Publication Type |
Journal Article |
Department |
Pathology |
Program |
ARUP Institute for Clinical and Experimental Pathology |
Creator |
Jaskowski, Troy D. |
Other Author |
Schroder, Carl; Martins, Thomas B.; Mouritsen, C. Lars; Litwin, Christine M.; Hill, Harry R. |
Title |
Screening for antinuclear antibodies by enzyme immunoassay |
Date |
1996 |
Description |
Indirect fluorescent antibody (IFA) is the most widely used method in clinical laboratories to screen for autoantibodies against a wide variety of nuclear antigens. Recently, a number of antinuclear antibody (ANA) enzyme immunoassay (EIA) screens have become commercially available and claim to be an alternative method to screen for ANAs. Given the subjectivity of technical interpretation of IFA and the high number of ANA negative samples, a suitable EIA method for ANA screening would be beneficial to clinical laboratories with large sample volumes. Five ANA EIA screens were compared (Efias, Helix, Sanofi, ThcraTcst and Zeus) to IFA using a human epithelial cell line (HEp-2). Sera from 601 patients submitted to our reference laboratory for autoimmune testing, and from 202 normal healthy blood donors, were included in this study. Samples with discordant results between IFA and EIA were further analyzed using single antigen EIAs for SSA, SSB, Sm, RNP, Scl-70, histones, dsDNA, and ssDNA. |
Type |
Text |
Publisher |
American Society for Clinical Pathology |
Volume |
105 |
Issue |
4 |
First Page |
468 |
Last Page |
473 |
Subject |
Antinuclear antibodies; Enzyme immunoassay |
Subject LCSH |
Antinuclear factors; Autoantibodies; Immunoenzyme technique; Immunoassay |
Language |
eng |
Bibliographic Citation |
Jaskowski, T. D., Schroder, C., Martins, T. B., Mouritsen, C. L., Litwin, C. M., & Hill, H. R. (1996). Screening for antinuclear antibodies by enzyme immunoassay. American Journal of Clinical Pathology, 105(4), 468-73. |
Rights Management |
©1996-2010 American Society for Clinical Pathology and ©1996-2010 ASCP Press |
Format Medium |
application/pdf |
Format Extent |
634,892 bytes |
Identifier |
ir-main,11461 |
ARK |
ark:/87278/s6sq9hst |
Setname |
ir_uspace |
ID |
704855 |
Reference URL |
https://collections.lib.utah.edu/ark:/87278/s6sq9hst |