Upstream stimulators for recoding

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Publication Type Journal Article
School or College College of Science
Department Human Genetics
Creator Gesteland, Raymond F.; Atkins, John F.; Wills, Norma M.
Other Author Fayet, G.; Peden J.
Title Upstream stimulators for recoding
Date 1995
Description Recent progress in elucidation of 5' stimulatory elements for translational recoding is reviewed. A 5' Shine-Dalgarno sequence increases both +l and ? I frameshift efficiency in several genes; examples cited include the E. coli prfB gene encoding release factor 2 and the thuiX gene encoding the y and t subunits of DNA polymerase III holoenzyme. The spacing between the Shine-Dalgarno sequence and the shift site is critical in both the +l and ? 1 frameshift cassettes; however, the optimal spacing is quite different in the two cases. A frameshift in a mammalian chromosomal gene, ornithine decarboxylase antizyme, has recently been reported; 5' sequences have been shown to be vital for this frameshift event. Escherichia coli bacteriophage T4 gene 60 encodes a subunit of its type II DNA topoisomerase. The mature gene 60 mRNA contains an internal 50 nucleotide region that appears to be bypassed during translation. A 16 amino acid domain of the nascent peptide is necessary for this bypass to occur.
Type Text
Publisher National Research Council Canada Research Press
First Page 1123
Last Page 1129
Subject Recoding; Frameshifting; Peptide factor; Stimulatory elements
Subject MESH Frameshift Mutation; Genetics
Language eng
Bibliographic Citation Larsen, B., Peden, J., Matsufuji, S., Matsufuji, T., Brady, K., Maldonado, R., Wills, N.M., Fayet, O., Atkins, J.F., and Gesteland, R.F. 1995. Upstream stimulators for recoding. Biochem. Cell Biol. 73:1123-1129
Rights Management © National Research Council Canada Research Press
Format Medium application/pdf
Identifier ir-main,3723
ARK ark:/87278/s6t44bhq
Setname ir_uspace
Date Created 2012-06-13
Date Modified 2021-05-06
ID 704971
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