P-site tRNA is a crucial initiator of ribosomal frameshifting

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Publication Type Journal Article
School or College School of Medicine; College of Engineering; College of Science
Department Bioengineering; Biology; Human Genetics
Creator Gesteland, Raymond F.; Atkins, John F.; Baranov, Pavel V.
Title P-site tRNA is a crucial initiator of ribosomal frameshifting
Date 2004
Description The expression of some genes requires a high proportion of ribosomes to shift at a specific site into one of the two alternative frames. This utilized frameshifting provides a unique tool for studying reading frame control. Peptidyl-tRNA slippage has been invoked to explain many cases of programmed frameshifting. The present work extends this to other cases. When the A-site is unoccupied, the P-site tRNA can be repositioned forward with respect to mRNA (although repositioning in the minus direction is also possible). A kinetic model is presented for the influence of both, the cognate tRNAs competing for overlapping codons in A-site, and the stabilities of P-site tRNA:mRNA complexes in the initial and new frames. When the A-site is occupied, the P-site tRNA can be repositioned backward. Whether frameshifting will happen depends on the ability of the A-site tRNA to subsequently be repositioned to maintain physical proximity of the tRNAs. This model offers an alternative explanation to previously published mechanisms of programmed frameshifting, such as out-of-frame tRNA binding, and a different perspective on simultaneous tandem tRNA slippage.
Type Text
Publisher Cold Spring Harbor Lab Press
Volume 10
Issue 2
First Page 221
Last Page 230
Subject Translation; Recoding; Kinetic Model; Frameshifting; Ribosome
Subject MESH Frameshifting, Ribosomal; RNA, Transfer; Reading Frames; Ribosomes
Language eng
Bibliographic Citation Baranov PV, Gesteland RF, Atkins JF. 2004. P-site tRNA is a crucial initiator of ribosomal frameshifting. RNA 10(2):221-30
Rights Management © Cold Spring Harbor Laboratory Press-CSHL Press
Format Medium application/pdf
Identifier ir-main,12753
ARK ark:/87278/s60s06s7
Setname ir_uspace
ID 705162
Reference URL https://collections.lib.utah.edu/ark:/87278/s60s06s7