 11007
Subject Bifunctional enzyme; Methanobacterium thermautotrophicum; Synthases
Subject LCSH Methanothermobacter thermautotrophicus; Archaebacteria; Enzymes
Language eng
Bibliographic Citation Poulter, C. D. (1993). Purification and characterization of farnesyl diphosphate/geranylgeranyl diphosphate synthase. Journal of Biological Chemistry 268, 11002-7.
Rights Management (c)American Society for Biochemistry and Molecular Biology (ASBMB)
Format Medium application/pdf
Format Extent 980,046 Bytes
Identifier ir-main,2690
ARK ark:/87278/s654363d
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2012-06-13
ID 706004
Reference URL https://collections.lib.utah.edu/ark:/87278/s654363d
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