Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells.

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Publication Type Journal Article
School or College College of Science; School of Medicine
Department Oncological Sciences; Biology; Human Genetics
Program Institute of Human Genetics; Howard Hughes Medical Institute (HHMI)
Creator Capecchi, Mario R.
Other Author Lakki, Frank A.; Belagaje, Ramamoorthy; Hudziak, Robert M.; Norton, Gerard P.; RajBhandary, Uttam L.; Sharp, Phillip A.
Title Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells.
Date 1984-11
Description We have used site-specific mutagenesis to change the anticodon of a Xenopus laevis tyrosine tRNA gene so that it would recognize ochre codons. This tRNA gene is expressed when amplified in monkey cells as part of a SV40 recombinant and efficiently suppresses termination at both the ochre codon separating the adenovirus 2 hexon gene from a 23-kd downstream gene and the ochre codon at the end of the NS1 gene of influenza virus A/Tex/1/68. Termination at an amber codon of a NS1 gene of another influenza virus strain was not suppressed by the (Su+) ochre gene suggesting that in mammalian cells amber codons are not recognized by ochre suppressor tRNAs. Finally, microinjection into mammalian cells of both (Su+) ochre tRNA genes and selectible genes containing ochre nonsense mutations gives rise to colonies under selective conditions. We conclude that it should be possible to isolate a wide assortment of mammalian cell lines with ochre suppressor activity.
Type Text
Publisher IRL Press Limited, Oxford, England.
Volume 3
Issue 11
First Page 2445
Last Page 2452
Subject Xenopus; Nucleic Acid Conformation; Kidney; DNA Restriction Enzymes
Subject MESH Transcription, Genetic; Suppression, Genetic; RNA, Transfer, Amino Acyl; Mutation; Genes, Synthetic
Language eng
Bibliographic Citation EMBO J. 1984 Nov;3(11):2445-52: Laski FA, Belagaje R, Capecchi MR, Norton GP, Palese P, RajBhandary UL, Sharp PA, Synthesis of an ochre suppressor tRNA gene and expression in mammalian cells. Retrieved on September 15.2006 from
Rights Management Copyright 1984 IRL Press Limited, Oxford, England. All Rights Reserved.
Format Medium application/pdf
Identifier ir-main,422
ARK ark:/87278/s62r492v
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 705146
Reference URL
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